초록

○ 연구결과
① Mycorrhiza와 PGPR 균의 기능성 강화 및 지속성 유지를 위한 배지의 적정 환경 조성
② Mycorrhiza와 PGPR 균의 육묘, 포장에서 생장촉진, 병충해에 대한 기능성 검증
③ Mycorrhiza와 PGPR 균의 광범위한 적용성 검토를 통한 산업화 기술 확립
④ Mycorrhiza와 PGPR 균의 대량 생산 제조법 확립
⑤관련기술: Mycorrhiza와 PGPR 균의 대량생산의 특허 및 제품화

Abstract ▼

The spores of indigenous arbuscular mycorrhizal fungi(AMF) were collected from rhizosphere of Mt. Mu-Deung(Kwang-Ju), Dae-Won-Sa(Bo-Sung) and Man-Yon-Sa(Hwa-soon). Spore density in soil was 200(Kwang-Ju), 42(Bo-Sung) and 83(Hwa-soon)spores/g dried soil.
200 spores/g soil isolated from Mt. Mu-deun

The spores of indigenous arbuscular mycorrhizal fungi(AMF) were collected from rhizosphere of Mt. Mu-Deung(Kwang-Ju), Dae-Won-Sa(Bo-Sung) and Man-Yon-Sa(Hwa-soon). Spore density in soil was 200(Kwang-Ju), 42(Bo-Sung) and 83(Hwa-soon)spores/g dried soil.
200 spores/g soil isolated from Mt. Mu-deung were planted in the soil of sudangrass to multiply AMF spores. The density of AMF spores was increased two times. Also root colonization was certified by the root staining process that endomycorrhizal hyphae, arbuscules and vesicles were observed.
PRPR(plant Growth promoting Rhizobacterimum ) strains were selected and propagated after being collected from the rhizosphere soil of tea plantation in Junnam province. PGPR-ability-strains such as Tea-1(Burkholderia sp.), Tea-2(Staphylococcus sciuri), Tea-3, Tea-4, Tea-5(bacillus subtilis), Tea-6 were separated.
The optimal methods for rooting cuttings was in 10 cm scion long, IBA 40ppm root-promoting hormone concentration and 18hr hormone treatment. The rooting rate was 83.3%.
Colonization rate during AMF multiplication in sudangrass was 95.45% but the colonization rate was 31.10% when to tea cutting roots the spores inoculated in this processes.
Chitinase Producing bacteria density , chitinase activity and β-1,3-Glucanase activity revealed the highest on Entire Mixed Strains(EMS) treatment group and Semi Mixed strains(SMS) treatment group was higher than single strain treatment group control.
Rooting effects on tea cuttings inoculated with AMF were that number of adventious roots was 6 on control but 21 on EMS treatment group. The biomass of adventious roots was the most at the EMS treatment group.
The rooting effect on tea cuttings inoculated with PGPR were that number of adventious roots was 6 on control but 21 on EMS treatment group. The biomass of adventious roots was as 188.6g the most at the EMS treatment group.
The root cuttings inoculated with PGPM revealed significantly more number of leaves on EMS treatment group respectively. 4.53(July) and 5.70(August) while on single strain was 2.67 in September. Shoot length was the most on My treatment, root collar diameter on My + EMS treatment group, shoot dry weight on My + EMS treatment group and roots dry weight in EMS group. Chlorophyll content was significantly 67.10 mg/100㎠ on My treatment.
When Juvenile seedlings were inoculated by PGPR, these showed growth stimulation effect by increasing of EMS concentration.
Peatmoss +vermiculite treatment group was the best growth on height length. Root collar diameter of forest C layer soil(FCL) was the most compared to the others and root growth was more than shoot growth on FCL treatments. The most suitable mixture soil was Peatmoss+Vermiculite+perlite on leaf area. Seedlings height growth(SHG) was significantly the most effect at 2 week application interval, root collar(RC) diameter was at 2 week application interval, shoot dry matter weight(SDW) was at 2 week application interval, root dry matter weight(RDW) was at 1 week application interval and leaf area(LA) was at 1week application interval. They showed the best growth effects as recorded 14.17cm(SHG), 1.06g(RC), 0.28g(SDW) and 44.27㎠(LA) respectively compared to the others.
The quality improvement effect of tea leaves by inoculation of PGPM was evaluated. In results, Amino acid content was 836.33 mg/100g on control ; Tannin content was 12.06% on control compared to 10.54% on My ; Caffeine content was 2.01% on control ; Catechine content was 12.74%. It showed the highest value each. Especially Vitamin content was high significantly 334.21mg/100g on PGPR +My compared to 230.04mg/100g on control.
As shading rate was increased, shoot dry matter weight was decreased. while, Root dry matter weight was increased. Also, Chlorophyll content and total amino acid showed being increased. Theanine that have used as one of the indicator determining tea taste was increased abruptly at the point of 55% (shading rate). Vitamin content was the highest at 55% and showed steep reduction at 75%. Tannine content at 55% and caffeine content at 95% was 11.47% and 2%, high concentration each.
Practical use of PGPM on tea plantation was evaluated. Treatment by PGPM seems to increase plant growth, improving quality of tea leaves.
We tried ascertain whether AMF and PGPM is efficacious in alleviating the deleterious effect of anthracnose in Tea plant. Furthermore, we undertook to explain whether changed PR-protein and antioxidant enzyme activities could alleviate protection affection. Major changes in PR-protein(20%) and antioxidant enzyme(60%) activities in PGPM and AMF+PGPM made us confirm the efficacy of AMF and PGPM in control of anthracnose in Tea plant.
The invented technology on the production of PGPM in large scale by special substrate may be readily popular to the farmers because of it's low cost and easy handling. We have tried to cultivate mass production of AM inocula. In present AMF propagules from tea root directly it was dual-cultured with hairy root of Bupleurum falcatum.

목차 Contents

• 제 출 문 ... 1
• 요 약 문 ... 2
• SUMMARY ... 6
• CONTENTS ... 9
• 목 차 ... 10
• 제1장 연구개발과제의 개요 ... 11
• 제1절 연구개발의 필요성 및 범위 ... 11
• 제2절 연구개발의 목적 ... 15
• 제2장 국내외 기술개발 현황 ... 18
• 제1절 국내 ... 18
• 제2절 국외 ... 19
• 제3절 현 기술 상태의 취약성 ... 20
• 4절 앞으로의 전망 ... 21
• 제3장 연구개발 수행내용 및 결과 ... 22
• 제1절 AMF 와 PGPR균 분리 및 증식 ... 22
• 제2절 AMF 및 PGPR 접종효과 ... 26
• 제3절 AMF 및 PGPR 감염확인 ... 32
• 제4절 삽목의 PGPM(AM + PGPR) 효과 ... 40
• 제5절 차나무 유묘의 PGPM 효과 ... 46
• 제6절 PGPM (AMF+PGPR) 접종에 의한 차엽 품질분석 ... 63
• 제7절 비음처리에 의한 효과 ... 70
• 제8절 차재배지에서의 실제적용 ... 78
• 제9절 PR Protein 분석 ... 86
• 제10절 Antioxidantive Enzyme 분석 ... 91
• 제11절 병제어 조사 ... 98
• 제12절 AMF 및 PGPR 대량생산 ... 106
• 제4장 목표달성도 및 관련분야에의 기여도 ... 113
• 제5장 연구개발결과의 활용계획 ... 116
• 제6장 참고문헌 ... 117
• 끝페이지 ... 119