Breeding method for obtaining heterosis in lined seahorses
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01K-061/00
A01K-067/027
A01K-061/95
A01K-061/10
A01K-067/02
출원번호
15325720
(2015-12-04)
등록번호
10375937
(2019-08-13)
우선권정보
CN-2015 1 0399711 (2015-07-09)
국제출원번호
PCT/CN2015/096490
(2015-12-04)
국제공개번호
WO2017/004936
(2017-01-12)
발명자
/ 주소
Luo, Wei
Lin, Qiang
Qin, Geng
Wang, Xin
Zhang, Yanhong
Zhang, Huixian
출원인 / 주소
SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY
대리인 / 주소
JCIPRNET
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
The present invention discloses a breeding method for obtaining heterosis in lined seahorses, which comprises the following steps: S1, selecting parents of lined seahorse from populations with great differences in genetic background; S2, intensified rearing the parents before pregnancy; S3, matching
The present invention discloses a breeding method for obtaining heterosis in lined seahorses, which comprises the following steps: S1, selecting parents of lined seahorse from populations with great differences in genetic background; S2, intensified rearing the parents before pregnancy; S3, matching and breeding the parents of lined seahorse from different geographical populations according to complete diallel cross method; S4, finely nursing pregnant seahorses; S5, respectively collecting all postlarvae (filial generations) hatched by each breeding group in one week; S6, rearing the filial generations; and S7, comparing survival rate and growth performance of filial generations. The present invention, via hybridization of different geographical populations to obtain lined seahorse, makes effective use of heterosis. Survival rate and growth rate of filial generations are apparently enhanced compared to that of those filial generations without hybridization. Such method enhances genetic diversity of lined seahorses and accelerates breeding of fine strain of lined seahorses.
대표청구항▼
1. A breeding method for obtaining heterosis in lined seahorses, comprising the following steps: S1, selecting parents of lined seahorse from populations with differences in genetic background;S2, intensified rearing the parents of lined seahorse in S1;S3, completely diallel crossing, matching and b
1. A breeding method for obtaining heterosis in lined seahorses, comprising the following steps: S1, selecting parents of lined seahorse from populations with differences in genetic background;S2, intensified rearing the parents of lined seahorse in S1;S3, completely diallel crossing, matching and breeding the parents of lined seahorse from different populations in S2 according to provenance and classifying into a plurality of different breeding groups;S4, finely nursing pregnant parents of each of the different breeding groups obtained in S3 to avoid abortion;S5, respectively collecting all filial generations hatched by the pregnant parents of each of the different breeding groups in S4 in one week;S6, finely nursing the filial generations of the different breeding groups in S5 according to a feeding mode in an environment;S7, comparing survival rate and growth performance of the filial generations of the different breeding groups in S6 to obtain a strain of the lined seahorses with high survival rate, fast growth rate and good genetic diversity among the different breeding groups. 2. The breeding method according to claim 1, wherein aquaculture populations of Shenzhen Guangdong, Dongshan Fujian and Yantai Shandong are selected as the parents in S1; ages of parents are 1˜1.5 years old, weights of male parents are over 10.0 g and weights of female parents are over 8.0 g. 3. The breeding method according to claim 1, wherein the lined seahorses which are energetic, disease-free, healthy and strong, with well-developed gonad, are selected as the parents in S1, and differences of weights between all male parents or female parents are less than or equal to 2.0 g. 4. The breeding method according to claim 1, wherein specific methods for the intensified rearing described in S2 are as follows: in 1˜2 months after the parents of lined seahorse have been introduced, male and female parents are separated for feeding and are reared alone for half month; during this period, water temperature is maintained between 22˜30° C.; the parents are under intensified rearing by being fed with frozen shrimps mainly accompanied by copepods as well as artemia nauplii as baits; daily feeding amount of frozen shrimps is 5%˜8% of total weight of the lined seahorses; and there are three feedings every day which are at 8 a.m., 1 p.m. and 6 p.m. respectively. 5. The breeding method according to claim 1, wherein specific methods for the complete diallel cross of parents according to the provenance described in S3 are as follows: S31, performing matching in accordance with nine breeding groups: Yantai♀×Yantai♂, Yantai♀×Shenzhen♂, Yantai♀×Dongshan♂, Shenzhen♀×Shenzhen♂, Shenzhen♀×Yantai♂, Shenzhen♀×Dongshan♂, Dongshan♀×Dongshan♂, Dongshan♀×Yantai♂ and Dongshan♀×Shenzhen♂;S32, respectively putting the parents of each breeding group in S31 into imitating ecotype white buckets for matching and breeding; each of the imitating ecotype white bucket contains 4 pairs or more than 4 pairs of parents; each breeding group comprises 12 pairs or more than 12 pairs of parents. 6. The breeding method according to claim 5, wherein each of the imitating ecotype white bucket described in S32 contains same amount of female and male parents respectively; during operation, make sure the seahorses are handled gently and put latex gloves on; the seahorses twining together are separated gently under water in order to reduce the stimulation and harm to the seahorses as much as possible. 7. The breeding method according to claim 6, wherein after the parents are put into the imitating ecotype white buckets, stimulation of slight water flow in 0.05 m3/h is maintained. 8. The breeding method according to claim 1, wherein specific methods for the finely nursing described in S4 are as follows: temperature difference of water is no more than 3° C.; an inspection on the physical condition of the seahorse being performed out of water is no more than two minutes; daily feeding amount during pregnancy should be reduced compared to the period without pregnancy, and the seahorses are fed with frozen shrimps 3˜5 times daily while the total feeding amount is 4%˜7% of the weight of the seahorse. 9. The breeding method according to claim 1, wherein conditions and management ways of the filial generations of the different breeding groups finely nursing in S6 are substantially the same to each other, wherein the filial generations of the different breeding groups are put into buckets separately for rearing, with density being 500 seahorses/m3, and the seahorses are fed with artemia naupliis as baits mainly, and the suitable feeding amount of baits is whereby a little bit of the baits are left in water when the seahorses are fed again; when the lined seahorses are 1 month old, they are transferred to a small size concrete pond for rearing, with density being 100 seahorses/m3, and the lined seahorses are fed with artemia nauplii and frozen shrimps while daily feeding amount of frozen shrimps is 5%˜8% of total weight of the seahorses; when the lined seahorses are 2˜3 months old, they are transferred to an adult fish rearing pond for rearing, with density being 20 seahorses/m3, and the lined seahorses are fed with frozen shrimps while daily feeding amount of frozen shrimps is 5%˜8% of total weight of the seahorses. 10. The breeding method according to claim 1, wherein specific methods for the comparison of survival rate and growth performance of the filial generations described in S7 are as follows: the survival rates of the filial generations from each breeding group are compared when the lined seahorses are 1, 2 and 4 months old respectively; when the lined seahorses are 4 months old, weights of the lined seahorses from each breeding group are compared.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.