초록 ▼

□ AthMRPS 유전자의 발현 연구: Promoter-GUS 분석과 Northern blot analysis
- 뿌리, 통도조직, 공변세포, 화분에서 발현
□ AtMRP5의 생화학적 기능 규명: HEK293 cell에서 과다발현
- GFP-AtMRP5가 HEK293 cell의 원형질막에 분포
- Sulfonylurea ([³H]glibenclamide)에 고친화력 리간드-수용체 결합능 확인
□ atmrp5-2 변이체 동정 및 표현형 분석: Reverse genetics screening
1)

□ AthMRPS 유전자의 발현 연구: Promoter-GUS 분석과 Northern blot analysis
- 뿌리, 통도조직, 공변세포, 화분에서 발현
□ AtMRP5의 생화학적 기능 규명: HEK293 cell에서 과다발현
- GFP-AtMRP5가 HEK293 cell의 원형질막에 분포
- Sulfonylurea ([³H]glibenclamide)에 고친화력 리간드-수용체 결합능 확인
□ atmrp5-2 변이체 동정 및 표현형 분석: Reverse genetics screening
1) Salt stress hypersensitivity
- 100 mM NaCl에 염류특이적 과민성 표현형
- MRP inhibitior인 sulfonylurea가 돌연변이의 효과를 재현 (chemical phenocopy)
- $K^{+}$ uptake에 결함이 있음을 관찰
- K+ 영양학에 결함
-SOS1, SOS2, SOS3와 AtMRP5 유전자 발현 조사: SOS1, 2, 3의 상위에서 작용
□ 그 외 3종의 ABC 수송체 유전자의 클로닝과 발현 분석

Abstract ▼

Recently, a new member of the ABC transporter superfamily of Arabidopsis, AtMRP5, was identified and characterized. In the present work, we found that AtMRP5 can bind specifically to sulfonurea when it is expressed in HEK293 cells. We also present evidence for a new role of AtMRP5 in the salt-stress

Recently, a new member of the ABC transporter superfamily of Arabidopsis, AtMRP5, was identified and characterized. In the present work, we found that AtMRP5 can bind specifically to sulfonurea when it is expressed in HEK293 cells. We also present evidence for a new role of AtMRP5 in the salt-stress response of Arabidopsis. We used reverse genetics to identify an Arabidopsis mutant (atmrp5-2) in which the AtMRP5 gene was disrupted by T-DNA insertion. In root-bending assays using MS medium supplemented with 100 mM NaCl, root growth of atmrp5-2 was substantially inhibited in contrast to the almost normal growth of wild-type seedlings. This hypersensitive response of the atmrp5-2 mutant was not observed during mannitol treatment. The root growth of the wild-type plant grown in MS medium supplemented with the MRP inhibitor glibenclamide and NaCl was inhibited to a very similar extent as the root growth of atmrp5-2 grown in NaCl alone. The $Na^{+}$ -dependent reduction of root growth of the wild-type plant in the presence of glibenclamide was partially restored by diazoxide, a known $K^{+}$ channel opener that reverses the inhibitory effects of sulfonylureas in animal cells. Moreover, the atmrp5-2 mutant was defective in $ ^{86}Rb^{+}$ uptake. When seedlings were treated with 100 mM NaCl, atmrp5-2 seedlings accumulated less $K^{+}$ and more Na+ than those of the wild type. These observations suggest that AtMRP5 is a putative sulfonylurea receptor that is involved in $K^{+}$ homeostasis and thus also participates in the NaCl stress response.

목차 Contents

• 표지...1
• 제출문...2
• 보고서 초록...3
• 요약문...4
• SUMMARY...6
• CONTENTS...7
• 목차...8
• 제1장 연구개발과제의 개요...9
• 제1절 연구개발의 목적...9
• 제2절 연구개발의 필요성 및 범위...9
• 제2장 국내외 기술개발 현황...11
• 제3장 연구개발수행 내용 및 결과...13
• 제4장 목표달성도 및 관련분야에의 기여도...21
• 제5장 연구개발결과의 활용계획...22
• 제6장 연구개발과정에서 수집한 해외 과학기술정보...23
• 제7장 참고문헌...25