- 기존 질소제거공정에 비해 획기적을 에너지를 절감할 수 있고, 고속으로 질소를 제거할 수 있는 혐기성 암모늄 산화균의 고농도 배양기술을 확보, 입상화 기술과 고율 질소제거 시스템을 개발. - 부직포와 혐기성 입상 슬러지를 이용하여 반응기내 미생물의 농도를 15g/L이상 유지할 수 있었고, 생물학적 입상화와 화학적 입상화를 성공적으로 수행하여, 2 kg/$m^{3}$-day(기존 질소제거공정의 5배이상)이상의 속도로 안정적으로 질소제거 달성. - FISH (Fluorescence In Situ Hybr
- 기존 질소제거공정에 비해 획기적을 에너지를 절감할 수 있고, 고속으로 질소를 제거할 수 있는 혐기성 암모늄 산화균의 고농도 배양기술을 확보, 입상화 기술과 고율 질소제거 시스템을 개발. - 부직포와 혐기성 입상 슬러지를 이용하여 반응기내 미생물의 농도를 15g/L이상 유지할 수 있었고, 생물학적 입상화와 화학적 입상화를 성공적으로 수행하여, 2 kg/$m^{3}$-day(기존 질소제거공정의 5배이상)이상의 속도로 안정적으로 질소제거 달성. - FISH (Fluorescence In Situ Hybridization)를 이용하여 반응기내 혐기성 암모늄 산화균이 50%이상 우점하는 것을 확인. - 분자생물학적 기법을 이용하여 배양이 불가능한 혐기성 암모늄산화균의 군집특성을 조사한 결과 혐기성 암모늄산화균 및 Chloroflexi를 비롯한 다양한 미생물의 군집특성을 밝힘. - 입상슬러지와 부직포를 결합한 복합형 반응기를 이용하여 30일이내에 0.4 kgN/$m^{3}$-day의 질소제거가 가능하였고, 하수처리장 혐기성소화조 탈리액을 대상으로 적용성시험을 수행한 결과 80%이상의 우수한 질소제거특성을 나타내었음.
Abstract▼
This research conducted for 2 years and 10 months (2004. 6. 1-2007. 3. 31), consisted of two main parts. Firstly, process evaluation with engineering point of view was focused on incubation of highly concentrated anammox bacteria, granulation of anammox bacteria and system development for high-rate
This research conducted for 2 years and 10 months (2004. 6. 1-2007. 3. 31), consisted of two main parts. Firstly, process evaluation with engineering point of view was focused on incubation of highly concentrated anammox bacteria, granulation of anammox bacteria and system development for high-rate nitrogen removal. Secondly, microbial analysis by means of molecular biological techniques was taken to prove microbial community in granular sludge and the predominating species. Moreover, the applicability was evaluated on reject water from anaerobic digester in wastewater treatment plant. In the $1^{st}$-year research, anammox bacteria were screened and cultured from various wastewater such as reject water, leachate, urine and livestock wastewater containing high ammonia nitrogen concentration. Optimum operational parameters were investigated through anaerobic batch tests applied to synthetic wastewater. It was also tried to obtain the fundamental technique to cultivate highly concentrated anammox bacteria in fed-batch operation by stimulating growth rate. In the $2^{nd}$-year research, based on the $1^{st}$-year results (DO concentration, influent nitrogen concentration, reducing agent addition, hydrazine addition, etc), anammox bacteria were cultivated in UASB reactor to examine the optimum conditions in continuous operation. To get over relatively long doubling time(about 11 days) of anammox bacteria, granular type of anammox bacteria formed biologically and chemically were used to estimate whether its activity increased or not. The continuous reactor was designed to maintain long SRT, more than 100 days, counterbalancing slow growth rate, and on-line real time monitoring system was equipped to control operational parameters (pH, ORP, temperature) which was attributed to set up stable nitrogen removal system. In the $3^{rd}$-year research, the ability to remove nitrogen was evaluated when applied to wastewater containing high nitrogen concentration(ammonium nitrogen 500 mg/L, nitrite nitrogen 500 mg/L) in bioreactor designed to have SRT more than 100 days. The techniques acquired from previous research were also used to cultivate and granulate anammox bacteria continuously in order to retain anammox bacteria. Additionally, the supernatant generated after anaerobic digestion of sludges (recycle water) was employed as a target to test the adaptability of anammox bacteria in the real wastewater. The activity of anammox bacteria from the continuous reactor was evaluated by RT-PCR, the quantitative analysis method. Fluorescent in situ hybridization (FISH), appropriate technique to analyze bacteria which are impossible to get in pure culture, was also applied to observe distribution characteristics. The gene probe developed previously as a oligonucleotide type was used in detecting anammox bacteria.
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