보고서 정보
주관연구기관 |
국립농업과학원 |
연구책임자 |
이수형
|
참여연구자 |
예완해
,
류재기
,
이데레사
,
박경훈
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2012-02 |
과제시작연도 |
2011 |
주관부처 |
농촌진흥청 |
사업 관리 기관 |
농촌진흥청 Rural Development Administration |
등록번호 |
TRKO201200009704 |
과제고유번호 |
1395022355 |
DB 구축일자 |
2013-05-20
|
초록
쌀, 보리, 옥수수 등 주요 곡류에 대하여 푸사리움속 곰팡이의 발생생태를 조사하고, 분리균주의 병원성 검정과 기주별 우점균계를 구명하였고, 이를 통한 오염가능한 주요 독소를 분석하였다. 미곡종합처리장의 쌀 및 부산물과 보리 및 시중 유통 보리쌀, 옥수수에 대하여 데옥시니발레놀, 니발레놀, 제랄레논, 푸모니신 등 주요 곰팡이독소를 조사하였고, 우리나라 실정에 맞는 맥류에 대한 농산물우수관리기준을 개발하였다.
Abstract
▼
Fusarium head blight(FHB) caused by Fusarium graminearum is a serious disease that affects the quality and yield of cereal grain in the world. The most common fusarium mycotoxins of barley and cereal diseased by fusarium graminearum are deoxynivalenol(DON), nivalenol(NIV), and Zeralenone(ZEA). Mycot
Fusarium head blight(FHB) caused by Fusarium graminearum is a serious disease that affects the quality and yield of cereal grain in the world. The most common fusarium mycotoxins of barley and cereal diseased by fusarium graminearum are deoxynivalenol(DON), nivalenol(NIV), and Zeralenone(ZEA). Mycotoxins such as DON, NIV, and ZEN are hazardous to human and animal health. To investigate Fusarium mycotoxin contamination in stored grains, rice samples including rice-milling products such as rice husks, brown rice, blue-tinged rice, discolored rice, half-crushed rice, polished rice and rice bran were collected from rice processing complexes (RPC) nationwide. Analysis of fungal flora associated with each rice sample showed that unhusked rice was the most severely contaminated with fungi while polished rice was the least contaminated. Among others, blue-tinged rice was the most frequently contaminated with F. graminearum (3.8%), followed by discolored rice (2.4%) and brown rice (1.6%) in 2009. In 2010, the samples showed a similar contamination pattern to that of 2009. The major contaminants shown more than 5% of total fungal isolates were Aspergillus (18.4%), Fusarium (8.5%), Alternaria (6.2%), Dreschlera (5.7%), and Penicillium spp. (5.5%). In addition, more than 30 different known and unknown fungal genus were detected. During 3 years of study, we monitored grain crops such as rice, barley, wheat, and corn for Fusarium contamination. The results showed both total fungi and Fusarium contamination were affected by weather condition. In 2010, head blight occurred severely in most of the crops investigated due to more frequent rainfalls and cooler season than previous years during crop growth. In 2011, humidier weather kept during flowering stage of wheat and barley caused even more severe outbreak of head blight on these crops and great economic loss. Mycotoxin was most severely contaminated in 2010 also. In rice and its by-products samples blue-tinged rice and discoloured rice was most severely contaminated by mycotoxins. In three mycotoxins NIV was most severely contaminated. But polished rice and brown rice is safe. In 2010 barley was contaminated by DON(15%, 0.01~ 3.21 mg/kg), NIV(84.9%, 0.11~9.74 mg/kg), and ZEA(15%, 0.01~3.21 mg/kg). In 2010 corn for seed was contaminated by DON(50%, 0.03~1.35 mg/kg), NIV(79%, 0.04~4.69 mg/kg), ZEA(21%, 0.12~1.20 mg/kg), and Fumonisine(B1+B2)(93%, 0.07~9.55 mg/kg). Several countries including EU, Japan, and US have established legislative limits for DON and ZEA in cereals and cereal based products. Also, they have published Good Agricultural Practice (GAP) guideline that is a cardinal principle to reduce mycotoxins in cereal and cereal products. GAP guideline to reduce the risk of fusarium mycotoxins such as DON, NIV, and ZEA includes to avoid maize as previous crop, to minimize crop debris on soil surface, timely chemical spray application against FHB, and timely harvest in the field. It is important for producers to recognize that good agricultural practice means the guideline of prevention against mycotoxin contamination of cereals. The purpose of this study was to make an GAP guideline to reduce fusarium mycotoxin risk and fusarium head blight occurrence in barley. This GAP guideline recommends growers how they can prevent the risk of fusarium mycotoxins by applying their cultivation.
목차 Contents
- 표지 ... 1
- 제출문 ... 2
- 요약문 ... 3
- SUMMARY ... 5
- 제1장 서 론 ... 7
- 제1절 연구개발의 목적 ... 7
- 제2절 필요성 및 범위 ... 7
- 제2장 국내외 기술개발 현황 ... 9
- 제1절 국·내외 관련분야에 대한 기술개발현황 ... 9
- 제2절 연구결과가 국·내외 기술개발현황에서 차지하는 위치 ... 10
- 제3장 연구개발수행 내용 및 결과 ... 12
- 제1절 2009년도 곡류 곰팡이독소 오염예방 GAP 가이드라인 설정연구 ... 12
- 제2절 2010년도 곡류 Fusarium 곰팡이독소 안전관리 기반기술 개발 ... 42
- 제3절 2011년도 곡류 Fusarium 곰팡이독소 안전관리 기반기술 개발 ... 66
- 제4장 연구개발목표 달성도 및 대외기여도 ... 82
- 제1절 목표대비 달성도 ... 82
- 제2절 정량적 성과 ... 83
- 제5장 연구개발결과의 활용계획 ... 86
- 제6장 중요 변동사항 ... 87
- 제7장 참고문헌 ... 88
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