초록 ▼

○ 연구결과
1. 기능성균주(장수버섯, 잎새버섯, 비늘버섯, 차가버섯) 수집 및 유연관계 분석
- 장수버섯 19, 잎새버섯 35, 비늘버섯 41, 차가버섯 12균주 수집
- URP primer에 의한 핵산지문법, ITS sequencing 수행 균주간 유연관계 분석
- 대표종에 대한 ITS sequence를 GenBank에 등록
2. 기능성버섯 균주의 인공재배법 개발
- PDA, MCM, YM, MEA배지에서 버섯 종류별 균주별 최적배지 선발 및 배양적 특성 조사
- 톱밥수종별 균사생장량 조사와

○ 연구결과
1. 기능성균주(장수버섯, 잎새버섯, 비늘버섯, 차가버섯) 수집 및 유연관계 분석
- 장수버섯 19, 잎새버섯 35, 비늘버섯 41, 차가버섯 12균주 수집
- URP primer에 의한 핵산지문법, ITS sequencing 수행 균주간 유연관계 분석
- 대표종에 대한 ITS sequence를 GenBank에 등록
2. 기능성버섯 균주의 인공재배법 개발
- PDA, MCM, YM, MEA배지에서 버섯 종류별 균주별 최적배지 선발 및 배양적 특성 조사
- 톱밥수종별 균사생장량 조사와 병재배 및 포트재배를 통한 자실체 형성 조건을 조사하여 재배법을 개발
- 버섯종류별 병배배법, 포트재배법, 상자재배법, 원목재배법을 개발하였음.
3. 기능성버섯 품종육성
- 장수버섯 68개 단핵균주, 비늘버섯 184개 단핵균주 육성, 잎새버섯 28개 단핵균주 육성
- 버섯 종류별 단핵균주를 분리하여 단핵균주간 교배 및 Di-mono 교배를 통한 이 핵균주를 육성하였음.
- 기능성 버섯으로 비늘버섯, 잎새버섯, 장수버섯 각 1균주를 선발함
- 농가 확대재배시험 중임
- 그 외 맛버섯과 띠비늘버섯의 재배가능성이 검토됨
- 교배주 및 육성품종의 DNA finger printing으로 pattern 분석
4. 기능성관련 표지형질개발
- 항산화 활성 검정법 비교검토
- 비늘버섯의 생육단계별 SOD 활성비교
- 비늘버섯 자실체에서 cDNA library 작성
- 항산화와 관련된 SOD 유전자 분리(Mn-SOD, Fe-SOD gene을 cloning)

Abstract ▼

Ⅳ. Major Results and the Suggestion for application
Item1: Development of funtional superior strains and artificial cultivation methods
1. Strains collection and analysis of phylogenic relationships
1) Nineteen strains of Fomitella fraxinea, 35 strains of Grifola frondosa, 41 strains of Pho

Ⅳ. Major Results and the Suggestion for application
Item1: Development of funtional superior strains and artificial cultivation methods
1. Strains collection and analysis of phylogenic relationships
1) Nineteen strains of Fomitella fraxinea, 35 strains of Grifola frondosa, 41 strains of Pholiota spp., 12 strains of Inonotus obliquus were collectedand examined.
2) To classify species, the internal transcribed spacer regions (ITS) of the ribosomal DNA(rDNA) repeats were amplified using polymerase chain reaction (PCR) and then sequenced. According to the analysis of ITS sequences, Pholiota spp. were classified into five clusters. Their spacer regions were 644∼700 nucleotides in length. The reciprocal homologies of each ITS region among these strains were ranged from 49.6∼99.9%. Fomitella fraxinea were amplified 592 and 595 nucleotides in length (GenBank AY251308). Grifola frondosa were 616 and 626 nucleotides in length (GenBank AY251307). The reciprocal homologies of each ITS region among these strains were in the range of 94 to 99.7%. Inonotus spp. 9 strains were compared and were classified into five clusters. The spacer regions of them were 687∼758 nucleotides in length. The reciprocal homologies of each ITS region among these strains were in the range of 71.2∼99.7%.
3) URP primers of 20-mer derived from repetitive sequence of rice were used to assess genetic relationships. According to the analysis URP-RAPD, 17 strains of Fomitella fraxinea were classified into several clusters. Genetic similarities based on RAPD was 77 to 100%. Grifola frondosa were classified into several clusters. Genetic similarities based on RAPD were 77 to 100%. Five URP primers produced strain-specific PCR polymorphic bands showed that 8 Inonotus strains are genetically clustered into four clusters by UPGMA cluster analysis. The similarity coefficient among Inonotus strains were in the range of 45∼100%. Our results from the analysis of ITS sequences and RAPD suggest that there are more than two species including Inonotus hispidus and I. obliquus in our strains. These polymorphic DNA profiles could be useful not only to discriminate a new developed dikaryons from parental monokaryons but also to identify collected wild strains.
2. Establishment of artificial cultivation methods
1) Cultural characteristics were investigated into collected strains. They were tested on the four different media(PDA, MCM, YM, MEA) and sawdust(Alder, Oak, Pine, Popular). There was a little variation according to the media and sawdust. Most strains showed white colonies, but some strains were brown. Mycelial growth length differed according to the strains. Pholiota species seemed to be better on PDA and popular sawdust although Grifola frondosa were better on MEA.
2) They were induced for fruitbodies production in the 850 ml bottle or 1000 ml plastic film bag filled with different sawdust, on short log and/or sometimes in the plastic box size 40 x 40 cm² with cotton waste according to mushroom species. Their fruitbody productivity and morphological characteristics were measured.
3. Development of new functional mushroom strains
1) Their own spores were collected and germinated for monokaryotic strains. Dikaryons were produced by mono-mono and di-mon mating. The sexuality of tested mushrooms were tetrapolarity. Several hundreds dikaryotic strains were developed by mono-mono crossing within and between parental monokaryons populations. They were cultivated in the bottle and showed a big variation in their fruitbody yield. Some of them showed higher productivity and better shapes than parental strains.
2) Finally, one mated strain in each mushroom was selected through several cultivation trials. For the confirmation of their characteristics, they are cultivated in the farm. After field test, they will be referred to the responsible inquiry commission in the end of the year.
3) Also, they were confirmed as new developed dikaryons by DNA fingerprinting.
4. Isolation of marker gene related to functional characters
1) Antioxidant enzyme, superoxide dismutase (SOD) were investigated in order to select medicinal mushroom containing high SOD activity in Pholiota spp.
2) The SOD activity in mycelia extracts of collected strains by using NBT method ranged from 11.5% to 37.9%. The selected strains, above 30% of SOD activity, were ASI 24009, 24013, 24015, 24017, 24018 and 24028. All of them were classified a same group (group A) according to the result of ITS sequence data. Genetic similarities of A group ranged 96∼99.1%. All strains tested showed only MnSOD activities, as no band disappeared when the gel were incubated in the presence of cyanide and hydrogen peroxide.
3) MnSOD gene was cloned and sequenced through cDNA library from fruitbody.
5. Suggestion for application
This study was carried out to develop new functional mushrooms. Each developed strain will be referred to the responsible inquiry commission for cultivar registration in the end of the year after field test.
Item2: Development of New Antithrombotic Substance from Mushroom and Production of Functional Food
Among 52 sample mushrooms, the ethanol extracts of Inontue obliquus ASI 74006 mycelia had potential plate inhibitory activity of 81.2%. After the purification of the plate aggregation inhibitor, an active fraction with 91.6% of inhibitory activity was obtained. The purified plate aggregation inhibitor was a novel peptide with 314 Da of molecular weight. Antithrombotic mushroom drink was also prepared and characterized. All data obtained from this study will be useful in functional food or drug industry.
Consigned Item1: Development of New Antihypertensive Angiotensin Ⅰ-Converting Enzyme Inhibitor from Mushroom and Production of Functional Food
Sample mushrooms(52 species) contained crude proteins of 7.1~56.5%, crude lipid 0.2~4.4% and carbohydrate of 30.3~86.6%. Among 52 samples, the water extracts of Pholiota adiposa ASI 24012 fruiting body had potential ACE inhibitory activity of 66%. After the purification of the ACE inhibitor, an active fraction with an IC₅₀ of 0.44mg was obtained. The purified ACE inhibitor was a novel pentapeptide with 414 Da of molecular weight. Antihypertensive mushroom drink and wine were also prepared and characterized. All data obtained from this study will be useful in functional food industry.
Consigned Item2: Selection of mushrooms with high antioxidative potentials and isolation and identification of physiologically activate chemicals
1) Evaluation and selection of mushrooms with high antioxidative activity using various methods at in vitro
(1) On average, Pholiota spp.collected strains were the highest antioxidative activity (21.96 %), and lowest it on Inonotus obliquus collected strains (10.88 %) among collected 80 mushroom strains. Also, ASI Agaricus brunnescens only showed an inhibitory effect above 30 %. Antioxidative activity was generally distributed from 10 % to 20 % on 38 mushroom strains including the ASI 24004.
(2) SOD activity was the highest inhibition on ASI Agaricus brunnescens (26.83 %), and also Pholiota spp.collected strains (10.67 %) were showed to the highest inhibitory effect on average.
(3) The ASI 74007 (30.09 %) showed the higher inhibition percentage for POD activity, while POD activity was showed to non-inhibitory effect for 55 strains.
(4) DPPH activity generally represented higher inhibition than SOD and POD methods. The ASI F-J (80.51 %) showed the highest inhibition percentage, and then 5 strains including the ASI 74013 showed inhibitory effect below 10 %. Also, it was widely showed to inhibition of both 30% to 50% (37 strains) and 10% to 30% (32 strains).
(5) TBA value was the highest inhibitory effect on ASI Pleurotus eryngii (67.5 %), and 12 strains including the ASI 24005 represented inhibition of above 50 %. Pholiota spp. collected strains (42.94 %) showed the higher inhibition compared to other collected mushrooms, and then there were showed to lower inhibition on ASI Grifola frondosa collected strains (8.92 %).
(6) Regarding the electron spin resonance (ESR) method, the higher and lower groups had signal intensities of 0.25 and 0.83 times the Mn²⁺ standard on average, respectively.
2) Extraction, isolation and identification of antioxidative substances from a selected mushroom
(1) Pholiota adiposa (24027) was extracted by using several solvents as Hexen, EtOAc, MeOH at room temparature and isolated compounds was identified by analysis of IR, MS, ¹H NMR and ¹³C NMR. According to these results, compounds was identified to 7 compounds. The results were follows; (1) 1-Linoleic-2-olein, (2) stigmasterol, (3) 1,4-glucopyranosyl-1’,4’-glucopyranosyl-1'',4''-glucopyranoside, (4） 2',3'-diphosphoryl-1' -propanoxy-β-D-glucopyranoside, (５)　1-linoleio-3-olein, (６） 1-(N,N,N-trimethyl ethyl amino phosphoryl) 2,3-dilinolein ion, （７）Glycerol phosphate.
3) Comparison of antioxidative activity on identified and purified antioxidative compound from mushrooms in vitro and in vivo
(1) Comparison of antioxidative activities in vitro
① In inhibitory effects on growth of P383 murine leukemia cell (IC₅₀), 1-linleio-2-olein and stigmasterol had higher inhibitory effects on 41㎍/㎖ and 50㎍/㎖.
② In evaluation of scavenging radical (RC₅₀), the results were follows : 1-linleio-2-olein (2.2㎍/㎖) > stigmasterol (17.5㎍/㎖) > 1-(N,N,N-trimethyl ethyl amino phosphoryl) 2,3-dilinolein ion (48㎍/㎖) > Glyceryl triphosphate (49㎍/㎖)
③ With cell viabillity by H₂O₂, the higher stigmasterol's concentration and longer culture time, the greater the inhibitory effect on oxidative stress.
(2) Comparison of antioxidative activities in vivo
① In experiment weight gain decreased significantly in the treatment of ethanol compared with saline solution-treated. But there was not significant on weight gain in the treatment of mushroom extract compared with the treatment of ethanol.
② In the treatment of stigmasterol, GOT and GTP had lower activation on serum. However, γ-GTP, total cholesterol, HDL and LDL on serum, there were not affected by the doses of rice extract and ethanol used in this study.
③ The MDA levels in liver, brain and pancreas tissue were decreased to a certain extents in the treatment of mushroom extract compared with treatment of ethanol.
④ The decreased liver GST activities induced by ethanol treatment were somehow restored by adminstration of stigmasterol.
Cooperative Item1: Investigation and utilization of an immunomodulator on mushroom
1. Results of Study
1) As a result of investigating into the mouse strain for screening the bioactivity with an immunomodulating function and the immune reactivitydepending upon a specimen concentration, it was known that BDF1, the hybrid group had the optimum culturing conditions that the specimen concentration was 25㎍/mg and the culturing time was 72 hours.
2) As a result of investigating into the antibody activity of B Lymphocyteof the hydrothermal extract from 104 kinds of experimental strains for selecting strains to produce an immunomodulator, the bioactivity of crude polysaccharide of 24007 and 74006 was revealed to be very strong.
3) As a result of conducting a toxicity test on MTT cell of 24007 and 74006, it inhibited cell's proliferation concentration-dependently in the colon cancer cell strain (HT1080) and the cervical cancercell strain (HeLa), but it had no effect on the colon cancer cell strain (SW480). In view of this result, it is thought that it acts selectively on specific cells.
4) As a result of screening the apoptosis inducing activity of the cancer cell strain of 24007, it was identified that degradation of DNA took place.
5) In view of the fact that as a result of investigating into the expression amount of mRNA of MMPs through RT-PCR, the expression amount of MMP was small in the 24007 treatment group, it is conjectured that the 24007 extract would inhibit metastasis or infiltration of the cancer cell through protein decomposition.
6) The molecular weight of the 74006 extract using SDS-PAGE was about 30~32KDa.
7) In the antidiabetic and obesity-inhibiting experiment using crude polysaccharide of 74006, in the treatment group, it had an effect to inhibit the weight and the blood glucose by 20% and 40%, respectively, in comparison with the untreated group.
8) Most of crude polysaccharide of mycelium of 24007 and 74006 was composed of glucose, and additional constituents thereof were glucosamine, galactose, mannose and others.
9) As a result of conducting a toxicity test on 74006, it was identified that the LD50value was over 2,000mg/kg so that it had no tocicity.
2. Application of the Results
As a product related to an immunologic functionenhancing anti-cancer drug using polysaccharide derived from mushroom, products using Shiitake mushroom and Trametes versicolor were introduced in Japan in 1980's, and since then, such product market has grown steadily. In Korea, products produced by Han Kook Sin Yak using Phellinus linteus are available on the market as an ethical drug developed in Korea. However, a study on development of an anti-cancer immunotherapeutic agent derived from Inonotus obliqua which is known as a material having an excellent anti-cancer immunoactivity has not been conducted at all. In this situation, the study project sponsored by Ministry of Agriculture and Forestry will be performed for thepurpose of developing and using extensively an anti-cancer immunotherapeutic agent or an anti-cancer immuno-juvantiafor treating various cancers, particularly colon cancer without any side effect, which will be produced by using protein polysaccharide obtained from pure culture of mycelium of Inonotus obliqua, which activates the in vivo immunologic function.
Cooperative Item2: Mycelial Mass Culture of Valuable Functional Mushrooms
1. Results
1) Out of 17 strains(4 strains of Fomitella fraxinea, 4 strains of Pholiota adiposa, 4 strains of Inonotus obliquus and 5 strains of Grifola frondosa) examined five strains, Fomitella fraxinea (ASI 17017), Pholiota adiposa (ASI 24027, 24012), Inonotus obliquus (ASI 74006), Grifola frondosa (ASI 9025), were selected for investigation.
2) Optimal conditions for mycelial productivity
(1) Fomitella fraxinea - Malt extract 1%, Yeast extract 0.6%, Glucose 1%, pH 7, mineral (KH₂PO₄ 1g, MgSO₄·7H₂O 1g, FeSO₄·7H₂O 10mg, MnCl₂·4H₂O 7mg, ZnSO₄·7H₂O 4mg, CuSO₄·5H₂O 1mg /L) and 25℃
(2) Pholiota adiposa - Malt extract 1%, Yeast extract 1%, Glucose 1%, pH 6, mineral (KH₂PO₄ 1g, MgSO₄·7H₂O 1g, FeSO4·7H₂O 10mg, MnCl₂·4H₂O 7mg, ZnSO₄·7H₂O 4mg, CuSO₄·5H₂O 1mg /L) and 25℃
(3) Inonotus obliquus - Malt extract 1%, Yeast extract 2%, Glucose 1%, pH 10, mineral (KH₂PO₄ 1g, MgSO₄·7H₂O 1g, FeSO₄·7H₂O 10mg, MnCl₂·4H₂O 7mg, ZnSO₄·7H₂O 4mg, CuSO₄·5H<sub>2/sub>O 1mg /L) and 25℃
(4) Grifola frondosa - Malt extract 1%, Glucose 1%, pH 7, mineral (KH2PO4 1g, MgSO4·7H2O 1g, FeSO4·7H2O 10mg, MnCl2·4H2O 7mg, ZnSO4·7H2O 4mg, CuSO4·5H2O 1mg /L) and 25℃
3) The conditions for mycelial production in large scale
(1) Optimal inoculum ratio was 8% for Fomitella fraxinea (ASI 17017) and Inonotus obliquus (ASI 74006). In case of Pholiota adiposa (ASI 24012) and Grifola frondosa (ASI 9025) was 10% inoculum showed highest productivity while Pholiota adiposa (ASI 24027) required 5% inculum for maximum productivity.
(2) Agitation speed of 120 rpm was optimal for Pholiota adiposa (ASI 24012) and Fomitella fraxinea (ASI 17017) while Pholiota adiposa (ASI 24027) and Grifola frondosa (ASI 9025) grow well in agitation of 150 rpm. But Inonotus obliquus (ASI 74006) showed good mycelial productivity in higher agitation rate.
(3) Mycelial productivity of Fomitella fraxinea (ASI 17017) was optimal with aeration rate of 2vvm but other strains(ASI 24012, ASI 24027, ASI 74006, ASI 9025) grew best with 1.5vvm aeration.
(4) At least 20 minute at 121℃ of sterilization was required.
(5) Mycelial productivity by various types of fermenter was investigated with Fomitella fraxinea ASI 17017. The highest productivity(13.5g/L) was achieved by Air lift fermenter.
4) Mycelial production in industrial scale
(1) Mycelial productivity was investigated with commercial media materials. Pholiota adiposa (ASI 24027) and Grifola frondosa (ASI 9025) grew well in soybean cake B medium(soybean cake 0.5%, glucose 2%). The mycelial production of Fomitella fraxinea (ASI 17017) and Inonotus obliquus (ASI 74006) was good with soybean cake A medium(soybean cake 0.5%, glucose 2%, yeast extract 0.5%).
(2) All the strains showed higher productivity when minerals were added to commercial media.
(3) The optimal pre-culturing method for Inonotus obliquus (ASI 74006), Pholiota adiposa (ASI 24027) and Grifola frondosa (ASI 9025) was Procedure 2 while Fomitella fraxinea (ASI 17017) was Procedure 1.
(4) Mycelial productivities of chemical media and commercial media were compared. Higher activity, in general, was achieved with commercial media.
(5) The commercial production test by use of mushroom spawn fermenter(working volume : 150L) resulted in 675g of dried mycelium of Fomitella fraxinea ASI 17017 in 7days, 570g of dried mycelium of Pholiota adiposa ASI 24027 in 10days, 390g of dried mycelium of Inonotus obliquus ASI 74006 in 10days and 615g of dried mycelium of Grifola frondosa ASI 9025 in 10days.
2. A Suggestion for application
The data obtained in this study could be applied for commercial production of medicinal mycelia. The 200 liter capacity culture vessel is handy, simple and less risky against the contamination problem compared to large scale tank used in large industries.
Cooperative Item3: Development and Utilization of Functional Mushroom, Fomes Fomentarius and Kuehneromyces mutabilis
The study was carried out for the hypha culture and artificial cultivation technique for the Fomes Fomentarius and Kuehneromyces mutabilis. The appropriate conditions were revealed for the characteristics of culture for mushroom's hyphae such as pH, the concentrate of carbohydrate, the kinds and concentrates of nitrogens, and temperature for culture. he characteristics for artificial culture of the mushroom were revealed.
Then, the artificial culture was performed to get fruit bodies as shown in the text of this article. The functionality of Fomes Fomentarius and Kuehneromyces mutabilis was studied. The polysaccarides of Fomes Fomentarius and Kuehneromyces mutabilis were extracted and purified by various chromatograpies. The anti-cancer effect from separated and refined fraction was confirmed by in vitro and in vivo experiments. As a result of the experiment, it is confirmed that the separated and refined fraction has similar or better component than PS-K(anti-cancer medicine) that is already on the market</sub>

목차 Contents

• 제출문 ... 1
• 요약문 ... 2
• SUMMARY ... 14
• CONTENTS ... 27
• 목차 ... 28
• 제1장 연구개발과제의 개요 ... 29
• 제1절 연구개발의 목적 ... 29
• 제2절 연구개발의 필요성 ... 29
• 제3절 연구개발의 범위 ... 30
• 제2장 국내외 기술개발 현황 ... 33
• 제3장 연구개발수행 내용 및 결과 ... 34
• 세부1 기능성 우수 균주 육성 및 인공 재배 기술 개발 ... 34
• 제1절 기능성버섯 균주 수집 및 유연관계 ... 34
• 제2절 기능성버섯의 재배법 개발 ... 53
• 제3절 기능성버섯 품종육성 ... 69
• 제4절 기능성 관련 표지형질 ... 84
• 제5절 References ... 91
• 세부2 유효성분의 기능성 연구를 통한 생물소재화 - 버섯으로부터 혈전증 치료 물질의 개발 및 기능성 제품 생산 연구 ... 92
• 1. 버섯류로부터 혈소판 응집억제 물질과 혈전 용해물질의 탐색 ... 92
• 2. Inonotus obliquus ASI 74006이 생성하는 혈소판 응집 억제 물질의 정제 및 특성 ... 95
• 3. 정제된 혈소판 응집저해 물질의 항혈전 작용 ... 98
• 4. 혈전증 예방용 차가버섯 음료의 제조 및 특성 ... 98
• 참고문헌 ... 100
• 위탁1 : 버섯으로부터 고혈압예방을 위한 안지오텐신 전환효소(ACE) 저해물질의 개발 및 기능성 제품 생산 연구 ... 102
• 1. 항고혈압활성을 가진 버섯의 선별 및 ACE 저해물질의 추출최적조건 ... 102
• 2. Pholiota adiposa ASI 24012가 생성하는 ACE 저해물질의 정제 및 특성 ... 108
• 3. 고혈압 예방용 비늘버섯 제품의 제조 및 특성 ... 111
• 참고문헌 ... 115
• 위탁2 버섯류 천연 항산화물질 분석 및 활성물질 분리·동정 연구 ... 117
• 제1절 고 항산화 활성 버섯류 선발 및 활성물질 분리·동정 연구 ... 117
• 참고문헌 ... 168
• 협동1 버섯류의 면역조절기능 물질의 탐색 및 이용 연구 ... 172
• I. 버섯류 면역 조절 기능물질의 탐색이용 연구 ... 172
• 결론 ... 190
• 고찰 ... 191
• 참고문헌 ... 192
• 협동2 고부가 가치성 버섯 균사체의 대량 생산 연구 ... 197
• 제1절 연구내용 ... 197
• 제2절 연구결과 ... 202
• 참고문헌 ... 226
• 협동3 : 무리우산버섯 및 말굽버섯의 개발 및 이용 연구 ... 228
• 말굽버섯의 인공재배 기술 개발 ... 228
• 제1절 서설 ... 228
• 제2절 연구수행 내용 및 방법 ... 229
• 제3절 연구결과 ... 235
• 제4절 요약 ... 244
• 무리우산버섯의 인공재배기술 개발 ... 245
• 제1절 서설 ... 245
• 제2절 연구개발수행 내용 및 방법 ... 246
• 제3절 연구결과 ... 249
• 제4절 요약 ... 258
• 무리우산버섯과 말굽버섯의 기능성연구 ... 259
• 가. 연구개발수행 결과 ... 259
• 참고문헌 ... 266
• 제4장 목표달성도 및 관련분야에의 기여도 ... 269
• 세부1 기능성 우수 균주 육성 및 인공 재배 기술 개발 ... 269
• 세부2 유효성분의 기능성 연구를 통한 생물 소재화 ... 271
• 위탁1 버섯으로부터 고혈압예방을 위한 안지오텐신 전환효소(ACE) 저해물질의 개발 및 기능성 제품 생산 연구 ... 272
• 위탁2 버섯류 천연 항산화물질 분석 및 활성물질 분리·동정 연구 ... 273
• 협동1 버섯류의 면역조절기능 물질의 탐색 및 이용 연구 ... 277
• 협동2 고부가 가치성 버섯 균사체의 대량 생산 연구 ... 278
• 협동3 무리우산버섯 및 말굽버섯의 개발 및 이용 연구 ... 279
• 제5장 연구개발결과의 활용계획 ... 280
• 세부1 기능성 우수 균주 육성 및 인공 재배 기술 개발 ... 280
• 세부2 유효성분의 기능성 연구를 통한 생물 소재화 ... 280
• 위탁1 버섯으로부터 고혈압예방을 위한 안지오텐신 전환효소(ACE) 저해물질의 개발 및 기능성 제품 생산 연구 ... 281
• 위탁2 버섯류 천연 항산화물질 분석 및 활성물질 분리·동정 연구 ... 281
• 협동1 버섯류의 면역조절기능 물질의 탐색 및 이용 연구 ... 282
• 협동2 고부가 가치성 버섯 균사체의 대량 생산 연구 ... 283
• 협동3 무리우산버섯 및 말굽버섯의 개발 및 이용 연구 ... 283
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 284
• 제1절 제2회 극동아시아 식용균 공동연구 세미나 참석(2002년 9월 4일 ~ 2002년 9월 9일) ... 284
• 제2절 제2차 국제약용버섯 학술회의 참가 발표(2003년 7월 15일 - 2003년 7월 22일) ... 288
• 제3절 제16차 국제식용균 학술회의 참가 발표(2004년 3월 13일 - 2004년 3월 20일) ... 292
• 끝페이지 ... 295