보고서 정보
주관연구기관 |
네오팜(주) |
연구책임자 |
박병덕
|
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2008-04 |
과제시작연도 |
2007 |
주관부처 |
보건복지부 |
사업 관리 기관 |
한국보건산업진흥원 Korea Health Industry Development Institute |
등록번호 |
TRKO200900000023 |
과제고유번호 |
1465009355 |
사업명 |
보건의료기술 연구개발 |
DB 구축일자 |
2013-04-18
|
키워드 |
주름개선.피부노화예방.칼슘신호기전.기능성화장품.wrinkle improvement.anti-skin aging.calcium signal.functional cosmetics.
|
초록
각질형성세포와 섬유아세포에서 칼슘신호전달 조절 기능을 지닌 물질을 합성, 개발하고 이를 이용하여 피부 노화 개선 효능을 지닌 기능성 화장품 원료인 L12를 개발하였다. 특히 본 연구 과제를 통하여 개발된 L12는 섬유 아세포의 칼슘신호 전달 기전을 활성화하며, 세포 증식 촉진, 콜라겐 합성 촉진 등의 효능을 지닌 것으로 밝혀졌으며, 내인성 노화와 광노화 동물 모델에서 유의한 주름 개선 효능을 지닌 것으로 나타났다. 이와 함께, 레티놀에 비하여 피부 자극이 매우 낮은 것으로 나타났다.
Abstract
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A lot of molecules are currently used or under being investigated as anti-aging agents and diverse underlying mechanisms are suggested for each of these molecules. A lot of molecules, however, are not appropriate for practical application, because of their insufficient in vivo efficacy or side-effec
A lot of molecules are currently used or under being investigated as anti-aging agents and diverse underlying mechanisms are suggested for each of these molecules. A lot of molecules, however, are not appropriate for practical application, because of their insufficient in vivo efficacy or side-effects, such as skin irritation. Previously, it was suggested that sphingosine kinase activator may be used for anti-aging, mainly through the sphingosine-1-phosphate involved signaling. A newly synthesized molecules, K6PC-5, is a new anti-aging cosmeceutical agent, and its application in either intrinsically aged or UV-induced aged mice have been reported. As a continuation of the previous researches, K6PC-5 derivative molecules using amino acids were synthesized in this research and their anti-aging and wrinkle improving efficacy was tested in vitro and in vivo. Firstly, newly synthesized molecules are tested for their activity on calcium signaling, proliferation of cultured human fibroblast and collagen synthesis in fibroblast. In addition, effect on the terminal differentiation of human keratinocytes was also evaluated. Among the tested molecules, K6-L12 showed most significant calcium signaling in both the cultured keratinocyte and fibroblast, and also induced proliferation of fibroblast and increased collagen synthesis. In order to verify the anti-aging efficacy of K6-L12 on intrinsically aged skin, murine aged mice model was established and dermal changes were investigated. When 1% K6-L12 was topically applied twice a day for 2 wks in 56-wks aged mice skin, and functional measurements and histologic observation was performed. No changes in transepidermal water loss and skin capacitance was observed between vehicle treated and K6-L12 treated groups. However, dermal thickness was significantly increased in K6-L12 treated skin and, the increased number of fibroblast and collagen density was also observed. In a photo-aged model induced by chronic UV-irradiation, topical application of L12 also improved collagen density of dermal tissue and also skin barrier function. When topical retinol was used as a positive control, significant skin irritation was observed in retinol treated skin site, but L12 treated site did not show irritation, which suggests that L12 can be used as an anti-aging agent for sensitive skin. In order to access the potential toxicity of L12, 6 kinds of toxicity tests were performed in non-clinical CRO organization. As results, L12 did not result in any significant toxicity under tested condition, and it can be concluded that L12 is a safe cosmetic ingredient. It was also listed in the International Cosmetic Ingredient Dictionary (ICID). In order to use L12 as a cosmetic ingredient, large scale synthesis was performed and its final yield was 80%, and its cost was evaluated as HRW 101,200/kg. Currently, balm type product is under clinical investigation for verifying its anti-wrinkle efficacy. In order to develop new molecules which having skin barrier improving activity, we have screened the synthesized molecules for their effects on skin barrier function, and identified several molecules are beneficial effects on barrier function. Interestingly, the potential mediating molecules were suggested as an anti-microbial peptides (AMPs) and, using the synthesized molecules, their effects on mRNA expression of AMPs was evaluated using real-time reverse-transcriptase polymerase chain reaction (RT-PCR). As a result, L19 was identified as having stimulating effects on the AMPs mRNA expression in cultured human keratinocytes, without affecting the proinflammatory cytokines production. In a chronic dermatitis model induced by long-term oxazolone application, considerable increase of epidermal AMPs was observed in 1% L19 applied skin. These results suggest that K6-L19 may be used as a possible medication for infectious skin diseases and atopic eczematous lesions.
목차 Contents
- II. 총괄연구과제 연구결과...13
- 1. 연구개발과제의 배경 및 필요성...14
- 2. 국내외 기술개발 현황...15
- 3. 연구개발과제의 추진체계...19
- 3.1 연구개발의 추진전략.방법...19
- 3.2 연구개발의 추진체계...20
- 3.3 연구개발기관의 구성체계...21
- 4. 연구개발수행 내용 및 결과...22
- 4.1 소재의 합성...22
- 4.2 소재 평가 방법...32
- 4.3 in vitro 평가 결과...35
- 4.4 in vivo 평가...45
- 4.5 최종 선정 소재의 원가 및 최종 수율...58
- 4.6 최종 선정 소재의 화장품 원료화...63
- 4.7 최종 선정 소재를 이용한 화장품 제형의 개발...70
- 4.8 최종 선정 소재의 제품화 결과...73
- 5. 목표달성도 및 관련분야 기여도...74
- 5.1 총괄연구개발과제의 목표달성도...74
- 5.2 관련분야 기여도...75
- 6. 향후 연구성과 추진 계획...76
- 7. 연구개발결과의 파급효과...79
- 7.1 기술적 파급효과...79
- 7.2 경제.산업적 파급효과...79
- 7.3 사회적 파급효과...79
- 8. 연구개발결과의 활용계획...80
- 8.1 연구개발결과의 활용계획 및 추가연구의 필요성...80
- 9. 연구개발과정에서 수집한 해외과학기술정보...82
- 10. 참고문헌...82
- IV. 첨부서류...84
- 1. 자체평가의견서...85
- 2. 실적 증빙서류...89
- 3. 기타 첨부서류...89
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