초록

1)TRP 이온통로의 미각 기관에서의 발현양상을 조사하고 TRP 이온통로 돌연변이 초파리의 맛 인지 능력을 행동학적, 전기 생리학적으로 분석
2) 분자 유전학적 기법을 통하여 특정 초파리 미각 수용체 돌연변이 초파리를 만들고 미각 수용체 돌연변이 초파리를 이용하여 미각 수용체의 효현제을 찾음.

Abstract ▼

Taste is very essential sensory modality. So we believe that the taste transduction mechanism might be conserved throughout the animal kingdom. In this study, we tried to increase our understanding to Drosophila taste transduction and test the hypothesis that fly can be a model system to study human

Taste is very essential sensory modality. So we believe that the taste transduction mechanism might be conserved throughout the animal kingdom. In this study, we tried to increase our understanding to Drosophila taste transduction and test the hypothesis that fly can be a model system to study human taste. We found which TRP channels are expressed in the fly taste organs. Among them we studied a greater detail in the expression and function of pyrexia. Pyrexia is expressed in the accessory cells instead of gustatory receptor neurons. It seems that pyrexia is not indispensible for taste transduction. We also characterized the function of GR33a. We generated Gr33a missing flies and tested their taste function. Gr33a mutant flies were impaired in the avoidance responses to all non-volatile repellents tested, ranging from quinine to denatorium, lobeline and caffeine. Gr33a mutant males also displayed increased male-to-male courtship implying that it functioned in the detection of a repulsive male pheromone. GR33a may function as co-receptor required for sensing all non-volatile repulsive chemicals, including tastants and pheromones. We need to investigate whether other TRPs are involved in Drosophila taste transduction. GR33a is a molecular target to control disease vectors and pest. It is very intriguing that GR33a inhibitor can help insects take insecticide without sensing bitterness.

목차 Contents

• 표지 ...1
• 제출문 ...3
• 목차 ...4
• Ⅰ. 총괄현황 ...5
• 보고서 요약서 ...6
• 요약문 ...7
• SUMMARY ...8
• 연구 성과 실적 및 향후 계획 ...9
• 참여연구원 현황표 ...12
• Ⅱ. 총괄연구과제 연구결과 ...13
• 1. 연구개발과제의 배경 및 필요성 ...14
• 2. 국내외 기술개발 현황 ...18
• 3. 연구개발과제의 추진체계 ...20
• 4. 연구개발수행 내용 및 결과 ...22
• 5. 목표달성도 및 관련분야 기여도 ...34
• 6. 향후 연구성과 추진 계획 ...35
• 7. 연구개발결과의 파급효과 ...35
• 8. 연구개발결과의 활용계획 ...37
• 9. 연구개발과정에서 수집한 해외과학기술정보 ...37
• 10. 참고문헌 ...37
• Ⅲ. 첨부서류 ...39
• 실적증빙자료 및 첨부서류 목차 ...40
• 1. 자체평가의견서 ...41
• Ⅰ. 연구개발실적 ...42
• Ⅱ. 연구목표 달성도 ...43
• Ⅲ. 종합의견 ...43
• Ⅳ. 보안성 검토 ...43
• 2. 실적증빙자료 ...44
• 3. 기타 첨부서류 ...44