Endophytes, associated compositions, and methods of use thereof
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-017/00
A01N-063/04
C05F-011/08
A01N-063/00
A01N-063/02
출원번호
US-0752829
(2015-06-26)
등록번호
US-10212911
(2019-02-26)
발명자
/ 주소
von Maltzahn, Geoffrey
Sadowski, Craig
Lyford, Jeffrey
Ambrose, Karen V.
Zhang, Xuecheng
Flavell, Richard Bailey
Toledo, Gerardo V.
Leff, Jonathan W.
Samayoa, Phillip
Marquez, Luis Miguel
Johnston, David Morris
Djonovic, Slavica
Millet, Yves Alain
출원인 / 주소
Indigo Agriculture, Inc.
대리인 / 주소
Fenwick & West LLP
인용정보
피인용 횟수 :
0인용 특허 :
44
초록
Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.
대표청구항▼
1. A synthetic combination comprising a purified microbial population in association with a plurality of seeds or seedlings of an agricultural plant, wherein the purified microbial population comprises a first endophyte capable of production of acetoin, wherein the first endophyte is of the genus Cl
1. A synthetic combination comprising a purified microbial population in association with a plurality of seeds or seedlings of an agricultural plant, wherein the purified microbial population comprises a first endophyte capable of production of acetoin, wherein the first endophyte is of the genus Cladosporium and comprises an ITS rRNA nucleic acid sequence comprising SEQ ID NO: 3694, and wherein the first endophyte is present in the synthetic combination in an amount effective to provide a benefit to the seeds or seedlings or the plants derived from the seeds or seedlings. 2. The synthetic combination of claim 1, wherein the first endophyte is localized on the surface of the seeds or seedlings. 3. The synthetic combination of claim 1, wherein the first endophyte is obtained from a plant species other than the seeds or seedlings of the synthetic combination. 4. The synthetic combination of claim 1, wherein the benefit is selected from the group consisting of increased root biomass, increased root length, increased height, increased shoot length, increased leaf number, increased water use efficiency, increased overall biomass, increase grain yield, increased photosynthesis rate, increased tolerance to drought, increased heat tolerance, increased salt tolerance, increased resistance to nematode stress, increased resistance to a fungal pathogen, increased resistance to a bacterial pathogen, increased resistance to a viral pathogen, a detectable modulation in the level of a metabolite, and a detectable modulation in the proteome relative to a reference plant. 5. The synthetic combination of claim 1, wherein the combination comprises seeds and the first endophyte is associated with the seeds as a coating on the surface of the seeds. 6. The synthetic combination of claim 1, wherein the combination comprises seedlings and the first endophyte is contacted with the seedlings as a spray applied to one or more leaves and/or one or more roots of the seedlings. 7. The synthetic combination of claim 1 comprising a plurality of seeds, wherein the first endophytes are present in an amount of at least about 100 CFU or spores, at least 300 CFU or spores, at least 1,000 CFU or spores, at least 3,000 CFU or spores, at least 10,000 CFU or spores, at least 30,000 CFU or spores, at least 100,000 CFU or spores, at least 300,000 CFU or spores, or at least 1,000,000 CFU spores per seed. 8. A plurality of synthetic combinations of claim 1, placed in a medium that promotes plant growth, said medium selected from the group consisting of: soil, hydroponic apparatus, and artificial growth medium. 9. A plant grown from the synthetic combination of claim 1, said plant exhibiting an improved phenotype of agronomic interest, selected from the group consisting of: disease resistance, drought tolerance, heat tolerance, cold tolerance, salinity tolerance, metal tolerance, herbicide tolerance, chemical tolerance, improved water use efficiency, improved nitrogen utilization, improved nitrogen fixation, pest resistance, herbivore resistance, pathogen resistance, increased yield, increased yield under water-limited conditions, health enhancement, vigor improvement, growth improvement, photosynthetic capability improvement, nutrition enhancement, altered protein content, altered oil content, increased biomass, increased shoot length, increased root length, improved root architecture, increased seed weight, altered seed carbohydrate composition, altered seed oil composition, number of pods, delayed senescence, stay-green, and altered seed protein composition. 10. An agricultural product comprising a 1000 seed weight amount of a synthetic combination of claim 1. 11. The synthetic combination of claim 1, wherein the agricultural plant is a monocot. 12. The synthetic combination of claim 11, wherein the agricultural plant is corn, wheat, barley, rice, sorghum, millet, oat, triticale, rye, bamboo, or sugarcane. 13. The synthetic combination of claim 12, wherein the agricultural plant is wheat. 14. The synthetic combination of claim 1, wherein the agricultural plant is a dicot. 15. The synthetic combination of claim 14, wherein the agricultural plant is soybean, canola, rapeseed, cotton, alfalfa, cassava, potato, tomato, pea, chick pea, lentil, flax, or pepper. 16. The synthetic combination of claim 1, wherein the first endophyte is capable of utilizing proline as a sole carbon source, the benefit is growth improvement, and the agricultural plant is wheat. 17. The synthetic combination of claim 1, wherein the first endophyte is capable of utilizing proline as a sole carbon source, the benefit is drought resistance, and the agricultural plant is soybean. 18. The synthetic combination of claim 1, further comprising a second endophyte, wherein the second endophyte is of the genus Nigrospora. 19. The synthetic combination of claim 18, where in the benefit is growth improvement, and the agricultural plant is soybean. 20. The synthetic combination of claim 18, where in the benefit is growth improvement, and the agricultural plant is wheat. 21. The synthetic combination of claim 18, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO: 3685. 22. The synthetic combination of claim 1, further comprising a second endophyte, wherein the second endophyte is of the genus Fusarium. 23. The synthetic combination of claim 22, where in the benefit is growth improvement, and the agricultural plant is soybean. 24. The synthetic combination of claim 22, where in the benefit is growth improvement, and the agricultural plant is wheat. 25. The synthetic combination of claim 22, where in the benefit is increased drought resistance, and the agricultural plant is wheat. 26. The synthetic combination of claim 22, where in the benefit is increased drought resistance, and the agricultural plant is soybean. 27. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO: 3613. 28. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO: 3692. 29. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO: 3695.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (44)
Beaujot, Patrick M., Air seeder/fertilizer apparatus having metering means and distribution manifold with selectively openable ports.
Isawa, Tsuyoshi; Yasuda, Michiko; Shinozaki, Satoshi; Nakashita, Hideo; Kudo, Toshiaki, Bacteria and method for controlling plant disease using the same.
Henson, Joan M.; Sheehan, Kathy B.; Rodriguez, Russell J.; Redman, Regina S., Curvularia strains and their use to confer stress tolerance and/or growth enhancement in plants.
Leps Walter T. (3943 - 55 Street T6L 1C1 Edmonton ; Alberta CAX) Thompson Bradley G. (39 - 3115 - 119 Street T6J 5N5 Edmonton ; Alberta CAX), Method of adhering bacteria to seed and composition therefor.
Gindrat Daniel (Bassins FL CHX) Walther Daniel (Gainesville FL), Method of protecting useful plants from diseases caused by soil-borne and seed-borne pathogens by treating seeds with cu.
Stritzker, Jochen Harald; Hill, Phil; Szalay, Aladar A.; Yu, Yong A., Methods and compositions for detection of microorganisms and cells and treatment of diseases and disorders.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Sadowski, Craig; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander, Methods of use of seed-origin endophyte populations.
Imada,Takahiro; Hiruma,Naoya; Isawa,Tsuyoshi; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Pest control method for grass family plants using endophytic bacteria, pest control material, and seed bound to the pest control material.
Hurley Richard H. (Flemington NJ) Funk ; Jr. Cyril R. (East Brunswick NJ), Plants having endophytio-fungus-enhanced performance and method of producing same.
Nielsen Sven-Erik (Trekanten 5 Hinnerup DKX) Sorensen Grete Morch (Trekanten 5 Hinnerup DKX DK-8382), Rhizobia transformants which symbiotically fixes nitrogen in non-legumes, a material for treating seeds of a non-legume.
Isawa,Tsuyoshi; Hiruma,Naoya; Imada,Takahiro; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Rice containing endophytic bacteria and method of producing it.
Gao, Ai-Guo; Kolacz, Kathryn H.; MacRae, Ted C.; Miklos, John A.; Paradise, Mark S.; Perlak, Frederick J.; Dressel Toedebusch, Andrea S., Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof.
Denisov, Gennady A.; Arehart, Alan B.; Curtin, Michael D., System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms.
David M Tricoli ; Kim J. Carney ; Paul F. Russell ; Hector D. Quemada ; Russell J. McMaster ; John F. Reynolds ; Rosaline Z. Deng, Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.