초록 ▼

○ 연구결과
1. 오리나무 용매 분획물 중 활성이 강한 dichloromethane 및 ethylacetate 용매 분획물로부터 총 11개의 화합물을 단리하였고, 이화학 기기분석을 통해 화학구조를 규명하였다.
2. 오리나무 수피 열수 추출획분(1g)을 증류수에 녹여 DIW로 평형화시킨 DEAE-Sepharose FF column(Cl-form, 4.0×30 cm)에 흡착시킨 후 DIW, 0.1, 0.2, 0.3, 0.4, 0.5, 1.0, 및 2.0 M NaCl 용액으로 차례로 용출시켜 각 획분은 투석, 농축 및 동결 건

○ 연구결과
1. 오리나무 용매 분획물 중 활성이 강한 dichloromethane 및 ethylacetate 용매 분획물로부터 총 11개의 화합물을 단리하였고, 이화학 기기분석을 통해 화학구조를 규명하였다.
2. 오리나무 수피 열수 추출획분(1g)을 증류수에 녹여 DIW로 평형화시킨 DEAE-Sepharose FF column(Cl-form, 4.0×30 cm)에 흡착시킨 후 DIW, 0.1, 0.2, 0.3, 0.4, 0.5, 1.0, 및 2.0 M NaCl 용액으로 차례로 용출시켜 각 획분은 투석, 농축 및 동결 건조하여 획분을 얻었다. 이러한 획분들 중 가장 활성이 높은 획분에 대하여 0.2 M NaCl용액으로 평형화된 Sepharose CL-6B column(2.5×94 ㎝)에서 gel filtration chromatography를 행하여 획분을 얻었다.
3. 오리나무 수피의 당 분석결과 오리나무 열수 추출물중 메탄올 불용성 분획은 arabinose와 galactose로 구성된 다당체, arabinose, mannose, galactose로 구성된 다당체, rhamnose, arabinose, xylose, mannose로 구성된 다당체, arabinose, xylose, mannose로 구성된 다당체, arabinose, xylose, mannose로 구성된 다당체로 밝혀졌다.
4. In vitro에서 H. pylori에 대한 항균력을 갖는 오리나무 분획은 OMD가 가장 좋은 활성을 나타내었으며, OAD나 OMD에서 공통적으로 존재하는 물질에 의할 것으로 사료되었다.
HCl-EtOH로 유도한 rat 급성 위 손상 모델에서 다양한 오리나무 추출물들 즉 열수, methanol 및 acetone 추출물들이 위염이나 위궤양 치료제로 사용되는 cimetidine이나 omeprazole 보다는 약하나 급성 위 손상으로부터 유의적인 보호 작용이 나타남을 확인하였다.

Abstract ▼

In South Korea four woody plant species which are belong to Bignoniaceae, grow in open space. It consists of two genera(Catalpa and Campsis), each genus has two species. Genus Catalpa(Catalpa ovata and C. bignonioides) is tall straight tree,are planted in the garden or along the street but Campsis's

In South Korea four woody plant species which are belong to Bignoniaceae, grow in open space. It consists of two genera(Catalpa and Campsis), each genus has two species. Genus Catalpa(Catalpa ovata and C. bignonioides) is tall straight tree,are planted in the garden or along the street but Campsis's species(Campsis grandflora and C. radicans ) are creepers. and grow on the fence or on the trees, All of them are from foreign countries( Catalpa ovata and Campsis radicans are from China, and others from North America.
For the industrialization of functional natural products from Bignoniaceae trees, a series of several projects were conducted such as an evaluation of pharmaceutical value and physiological characteristics of extracts from different parts of each trees, establishment of propagation method by cutting, axillary bud culture, somatic embryogenesis and cell culture.
To screen the physiological functions on neurodegenerative disorders, the effects on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells were investigated using the various bioactive components from Catalpa ovata and Campsis grandiflora. The bioactive components from the stem barks and fruits Catalpa ovata and Campsis grandiflora were isolated routinely using the active-guided fractionation methods and determined by the various instrumental analyses. Finally, 20 compounds from Catalpa ovata and 13 compounds of Campsis grandiflora were isolated and identified.
Among the various bioactive components, catalpalactone at 20 μM decreased dopamine content to 65% of control levels by reducing tyrosine hydroxylase (TH) and aromatic L-amino acid decarboxylase (AADC) activities in PC12 cells. Catalpalactone also inhibited L-DOPA-induced increases in dopamine content and enhanced L-DOPA-induced cytotoxicity in PC12 cells.
In contrast, catalponol (CO-F7) at 1-5 μM and 4,9-dihydroxy-α-lapachone (H8-2-2) at 1-3 μM increased intracellular dopamine content to 110-145% of control levels by inducing TH activity in PC12 cells. Intracellular cyclic AMP levels and phosphorylation of TH protein were also enhanced by CO-F7 and H8-2-2. In addition, CO-F7 and H8-2-2 enhanced L-DOPA-induced dopamine increases and showed a protective activity against L-DOPA-induced cytotoxicity, which were proved by flow cytometry histograms, in PC12 cells. These results suggest that CO-F7 and H8-2-2 have a possibility to apply for the dopamine-related neurodegerative disorders.
The establishment of biomass production was coducted in the present study along the cutting period, plant growth hormones, characteristics cutting, and container media in trees belonging to Bignoniaceae.
Concerning the characteristics of rooting in cuttings, the rooting showed different vigour in the order of dormant wood cuttings(April) > semihard wood cuttings(June) > green wood cuttings(May), and almost no rooting was in green wood cuttings. The semihard wood cuttings showed low rooting rate, 20% or less, and treatment of Rootone and IBA showed relatively higher rooting rates compared to control. The rooting rate more vigorous in Catalpa ovata than Catalpa bignonioides.
The effects of plant hormones on rooting were in the order of Rootone > IBA > NAA > ABA in dormant wood cuttings. The effects was stronger in Catalpa ovata than in Catalpa bignonioides.
The 1:1:1(peatmoss, pearlite, vermiculite) container medium showed the highest rooting rate by plant hormone treatment in dormant wood cuttings, and Catalpa ovata showed high rooting rate in IBA 1:1:2(peatmoss, pearlite, vermiculite) and 1:2:1(peatmoss, pearlite, vermiculite) on rooting media and Catalpa bignonioides in Rootone plus 1:1:1:1(peatmoss, pearlite, vermiculite, bark) and 2:1:1 (peatmoss, pearlite, vermiculite) of rooting media. The biomass productions of leaf and stem were irregular according to the characteristics of the cuttings such a thickness and age however the dry weight of root increased with rooting rate in a high correlation between the rooting rate and biomass production of the root.
For further research and conservation of germplasm, a clonebank was established with these woody species.
For propagation of these species micropropagation technique was adopted. Three species(two Catalpas and Campsis chinensis) were successful, other one is under progress. With juvenile materials, BAP(6-Benzil aminopurine) was effective as additive to WPM solid media for production of multiple shoots and microshoots was possible to be rooted on solid GD media with IBA. Plantlets of all three species survived acclimatization.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 요 약 문 ... 5
• SUMMARY ... 7
• CONTENTS ... 9
• 목 차 ... 10
• 제 1 장 연구개발과제의 개요 ... 11
• 제 1 절 연구개발의 목적 ... 11
• 제 2 절 연구개발의 필요성 ... 14
• 제 2 장 국내외 기술개발 현황 ... 17
• 제 1 절 우리 나라의 기술개발 현황 및 개발 수준 ... 17
• 제 3 장 연구개발수행 내용 및 결과 ... 19
• 제 1 절 생물공학에 의한 유용물질생산방법 개발 ... 19
• 제 2 절 능소화과 자원의 약리활성 성분 탐색 및 약효연구 ... 58
• 제 3 절 자원수집 및 유􀂂무성증식법 개발 ... 84
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 108
• 제 5 장 연구개발결과의 활용계획 ... 109
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 109
• 제 7 장 참고문헌 ... 110
• 끝페이지 ... 116