초록

Ⅲ. 연구개발의 내용 및 범위
○ 친환경 작물보호용 고활성 미생물과 천연 활성물질선발 및 담체화 기술을 이용한 기능 향상

Abstract ▼

In the present study, encapsulation of phosphonic acid and some essential oil with nano silicate were used for the protection of chili-pepper plants from Phytophthora blight caused by Phytophthora capsici and other major plant pathogens. The microporous silica matrix was used as a carrier for the co

In the present study, encapsulation of phosphonic acid and some essential oil with nano silicate were used for the protection of chili-pepper plants from Phytophthora blight caused by Phytophthora capsici and other major plant pathogens. The microporous silica matrix was used as a carrier for the controlled release of potassium phosphonic acid and essential oils. Encapsulated phosphonic acid and essential oils showed strong activity for plant growth promotion and disease control which based on slow releasing effect of active ingradients. A strain of plant growth-promoting fungi was isolated from rhizosphere of wild plants showed plant growth promotion and induced systemic resistance (ISR) on tobacco plants. From the results of I-Plate assay, tobacco plants showed significant plant growth promotion by unknown volatiles of a plant growth promoting-fungi, Cladosporium sp. CL-1. Furthermore, when the isolate inoculated on the roots of tobacco, plant showed ISR against Pectobacterium carotovora SCC1. The results suggest that volatiles from the strain of CL-1 involve plant growth promotion as well as ISR. These results clearly indicate that the encapsulated metabolites is one of the effective methods for the control of plant pathogens such as Phytophthora blight and Pectobacterium carotovora on chili-pepper plants when compared to individual application. Methanol at 60~80% fractions from antibiotic Bacillus strains, 77 against fusarium disease showed antifungal activity, whereas, MeOH at 100% showed ISR activity. A selected PGPR strain Pseudomonas fluorescens SS101 producing crude CLP showed plant protection activities against biotic and abiotic stress.
In the study of conducted to develop biocontrol agents against white rot accused by Sclereotium cepivorum in Allium spp. Micororganisms were isolated from the soils and sclerotia of Sclerotium cepivorum and Sclerotinia sclerotiorum. Three isolates of fungi were identified as Trichoderma virens Tr1047, T. harzianum Tr1048, and Paraconiothyrium minitans S134 by morphological, molecular and cultural characteristics. All isolates tested against sclerotia of Sclereotium cepivorum. In the
field test, A mycoparasite, Paraconiothyrium minitans S134 was selected as biological control agents against S. cepivorum. The experiment was carried out in a garlic field in Taean from October 2011 to June 2012. Spore suspensions of the mycoparasite was treated twice onto soil surface around garlic plants in grown in 2011 and late February 2012, and disease rating was recorded in June 2012. Incidence of white twice after the application, the plot of the P. minitans S134, CM2 (5×10⁶spores/㎖) in fluquinconazole(WP)-treated plot were 6.8%, 8.6% and 0.4%, and Trichoderma virens Tr1047 and T. harzianum Tr1048 (5×10⁶spores/㎖)-treated plot were 15.8% and 21.7%, respectively, while control was 19.5%. As the results, P. minitans S134 could be prospective biofungicide for biological control of white rot of garlic.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 6
• 목차 ... 10
• 제1장 서 론 ... 11
• 제1절 나노기술 활용 생육촉진 및 유도저항성 활용 기술개발 ... 11
• 제2절 기생성곰팡이를 활용한 흑색썩음균핵병 생물적 방제 기술개발 ... 12
• 제3절 쿼름퀸칭 시스템을 활용한 식물세균병 억제기술개발 ... 13
• 제4절 유용균을 활용한 친환경 벼 종자소독제 개발 ... 13
• 제5절 벼 키다리병의 친환경 방제기술 개발 ... 14
• 제6절 유용균 및 나노기술을 이용한 뿌리혹선충 방제 기술개발 ... 14
• 제7절 천연물을 이용한 선충방제용 compost 개발 ... 15
• 제2장 국내외 기술개발 현황 ... 17
• 제1절 나노기술 활용 생육촉진 및 유도저항성 활용 기술개발 ... 17
• 제2절 기생성곰팡이를 활용한 흑색썩음균핵병 생물적 방제 기술개발 ... 17
• 제3절 쿼름퀸칭 시스템을 활용한 식물세균병 억제기술개발 ... 19
• 제4절 유용균을 활용한 친환경 벼 종자소독제 개발 ... 19
• 제5절 벼 키다리병의 친환경 방제기술 개발 ... 19
• 제6절 유용균 및 나노기술을 이용한 뿌리혹선충 방제 기술개발 ... 20
• 제7절 천연물을 이용한 선충방제용 compost 개발 ... 21
• 제3장 연구개발수행 내용 및 결과 ... 23
• 제1절 나노기술 활용 생육촉진 및 유도저항성 활용 기술개발 ... 23
• 제2절 기생성곰팡이를 활용한 흑색썩음균핵병 생물적 방제 기술개발 ... 51
• 제3절 쿼름퀸칭 시스템을 활용한 식물세균병 억제기술개발 ... 77
• 제4절 유용균을 활용한 친환경 벼 종자소독제 개발 ... 80
• 제5절 벼 키다리병의 친환경 방제기술 개발 ... 88
• 제6절 유용균 및 나노기술을 이용한 뿌리혹선충 방제 기술개발 ... 95
• 제7절 천연물을 이용한 선충방제용 compost 개발 ... 103
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 110
• 1절 목표대비 대외 달성도 ... 110
• 2절 정량적 성과 ... 111
• 제5장 연구개발결과의 활용계획 ... 118
• □ 특허기술 이전 활용 ... 118
• □ 기생성곰팡이 Paraconiothyrium minitans S134 균주의 실용화 추진 ... 118
• □ 개발기술의 영농교육 및 현장 기술지원 추진 ... 118
• □ 학술적 성과 제고 및 기초기반 기술 강화 ... 118
• □ 개발기술의 홍보 강화 ... 118
• □ 논문발표 및 게재 ... 118
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 119
• 제7장 기타 중요 변동사항 ... 120
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 121
• 제9장 참고문헌 ... 122
• <참고> 주요 연구성과 요약 ... 132
• 주요 결과 요약서 ... 136
• □ 과제명 : 유용균 및 나노 캡슐 이용 식물병·선충 방제 연구(PJ006741) ... 136