보고서 정보
주관연구기관 |
국립농업과학원 |
연구책임자 |
권순우
|
참여연구자 |
김수진
,
김이슬
,
원항연
,
박진우
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2012-02 |
과제시작연도 |
2011 |
주관부처 |
농촌진흥청 |
사업 관리 기관 |
농촌진흥청 Rural Development Administration |
등록번호 |
TRKO201400000630 |
과제고유번호 |
1395022087 |
DB 구축일자 |
2014-05-07
|
초록
▼
□ 과제명 : 세균 유전자원 분류 및 관리기술 개발
▶ 연구목적 :
○ 농업 및 식품 유래 세균 다양성 확보 및 활용기반 구축
○ 농업 및 식품 유래 세균에 대한 분류연구 및 신규 자원의 확보
▶ 주요연구내용 :
○ 1세부과제명 : 세균 자원 종합관리 연구
- 세균자원 다양성 확보 및 KACC 등록 : 2669균주
- 특허미생물 수탁, 보존 및 관리 : 378균주
- 미생물자원 품질검정 : 총 6,500균주에 대한 염기서열 분석 및 품질검정
- 세균유전자원 분양 : 연구용 소재로 대학
□ 과제명 : 세균 유전자원 분류 및 관리기술 개발
▶ 연구목적 :
○ 농업 및 식품 유래 세균 다양성 확보 및 활용기반 구축
○ 농업 및 식품 유래 세균에 대한 분류연구 및 신규 자원의 확보
▶ 주요연구내용 :
○ 1세부과제명 : 세균 자원 종합관리 연구
- 세균자원 다양성 확보 및 KACC 등록 : 2669균주
- 특허미생물 수탁, 보존 및 관리 : 378균주
- 미생물자원 품질검정 : 총 6,500균주에 대한 염기서열 분석 및 품질검정
- 세균유전자원 분양 : 연구용 소재로 대학, 국공립연구소 등 총 4,669균주 분양
- 농업미생물유전자원 관리기관 운영 : 9개소
○ 2세부과제명 : 농업환경 유래 세균자원 관리 및 분류동정연구
- 다양한 농업환경으로부터 세균자원 분리 : 토양, 공기, 물 폐면 등
- 세균자원에 대한 기질 분해능 검정 : cellulose 등
- 세균자원에 대한 분류학적 연구 : 총 2속 및 27종의 시규 분류군 발굴 및 보고
○ 3세부과제명 : 식품 유래 세균자원 관리 및 분류동정연구
- 식품유래 세균 확보 : 수집, 제조한 발효식품에서 세균분리 및 보존(1,374균주)
- 식품 유래 세균자원에 대한 분류학적 특성 평가 : 16S rRNA 염기서열 분석
- 식품 유래 세균자원의 활용도 제고 : 200균주
○ 4세부과제명 : 방선균 관리 및 분류동정연구
- 방선균 수집 및 보존 : 150균주
- 방선균의 분류학적 특성 평가 : 16S rRNA gene sequencing 및 계통분석 등
- 방선균의 분류학적 특성 평가 : 세포지방산 등
- 방선균의 기능적 특성 평가 : 효소활성 및 항균 활성 조사
○ 5세부과제명 : 특수환경 서식 미생물의 농업·환경적 기능탐색 및 활용
- 특수환경 서식지 미생물 유전자원 확보 : 미생물 유전자원 확보
- 친환경 농자재 활용 가능 미생물 기능 검정 : 활용가능 우수기능 탐색 여부
○ 1협동과제명 : 일본미생물자원센터 (NBRC) 보유 미생물자원 및 분류기법 도입
- NBRC 미생물유전자원 도입 : 666 균주
- 방선균 화학적특성(Peptidoglycan) 분석 : 국내 농업유래 방선균의 12균주
Abstract
▼
A total of 2,669 bacterial strains were newly registered in KACC for last tree years. Until now, A total bacterial strains in KACC have increased up to about 3,500 species and 6,766 strains. These bacterial strains were safely preserved using liquid nitrogen, lyophilization and ultra-low temperature
A total of 2,669 bacterial strains were newly registered in KACC for last tree years. Until now, A total bacterial strains in KACC have increased up to about 3,500 species and 6,766 strains. These bacterial strains were safely preserved using liquid nitrogen, lyophilization and ultra-low temperature preservation methods. For the quality control, KACC sequenced partial 16S rRNA gene of nearly all bacterial strains. Among the strains held in KACC, 96% (6,530 strains) were subjected to 16S rRNA gene sequencing. 91% (6,170 strains) were found to be authentic while 5%(360 strains) were mis-identified or did not show the growth. KACC has distributed its bacterial strains to universities, public research institutes and private companies. For last tree years, a total of 4,669 strains were distributed for the research purposes. KACC also provided the domestic patent material deposit service. The patent biological materials which can be deposited to KACC were bacteria, fungi, mushrooms, viruses, genes and plant seeds. A total of 378 materials were safely preserved in KACC. As a main microbiological bank for agricultural genetic resources, KACC built the network with 12 sub-banks. The sub-banks collect ed microorganisms from various environments and the well-characterized ones were deposited to KACC. All the bacterial strains which can be open to the pubic were data-based on RDA genebank(http://www.genebank.go.kr/) and used for the microbial researches.
The bacterial isolates from soil, air and water samples were subjected to taxonomic study. Polyphasic taxonomic approach including the morphological, physiological and molecular characterizations were conducted. A total of 29 new taxa were reported with 2 new genus and 27 new species. For the putative new species classified into the phylum proteobacteria, we conducted degradation ability for starch, CMC, Tween80 and casein. The strains degrading more than one substrates were about 200 strains, and several strains revealed degradation ability for over three substrates.
Fifty six Korean traditional fermented food were collected from different regions and manufactures from 2009 to 2010. In 2011, Jang was prepared using traditional method with the help of a manufacturer in Gyonggi province. To isolate various bacterial strains, MRS agar, Raka-Ray agar, Rogosa agar, and R2A agar were used and a total of 1,374 species were isolated and preserved. Data obtained by cultivation-based methods showed that Bacillus species had the highest percentage (49.2 %) and the percentage of lactic acid bacteria were 45.9 %. Also, the bacterial diversity and community structure in samples were examined using barcoded pyrosequencing of the 16S rRNA gene. The result shwoed that the bacterial communities in Korean fermented soybean food were very diverse and a complex microbial consortium containing various microorganisms.
Taxonomic and functional characterization were carried out for actinomycetes strains isolated from various habitats such as soil and atmosphere. Predominant actinomycets genera were Arthrobacter , M icrobacterium, Nocardioides and P hycicoccus, accounting for 52% of a total. According to taxonomic characterization, 3 novel species, Tsukamurella soli, Terrabacter aeriphilus and M icrobacterium suwonense were described. In functional characterization, 112 strains (58.6%) and 73 strains (38.2%) of 192 actinomycetes strains showed antagonistic effects against Botrytis cinerea and Sclerotinia sclerotiorum, respectively.
A total of 38 strains, which had cellulase activities, have been isolated from soil and rotting tree, and among them, 5 strain with strong cellulase activities were selected. 16S rRNA gene sequences analysis showed that the strains were closely related to Micromonospora matsumotoense, Kitasatospora gansuensis and Strptomyces flavovirens, and it was revealed that they can promot the dehiscence of ginseng seeds.
Bacterial strains were also collected from special habitats such as tidal mudflat, salted seafood and forest soils including Halla mountain. Totally, 2,065 bacterial strains were isolated and tested for their physiological characteristics of antagonistic and proteolytic activities, and auxin production. Among the isolates, 368 strains were selected as antifungal strains showing antagonistic activity against at least one of 8 plant fungal pathogens. In addition 284 strains for proteolytic activity and 27 strains for auxin production were characterized for further study. One hundred thirty-two strains were identified by fatty acid phylogenic difference analysis from MIDI shorlock gas chromatography system and 16S rRNA analysis. As a result, 81 strains of Bacillus and 15 strains of P aenibacillus were identified, and they were dominant genus.
Through the cooperative study between KACC and NBRC (Japan), KACC introduced a total of 666 bacterial strains from NBRC. These strains were preserved in proper methods, and open to the public. Most of the strains introduced from NBRC were the type strains and reference starins, and were expected to be widely used for the microbial researches. We also studies taxonomic studies about Actinomycetes strains isolated from Korea. NBRC analyzed the peptidoglycan structures in cell walls and KACC conducted characterization including morphological and physiological and molecular propertes for these strains.
목차 Contents
- 제출문 ... 1
- 요약문 ... 2
- SUMMARY ... 6
- 제1장 서 론 ... 8
- 제2장 국내외 기술개발 현황 ... 10
- 제3장 연구개발수행 내용 및 결과 ... 11
- 제1절 세균자원 종합관리 연구 ... 11
- 제2절 농업환경 유래 세균자원 관리 및 분류동정연구 ... 23
- 제3절 식품 유래 세균자원 관리 및 분류동정 연구 ... 46
- 제3절 방선균 관리 및 분류동정연구 ... 65
- 제5절 특수환경 서식 미생물의 농업, 환경적 기능탐색 및 활용 ... 82
- 제6절 일본미생물자원센터 (NBRC) 보유 미생물자원 및 분류기법 도입 ... 91
- 제4장 연구개발목표 달성도 및 대외기여도 ... 118
- 제1절 목표대비 대외달성도 ... 118
- 제2절 정량적 성과 ... 119
- 제5장 연구개발결과의 활용계획 ... 120
- 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 120
- 제7장 기타 중요 변동사항 ... 120
- 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 120
- 제9장 참고문헌 ... 120
- 주요 결과 요약서 ... 130
※ AI-Helper는 부적절한 답변을 할 수 있습니다.