초록 ▼

제1세부과제에서는 탄수화물 소재 기능성 성분으로 알려진 phytate, β-glucan, 올리고당류의 분석 방법을 확립하였으며, 기능성 성분표 작성 및 생리활성 성분의 적정 섭취량 설정 연구의 기초자료로써 두류 및 곡류 등 24종의 우리 농산물에 대한 phytate, β-glucan, 올리고당류의 함량을 정량 분석하였음. 또한, 동물실험을 통하여 국내 농산물에 함유된 phytate의 지질대사 및 무기질 대사에 미치는 영향을 조사하여 유의적 효과가 있음을 밝혔으며 도토리 전분이 당뇨 마우스의 혈당이 및 당화혈색소 수준을 낮추고 내당능

제1세부과제에서는 탄수화물 소재 기능성 성분으로 알려진 phytate, β-glucan, 올리고당류의 분석 방법을 확립하였으며, 기능성 성분표 작성 및 생리활성 성분의 적정 섭취량 설정 연구의 기초자료로써 두류 및 곡류 등 24종의 우리 농산물에 대한 phytate, β-glucan, 올리고당류의 함량을 정량 분석하였음. 또한, 동물실험을 통하여 국내 농산물에 함유된 phytate의 지질대사 및 무기질 대사에 미치는 영향을 조사하여 유의적 효과가 있음을 밝혔으며 도토리 전분이 당뇨 마우스의 혈당이 및 당화혈색소 수준을 낮추고 내당능을 개선시킴을 확인하였다. 제1협동과제는 Phytochemicals 중 기능성 성분 분석 및 생리활성 구명 연구로 isoflavones, flavanones 및 anthocyanins 성분 분석방법을 확립하였으며, 두류 및 곡류 등 우리 농산물에 대한 isoflavones 성분 33품목 및 anthocyanins 성분 17품목을 분석하여 기능성 성분표 작성 및 생리활성 성분의 적정 섭취량 연구의 기초자료를 제공하였음. 또한, 동물실험을 통한 유색미의 기능성을 밝혀 우리 쌀의 우수성을 입증하였음. 제2협동과제는 특수미량성분 빛 단백질 소재 기능성 성분의 분석 연구로 국내 유통 중인 국내산 농산물 총 35점의 특수미량성분, 아미노산 및 비타민 E를 모니터링한 결과 특수미량성분의 재현성 빛 회수율은 Ge, Se, Mn 및 Zn의 재현성과 회수율 모두 95% 이상 이었다. 미량성분 중 Ge와 Se는 불검출되었으며, Mn의 경우 곡류 및 두류는 각각 0.14~3.57 mg%, 채소류 0.08~1. 55 mg%이었으며, Zn의 경우 곡류 및 두류는 0.06~3.00 mg%, 채소류는 불검출, 버섯류는 0.15~2.8 mg%이었다. 아미노산류는 평균 함량이 곡류 8, 314 mg%,, 두류 34,229 mg%, 채소류 10,337.8 mg%, 견과 및 종실류 16,887.0 mg%, 버섯류 21,772.2 mg%, 과실류 1,542.9 mg%이었다. 비타민 E는 옥수수류, 두류, 견과 및 종실류는 불검출되었으며, 채소류는 100g 당 평균 895.7 ㎍, 버섯류는 100g 당 12,142.7 ㎍, 과실류는 100g 당 평균 116.2±78.9 ㎍이었다. 제3협동과저l에서는 동물성 및 식물성 단백질유래 펩티드의 골대사 생리활성 구명 및 적정 섭취수준에 관한 연구를 수행한 결과 우유 유래 casein phosphopopeptide(CPP)가 골다공증 모델인 난소절제 흰쥐에서 체내 칼슘 및 인의 생체유용성을 증가시켰고, 무기질 축적을 증가시켰으며, 유청 유래 펩티드는 골대사기능 활성에는 유용하지 않은 것으로 나타났음. 대두펩티드는 자발성 고혈압 SHR종 쥐에서 섭취수준이 증가할 수록 Na 장관내 흡수억제, Na배설 증가, angiotensin converting enzyme 활성을 억제하므로서 혈압을 저하시켜 고혈압에 유의한 효과를 나타내었다.

Abstract ▼

Item 1: Analysis and functions of bio-active components in carbohydrate materials
This study determined phytate, β-glucan, and oligosaccharide, naturally occurring organic compounds found in plant seeds, roots, and tubers, in the Korean agricultural products using the various methods. Phytate in

Item 1: Analysis and functions of bio-active components in carbohydrate materials
This study determined phytate, β-glucan, and oligosaccharide, naturally occurring organic compounds found in plant seeds, roots, and tubers, in the Korean agricultural products using the various methods. Phytate in a sample extracts first separated and concentrated by anion-exchange chromatography and the phytate concentrate is then quantitatively determined as phosphorous by acid digestion or using inductively coupled plasma atomic emission spectrometry (ICP-AES). Also, samples were run through an anion-exchange column and total phytate content of the eluates was spectrometrically measured with the modified Wade reagent as the method of Fruhbeck et al., A little difference in phytate content was found among the methods, β-Glucan was determined according to the official AOAC method. Total sugar content was measured by phenol-sulfuric acid method using D-glucose as a standard. The oligosaccharide contents was extracted in shaking the samples added wi th water and 85% ethanol at RT for overnight and in heating the samples added with water. The extracts were analyzed by HPLC. HPLC conditions were as following: column, High Performance Carbohydrate Column (4.6 ×250 mm, Waters co.): column temperature, 35 ℃: detection, Refractive index (Waters Model 410) mobile phase, 75% acetonitrile flow rate, 1.2 ml/min. Among representive samples of 24 Korean agricultural products, the phytate content is the highest in black sesame. In cereals, phytate content expressed on a dry weight basis ranged from 1.76 mg/g in colored rice (Heugjinju, well-polished rice) to 16.48 mg/g in Paddy rice (I1pum, brown rice). In all methods, the brown rice is higher content about 2~4 times than the well-polished rice. β-glucan contents of domestic agricultural products is measured using of mixed-Linkage β-glucan kit (McCLEARY Method) ccording to the official AOAC method. Content of β-glucan was 4. 57±0.069 %w/w in Chokang barley, 0.86±0.022 %w/w in OkJeon rye. The highest content of β-glucan showed that white soybeans was 0.07±0.032 %w/w among the pulses and Daekang peanut was 0.17±0.002% w/w among nuts & seeds. β-glucan contents of cabbage and red-pepper were lower level at 0.35±0.089 %w/w, 0.36±0.146 %w/w, respectively. Content of oyster mushroom and Lentinus edodes was 0.28±0.000 %w/w and 0.27±0.018 %w/w, respectively. The analytical method for oligosaccharides using HPLC could be adopted as the quantitative analysis of the domestic agricultural products. The analytical results showed that oligosaccharides were identified to be the various kinds such as sucrose, maltose, maltotriose, raffinose, stachyose according to various extraction solvents and methods. Most of the experimental ago-products contained sucrose and the contents were 03~1.8% in rices, 0.2~20.9% in corns and 1.2~5.6% in beans. Especially, beans had the highest amount of raffinose and stachyose. The supplementary effect of phytic acid on the lipid levels in serum and liver of diabetic mice were investigated. Forty male diabetic KK mice were fed wi th purified diets supplemented with 0 (P0), 0.5 (P5), 1.0 (P10), and 1.5 % (P15) sodium phytate for 8 weeks. Diet intake, body and organ weight, and contents of serum and hepatic lipid profiles were measured. There were no signi ficant di fferences in diet intake, body weight and organ weight among experimental groups. The concentrations of serum total cholesterol and LDL-cholesterol were lower in the groups fed with phytate diets than P0, and the significance was found only in PI0 and P15. HDL-cholesterollevel of P15 was the highest among the experimental groups. The contents of hepatic total lipid and total cholesterol were lower in the groups fed wi th phytate diets. These results suggested that phytic acid affect the lipid levels in serum and liver of diabetic KK mice. Although physiological functionality is investigated in many studies, there is a few studies about adequate intake level of functional components. Therefore it is necessary to establish minimum intake levels having bioactivity of functional component from domestic agricultural products and safety levels having no effects on metabolism of other nutrients.
Cooperrative Item 1 : Analysis of functional components and evaluation of their functional properties of phytochemicals in vegetables and crops from domestic markets
This study was carried out to contribute for database establishment of functional ingredients in vegetables and crops from local markets. At the same time, two animal studies were conducted to demonstrate functional properties such as antioxidant capability and choleserol lowering effects of the phytochemicals in locally developed colored rices. Isoflavone contents in 22 legumes including imported beans from China, U. S. and North Korea, and in 11 processed legume products such as soybean sprouts (3 brands), tofu (4 brands) and soy milk (4 brands) were also determined by HPCL methods. Anthocyanin contents were measured from 15 legumes and three domestic breeds of colored rice. Anthocyanin in fruits such as grape ( 3 breeds), tangerines ( 3 breeds) and persimmon (4 breeds) were also analyzed. Flavonol (rutin, quercetin, myricetin and quercitrin) contents in buckwheat (3 breeds), buckwheat noodles (2 brands), buckwheat jelly (2 brands) were determined by a HPLC method. Capsaicin and dihyrocapsaicin contents in nine -breeds of dried hot peppers, and all in and allicin contents in three garlic breeds including one from China, three onion breeds and three green onion breeds were analyzed. Two in vivo animal studies were carried out to compare the antioxdative ability of the three colored rice breeds. The differences in experimental designs between the two studies were the levels (40 and 60% for the experiment 1 and 2, respectively) of the rice added to the diets at the expense of glucose and the means to accelerate physiological stresses. Cholesterol supplementation (0.5%) or removal of dietary vitamin E were used for stress purposes in experiments 1 or 2, respectively. There were not definitive antioxidative effects in both studies in levels of blood free radicals, catalase, liver TBARS, SOD, glutathione reductase in rats fed the colored rice. However, the diets added with jeogjinju rice tended to show lowered TBARS contents and plasma vitamin E sparing effects. Further studies are required to show relationships between the intake of functional ingredients in local vegetables and demonstration of in vivo functional activities of the ingredients.
Cooperrative Item 2 : Analysis of Functional component in protein materials and specific trace components
The research results of analysis the trace metal, amino acid and vitamin E using ICP-AES and HPLC in domestic agricultural produced foods as follows. The recoveries of Ge, Se, Mn and Zn were 96 to 98 % and repeatabilities of them were 95 to 98 %. The monitoring results showed that the trace metal contents in 35 domestic agricultural produced foods were ND in Ge, ND in Se, 0.08 to 3.57 mg% in Mn, ND to 3.00 mg% in Zn. The monitoring results showed that the amino acid contents in 30 domestic agricultural produced foods were 968.8 to 27,369. 4 mg% in total amino acid. The monitoring results showed that the vitamin E contents in 30 domestic agricultural produced foods of 30 were ND to 12.1 mg%. It is recommended that the analytical method for trace metal, amino acid and vitamin E using ICP-AES and HPLC could be adopted as the quantitative analysis to control the domestic agricultural produced foods.
Cooperrative Item 3 : Bioactive effects of the intake level of peptides from animal and vegetable protein such as milk and soy
This study evaluated the bioacti ve effects of the intake level of peptides from animal and vegetable protein such as milk and soy. In 1st year, the effect of the level of casein phosphopeptide(CPP) on mineral(Ca and P) bioavailabilties and bone biomarker of aged ovariectomized (OVX). The urinary Ca and P excretion were increased by CPP intake in OVX rats. The fecal excretion of P given CPP decreased in OVX with dose dependent manner. Ca and P contents of femur significantly increased by adding 2 or 3% of CPP when compared with OC group and OL group(p<0.05). There were no significant differences in serum alkaline phosphatase activity and c-terminal telopeptide excretion in experimental groups. Although ovariectomy induced the increase in urinary c-terminal telopeptide excretion, 2 or 3% of CPP in the diet decreased urinary c-terminal telopetide eicretion significantly. These finding suggest the usefulness of CPP in the prevention of postmenopausal bone loss by decreasing urinary Ca excretion and bone resorption. Over 2 percent of CPP in the diet was effective to prevent postmenopausal bone loss. In 2nd year, the effects of caseinomacropeptide(CMP) from whey protein and soypeptide from soy hydrolysate were studied. When growing rats were fed diet containing CMP at doses of 5%, 10%, 15%, CMP decreased food intakes, calcium and phosphorus bioavailabilities, and their contents in liver and femoral bones. Therefore, it suggests that the addtion of CMP in functional food for mineral utilization will ameliorate the mineral bioavailabitities. In other study spontaneously hypertensive SHR rats were divided into four groups that were given for 4 weeks the following diet:0%, 1%, 3%, 5% of soypeptide. Systolic blood pressure values were significantly decrease by soypeptide intakes in dose dependent manner. Apparent absorption of Na was decreased rats and urinary Na excretion increased in soy peptide treated SHR. There was a significant dose effects of soypeptide on apparent Na absorption and urinary excretion. The angiotensin converting enzyme activities were decreased by the supplementation of soypeptide. These results suggest that the soypeptide could be used as a functional food with antihypertensive activity. Therefore, these studies suggests that caseinophosphopeptide could be used as bioactive components in funtional foods for increasing mineral utilization to prevent osteoporosis and soypeptide to prevent hypertension.

목차 Contents

• 표지...1
• 제출문...3
• 요약문...5
• SUMMARY...13
• CONTENTS...19
• 목차...20
• 제 1 장 서론...21
• 제 2 장 국내외 기술개발 현황...23
• 제 1 절 국외...23
• 제 2 절 국내...25
• 제 3 장 연구개발 수행 내용 및 결과...28
• 제 1 절 탄수화물 소재 유래 기능성 성분 분석 및 생리활성 구명 연구...28
• 제 2 절 Phytochemicals 중 기능성 성분 분석 및 생리활성 구면 연구...56
• 제 3 절 특수미량성분 및 단백질 소재 기능성 성분의 분석...85
• 제 4 절 기능성 펩티드의 골대사 활성효과 구명 및 적정 섭취량 설정...103
• 제 4 장 연구개발목표 달성도 및 대외기여도...124
• 제 5 장 연구개발결과의 활용계획...126
• 제 6 장 기타 중요 변동사항...127
• 제 7 장 참고문헌...128
• 최종보고서 초록...137