초록 ▼

□ 과제명 : 양돈 분뇨중 악취저감을 위한 사양기술 개발연구
▶ 연구목적 :
○ 1세부과제의 목적은 장내 악취 저감 후보 미생물을 분리하는 것이며, 이를 통해 장내 유용 미생물제를 이용한 악취 저감제 개발 및 축분의 유해가스 감소효과를 구명 하여 악취저감 미생물제 생산시스템을 구축하는 것이다.
○ 2세부과제 목적은 국내외 악취 제거제의 동향과 효능평가 및 Phytogenics (herbs, spices, essential oils)물질을 이용하여 정확한 악취 저감능 DB를 구축 하는 것이며, 더 나아가 복합 천연물질

□ 과제명 : 양돈 분뇨중 악취저감을 위한 사양기술 개발연구
▶ 연구목적 :
○ 1세부과제의 목적은 장내 악취 저감 후보 미생물을 분리하는 것이며, 이를 통해 장내 유용 미생물제를 이용한 악취 저감제 개발 및 축분의 유해가스 감소효과를 구명 하여 악취저감 미생물제 생산시스템을 구축하는 것이다.
○ 2세부과제 목적은 국내외 악취 제거제의 동향과 효능평가 및 Phytogenics (herbs, spices, essential oils)물질을 이용하여 정확한 악취 저감능 DB를 구축 하는 것이며, 더 나아가 복합 천연물질을 이용한 악취저감 premix 제조 및 산업화를 통해 악취 저감제 대량 생산 공정을 확립하는 것이다.
○ 3세부과제의 목적은 장내 악취 발생량 측정법, 단미사료별 악취 발생량 조사 및 분석 방법 표준화를 통하여 단미, 보조사료별 악취발생 DB구축을 확립하는 것이며 더 나아가서 악취저감 사료 배합비를 제시 하는 것이다. 이러한 연구를 통해 본 과제는 악취저감에 대하여 특화된 기술과 DB를 구축함과 동시에 산업최적화를 통하여 국내 축산 업계의 경쟁력과 친환경적 농업에 일조할수 있을 것이다.
▶ 주요연구내용 :
- 장내 악취저감효과분석을 위한 방법개발 및 미생물이용 악취저감제 제조
- Phytogenics 및 효소제를 이용한 악취저감방안 조사
- 유용보조사료/단미사료별 악취발생능조사 및 사료개발
○ 1세부과제명 : 장내유용미생물이용 악취저감재 개발연구
- 악취저감미생물의 분자생물학적 방법을 이용한 동정 및 특성 구명
- 악취저감 미생물 스크린 및생화학적 특성 구명
- 장내용물 및 악취발생물질을 이용한 선택배지 제조
- 단일 및 복합미생물을 이용한 악취저감능 분석
- 천연물질과 혼합시 악취저감능 분석결과 및 활용방안 검토
- 악취발생감소를 위한 미생물제의 최적 배양조건 확립
- 미생물제 첨가에 따른 유해가스 감소효과 현장시험 및 생산시스템 방안 마련
○ 2세부과제명 : 천연물질을 이용한 악취저감prem ix 개발 연구
- 국내의 축사 환경개선 기능 첨가제의 data화
- 축사 환경개선 기능 첨가제의 동향 분석
- 이들 제제의 돈분뇨 내 악취 저감능 평가방법 확립
- 국내산 및 국외산 phytogenics 원료 및 기타 가능성 원료의 확보
- Phytogenics 샘플의 돈분뇨 내 악취 저감능 평가방법 확립
- Phytogenics 원료의 돈분뇨 내 악취 저감능 평가 자료 확보로 사용 가능성 원료 선별 및 확보
- 점토공물질의 악취저감 효능 자료 확보
- 악취저감 효율 증대를 위한 복합 혼합제로서 가능성 검증
- in vitro 돈분발효 악취저감 효능 우수성 확인
- 자돈 사양 시험을 통한 premix의 기호성과 소화 생리에 이상에 문제없음을 확인
- 자돈의 성장 촉진 및 양돈 분뇨의 악취저감 우수하여 산업화 가능성 확인
○ 3세부과제명 : 단미사료별 악취발생량 조사 및 기준설정 활용연구
- 악취측정법 개발 및 분석방법 표준화
- 단미사료를 대상으로 주요 악취물질 분석
- 생균제, 효소제 등 보조사료의 악취저감능 평가
- 사양시험을 통한 악취저감사료 효능 평가

Abstract ▼

Ⅳ. Results of research development
1. Beneficial intestinal microorganisms of deodorant development
Identification result of beneficial intestinal microorganisms using selective medium, Enterococcus faecium CWBI-B1430, Enterococcus faecium DSM 20477, Enterococcus faecalis ATCC 19433, Lactobac

Ⅳ. Results of research development
1. Beneficial intestinal microorganisms of deodorant development
Identification result of beneficial intestinal microorganisms using selective medium, Enterococcus faecium CWBI-B1430, Enterococcus faecium DSM 20477, Enterococcus faecalis ATCC 19433, Lactobacillus acidophilus C1, Lactobacillus plantrum C2, Bacillus subtilis strain ZHA10, Bacillus subtilis strain XJAS-ZB-15, Acetobacter malorum LMG 1746, Enterococcus faecium NRIC 0114 were identified. Outstanding ability 4 selected strains Enterococcus faecium (M1), Enterococcus faecalis (M2), Acetobactertro picalis (M3), Bacillussubtilisssp. Subtilis (M4) were tested by in vitro. Total gas production was increased with the advancement of the fermentation period (p< 0.05). pH value dcreasee for 12-24 hours of incubation, except for a of C1 and C2. Gas ammonia concentration from 12 to 24 hours of incubation, quickly decreased except for C1. M1 concentrations were lowest in treatment. liquid ammonia concentration was increased, except for M1, M4. M4 treatments showedthe lowest concentration. M1, M3, M4 H2S of concentration and close to 0 rapidly decreased in the treatment. The total BA decreased from 12 to 24 h in the C1, M1, M3, and M4 (p< 0.05) and lowest concentration was in M1 group. TVFA production (p< 0.05)was lowest in the M1 group followed by M4, M2, C1, M3, and C2 at 24 h. Lactate production was higher to lower (p< 0.05)in M1, C2, M4, M3, M2, and C1, respectively. The nitrite (p< 0.05)and sulfate concentration in the liquid phase were higher at 72 h with M1 and M4. when mixed with essential oil and Enterococcus faecium CWBI-B1430, total gas production was reduced the most, the ammonia was measure lowest when Acetobacter tropicalis with the essential oil mixed, and essential oil mixed with Enterococcus faecium. Reduction of hydrogen sulfide was lower in the when mixed with essential oil with Enterococcus faecium CWBI-B1430. One of the odor reducing bacteria Enterococcus faecium was finally selected and used for spray to confirm the odor reduction at different periods in the confinement pig houses. Odor compounds were comparatively lessened up to 3 days after spraying. In conclusion, according to our overall evaluation, E. faeciumcan be used efficiently as probiotic microbes in pigs with carbohydrate overload in the large intestine, in manure slurry and confinement houses by spraying to reduce toxic odorous compounds.
2. Using natural materials and development of odor reducing premix
A. Part of the trend in domestic and efficacy evaluation of odor remover
The Environment Improving Agents of main ingredients are include microorganisms, enzymes, plant extracts (Essential oil), organic acids, minerals. Microbes agents are 68% and enzyme Complex (17%), minerals (7%), Microbiology - enzymes - Complex Plant Extract (3%), plant extracts (3%), respectively.
B. Ingredients properties and investigation of Phytogenics`s Odor Reduceing Ability
Ammonia content in manure for agricultural products compared, In conclusion, persimmons peel, mandarin peel, seaweed powder, red ginseng meal found good reduction activity in the experiment. among the hubs Ammonia production seems to suppression persimmon, chrysanthemums, chrysanthemums of leaf, pine of leaf, Ginkgo (yellow, green). Quillaia were selected Essential oil of assessment results were superior to cinnamic aldehyde and Origanum compactum.
C. Using phytogenics, binder, and enzyme activity of the odor occurrence ability survey
When Precipitated Calcium Carbonate, Elvan, irregular, bentonite, magnesium oxide, ceramic powders, eksal, eksal H, zeolite and klinofeed containing 0.5% is add, when Odor reduction efficiency consider, zeolite and klinofeed is considered as efficient raw materia And hub + enzyme complex of ammonia content was lower than a single hub.
D. Using natural materials to odor reduction premix for manufacturer and industrial
Herb that Odor reduce effect is outstanding and functional herb ingredients mixture (Glycyrrhiza uralensis Fischer, artemisia capillaris, garlic, dried orange peel , Ginkgo biloba) divided into treatment 10 goats and The feed mixture as 0.1% feed to a swine for 4 weeks. Body weight gain in one day, natural herb treatments showed higher than non-feed, while piglets of diarrhea incidence occurred once a 4 weeks, treatment fed natural herbs was not detected and palatability problem was not observed for experiment. Treatment fed natural herbs was showed lower than non-fed treatment in the stench of feces related substances (phenol, cresol, indole). the ammonia and hydrogen sulfide caused from manure was showed low level in the treatment fed natural herb.
3. Feed ingredients of odor emission standards set and utilization survey
A. Feed stuffs`s Odor emission of in vitro / in vivo assay development
Microbial group to provide be taken from the anus in order to obtain swine minutes directly. Prepared anaerobic salt medium (1atm, 121℃, 15 min) and then cooled at room temperature, and cysteine remove impurities by using a filter and then placed ready. Manure 10g contains quickly in the beaker(250ml), and after put in the anaerobic salt medium 100ml homogenize. Fluorinated solids isolated by gauze and after puting into a bottle (250mL), CO₂ release. cysteine(1ml) was added in the CO2 released manure dilution. Finally, the slurry100g per L is prepared, the sample is analyzed based on the odor compounds.
B. Feed stuffs of odor emission measurements(2009)
Concentration by animal protein and vegetable protein was measured in 329, 196ppm and higher than cereals and brans in 69, 88ppm. Looking at different types of feed grains, as phenol concentration 19.0 ~ 57.2ppm, brans 7.8 ~ 47.6ppm, Vegetable Proteins 24.0 ~ 246.9ppm, vegetable protein, whole grains and brans Flow 15.4 ~ 86.9ppm. The protein concentration range was shorter than the concentration range of vegetable proteins. Histamine concentration of control was highest with 459.5ppm. Next, concentration of probiotic was higher in 320.7ppm. Total amine was also highest in 887ppm, probiotics have been measured in 863ppm. VFA concentration of the secondary feed in the protein diet was measured to the highest acetic acid and propionic acid in probiotic treatments were the highest.
C. Field Testing and Evaluation of Odor Feed Specification(2010)
10 tapioca of average daily gain was highest in four weeks. the control group was higher than tapioca 20, also feed intake of changes is the most high in tapioca 10. The control was more than 20 tapioca. Changes of feed demand rate was highest in the control group, was followed by tapioca 20. The tapioca 20 was measured lowest by odor analysis.

목차 Contents

• 제출문 ... 1
• 요약문 ... 2
• SUMMARY ... 10
• CONTENTS ... 18
• 목차 ... 19
• 제1장 서론 ... 20
• 제1절 연구개발의 중요성 ... 20
• 제2절 연구개발의 목표 ... 21
• 제2장 국내외 기술개발 현황 ... 24
• 제1절 국내 현황 ... 24
• 제2절 국외 현황 ... 25
• 제3장 연구개발수행 내용 및 결과 ... 26
• 제1절 장내 유용미생물 이용 악취 저감제 개발연구 ... 26
• 제2절 천연물질을 이용한 악취저감 premix 개발연구 ... 61
• 제3절 단미사료별 악취발생량 조사 및 기준설정활용 연구 ... 89
• 제4장 연구개발목표 달성도 및 대외기여도 ... 108
• 제1절 목표대비 대외달성도 ... 108
• 제2절 정량적 성과 (단위: 수량 / 편, 건) ... 110
• 제5장 연구개발결과의 활용계획 ... 114
• 제1절 장내 유용미생물이용 악취저감제 개발연구 ... 114
• 제2절 천연물질을 이용한 악취저감 premix 개발연구 ... 114
• 제3절 단미사료별 악취발생량 조사 및 기준설정 연구 ... 115
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 116
• 제7장 참고문헌 ... 119
• 주요 결과 요약서 ... 122