초록 ▼

1. 연구개발목표 및 내용목표 1. 저선량 (0.2Gy 이하) 에서 DNA 손상 특성을 확인할 수 있는 실험체계 수립목표 2. 방사선 손상 DNA와 내재성 손상 DNA의 유의한 차이여부 규명에 필요한 분석 기술 개발내용 1. Micronucleus counting, FISH, DNA array, RT-PCR assay, characterisation of marker gene function 등의 방법으로 저선량 방사선의 영향을 측정할 수 있는 실험 체계 확립.내용 2. 방사선 특이 손상 DNA와 내재성 손상 DNA의 차이 규명을

1. 연구개발목표 및 내용목표 1. 저선량 (0.2Gy 이하) 에서 DNA 손상 특성을 확인할 수 있는 실험체계 수립목표 2. 방사선 손상 DNA와 내재성 손상 DNA의 유의한 차이여부 규명에 필요한 분석 기술 개발내용 1. Micronucleus counting, FISH, DNA array, RT-PCR assay, characterisation of marker gene function 등의 방법으로 저선량 방사선의 영향을 측정할 수 있는 실험 체계 확립.내용 2. 방사선 특이 손상 DNA와 내재성 손상 DNA의 차이 규명을 위하여 먼저 모델시스템을 확립하였고 이를 방사선을 포함하는 여러 Stress 에 대한 영향, 지표 유전자 기능 분석 등 통하여 규명2. 연구결과-개선된 Micronucleus측정 방법, FISH 및 immunohistochemistry 방법 및 유전 표지자를 이용한 저선량 방사선에 의한 DNA 손상 실험체계 구축과 손상 실험 및 실험 체계의 신뢰도 검증.-방사선 특이 손상 DNA와 내재성 손상 DNA의 유의한 차이를 구분할 수 있는 동물 실험모델 및 in vitro 모델의 확립과 손상 DNA 차이 분석 기술 개발-방사선, mutagen 흑은 다른 damaging agents 에 의한 DNA damage 의 유의한 차이 연구3. 기대효과 및 활용방안-저선량 방사선 영향 측정 기술 활용-저선량 방사선에 대한 molecular dosimetry 개발 자료로 활용-저선량 방사선 민감 유전자의 저선량 지표로 이용-DNA손상 회복 기능 연구 자료로 활용

Abstract ▼

Although we are exposed to low-dose ionizing radiations everyday, we do not have clear understanding of the effect of low dose radiation on cellular functions. In order to clarify the uncertainty, we characterized the effect of low-dose ionizing radiation on cellular functions.As a first step, we fo

Although we are exposed to low-dose ionizing radiations everyday, we do not have clear understanding of the effect of low dose radiation on cellular functions. In order to clarify the uncertainty, we characterized the effect of low-dose ionizing radiation on cellular functions.As a first step, we focused our efforts on technology development. Since detection of cellular response by low-dose ionizing irradiation has been a major obstacle in studying low-dose radiation response, we developed various tools to detect the response. For example, we took advantage of modern molecular biology and DNA technology and developed 1) advanced micronuclei counting and FISH immunofluorescence technology 2) identification system for low-dose radiation-induced genes, and 3) in vivo and in vitro experimental system to measure DNA damage.As the next step, we utilized these methods for low dose radiation to characterize the response. Low-dose radiation response of cells revealed its uniqueness that is not apparent in that of high dose. Low-dose of ionizing radiation induced a unique set of genetic markers that are not easily observed in high dose. These genes were induced in as low as 1 cGy. This sensitive modulation of gene expression by low-dose enabled us to use the genes as markers for low-dose radiation. Low-dose induced genes were expressed in different tissues with varying degrees. Using this tissue specificity of low-dose specific marker genes, we will be able to develop a tool that can detect tissue sensitivity to radiation. Moreover, with these results, we will be able to develop a dosimetry system that measures effective biological dose rather than physical one. FISH, micronuclei technology, and genetic markers can be employed to obtain biological dosimetry.This investigation will lead to several application in basic and applied fields. Basic measurement or tools for low-dose radiation can be used in academic or research lab. setting as well as in an environment where radiation technology is usually employed.Also we believe that the technologies that we developed are the basis for a construction of databases of biological dosimetry for workers handling radiation.Future investigation of the characterization of low-dose radiation effect will be aiming at DNA damage response including DNA damage signaling, repair, bystander effect and the consequences of those activities in general.As such, the study of ''Biological characterization of low-dose radiation effect'' is and continues to be an important research activity.