초록 ▼

○ 연구결과
1) 옥수수의 내염성 특성을 알아보고자 염류처리된 수경재배를 실행한 결과 염처리 농도가 높을수록 또한 시일이지 나면 서식물체의 광합성율과 삼투압 포텐셜은 저하하였으나, 유리proline 함량은 증가 하였다.
2) 환경조절실에서 염류 집적 포트에 Cleaning crop의 재배를 실행한 결과 좋은 수분상태에 서식물의 광합성율이 높았을 뿐만 아니라 제염 효과도 더 좋았다.
3) 염류집적 중북부 지역 춘천시설 재배지 미사질식양토에서 sudan grass의 재배를 실행한 결과, 밀식인 10 × 10 cm에서 소

○ 연구결과
1) 옥수수의 내염성 특성을 알아보고자 염류처리된 수경재배를 실행한 결과 염처리 농도가 높을수록 또한 시일이지 나면 서식물체의 광합성율과 삼투압 포텐셜은 저하하였으나, 유리proline 함량은 증가 하였다.
2) 환경조절실에서 염류 집적 포트에 Cleaning crop의 재배를 실행한 결과 좋은 수분상태에 서식물의 광합성율이 높았을 뿐만 아니라 제염 효과도 더 좋았다.
3) 염류집적 중북부 지역 춘천시설 재배지 미사질식양토에서 sudan grass의 재배를 실행한 결과, 밀식인 10 × 10 cm에서 소식인 20 × 20 cm 보다 광합성 율이 낮게 나타났지만 식물체 간의 영양성분의 경쟁흡수로 인하여 밀식인 10 × 10 cm에서 소식인 20 × 20 cm에 비해 토양 염류제거 효과가 높았다.
4) 염류집적 시설재배지에서 찰옥2호의 재배를 실행한 결과, 재식거리 30 × 30 cm에서 다른 재식거리보다 광합성율도 높았고 생육상태도 좋았으며 토양의 염류제거 효과도 높았다.
5) 염류집적 시설재배지에서 후작물 오이의 재배를 실행한 결과, Cleaning crop의 재배에 의해 토양 염류를 제 거한구역에서Cleaning crop를 재배하지 않은 구역 보다 오이의 광합성율이 높게 나타났고, 생육 상태도 좋았다.
6) 염류집적 중남부지역(영주) 시설재배지(사양토)에서 Cleaning crop의 재배를 실행한 결과 식물체의 광합성율 수 분포텐셜은 saline 처리구에서 nonsaline 처리구에 비해 낮게 나타났다 Cleaning crop의 재배에 의한 토양염류를 제거하고 후작물 배추, 호밀을 재배한 결과 염류에 의한 피해를 받지 않았다.

Abstract ▼

III. Research Results and Suggestions for Application
1. Mechanism of salt tolerance for cleaning crop
The hydroponic cultivation with 0, 50, and 100 mM NaCl to understand salt tolerance of corn produced the following results: there was no significant difference between 0 and 50 mM NaCl cultiv

III. Research Results and Suggestions for Application
1. Mechanism of salt tolerance for cleaning crop
The hydroponic cultivation with 0, 50, and 100 mM NaCl to understand salt tolerance of corn produced the following results: there was no significant difference between 0 and 50 mM NaCl cultivation in the photosynthesis rate during the seedling period while the 100 mM NaCl cultivation gradually decreased in the rate 1, 4, and 7 days after saline treatment by 76%, 49%, and 31%, respectively, compared with non-saline treatment. The osmotic potential during the corn seedling period decreased drastically as the NaCl concentration increased; meanwhile, the free proline content on the ground increased remarkably as the NaCl concentration increased and time went by.
Besides, there was a significantly positive relationship between the osmotic potential and photosynthesis rate while there was a highly negative relationship between the osmotic potential and free proline content. Salt tolerant corn seedlings could be used as the useful application of transplanting materials in salt accumulation soil of the plastic film house cultivation.
2. Mechanism of salt tolerance in cleaning crops in the plant environmental control chamber
After cultivating cleaning crops, sudan grass and Chalok-2, in the salt accumulation port in the plant environmental control chamber through overhead flooding control, both of the crops showed a higher photosynthesis rate, a higher salt content within a plant, and a more effective salt removal in the treatment port with a good water condition than in the port with water stress. After examining the difference between Chunchon-jaerae and Yangjeul memil in the photosynthesis rate and rutin content by saline treatment, the photosynthesis rate decreased as the NaCl concentration increased in comparison with both of buckwheats while it is a little higher in 5 mM NaCl treatment than in non-saline cultivation. The rutin content also decreased as the NaCl concentration increased while that in a leaf of Chunchon-jaerae memil is higher in 5 mM and 10 mM treatments by 1.3 and 1.4 times, respectively, than non-saline treatment.
3. Development of salt removal technology using cleaning crops in salt accumulation soil of the plastic film house cultivation
1) Analysis of succeeding-crop cabbage, and effect of salt removing of Chalok-2 according to the different planting distance cultivation
The salt accumulation in corn cultivation soil according to the planting distance decreased remarkably as time went by; the salt content in soil decreased by approximately 30 to 50% by 50 days after transplantation. The spacious planting culture is higher in the photosynthesis rate of corn according to the planting distance than the dense planting culture; the salt content in a plant according to the number of days is generally highest by 30 days after transplantation, particularly with a higher K⁺ content than other kinds of salt. The succeeding-crop cabbage showed no difference in a photosynthesis rate and a salt content according to the planting distance while it showed higher dry and fresh weight in 25×25cm and 40×40cm planting distances than in the non-saline treatment and the 55×55cm distance.
2) Physiological characteristics and effects of salt removal using sudan grass in salt accumulation soil of the plastic film house cultivation
After examining the difference in a photosynthesis rate of sudan grass according to the planting distance in the plastic film house cultivation area with salt accumulation, the intensive culture of 10×10cm showed the lower rate than the extensive culture of 20×20cm regardless of a time. It increased remarkably in both of the planting distances as time went by.
The dense planting culture of 10×10cm showed a serious growing difficulty at the early stage of growth; however, it increasingly showed a decrease in the difference from other planting distances as time went by.
The salt content in the plant body was higher in the dense planting culture of 10×10cm than in the spacious planting culture of 20×20cm; the K+ content is much higher than other kinds of salt almost regardless of the planting distance; and the content is higher on the ground than underground. After examining effectiveness of salt removal from soil according to plant cultivation, the spacious planting culture of 20×20cm was ineffective while the dense planting culture of 10×10cm showed great effectiveness with a saline decrease in K⁺ by 28%, Ca⁺⁺ by 36.6%, Mg⁺⁺by 30.6%, and Na⁺ by 22.9%. The amount of salt decreased remarkably by 54 days, compared with 40 days, after transplantation, which demonstrates improvement in effectiveness of salt removal as time went by. It could be used as the useful application of salt removal technology using sudan grass cultivation.
3) Physiological characteristics and effects of salt removal technology using Chalok-2 in salt accumulation soil of the plastic film house cultivation.
After examining the difference in the photosynthesis rate of corn according to the planting distance, the distance of 40×40cm showed the lowest rate by 23 days after transplantation (May 31); however, there was increasingly no significant difference in the photosynthesis rate due to increased salt tolerance in the plant as time went by. As for the difference in growth features of a plant, the planting distance of 40×40cm showed a growing disorder due to the influence of salt by 23 days after transplantation (May 31); however, there was a desirable growth as time went by. For the difference in the salt content within a plant, the planting distance of 40× 40cm tended to be higher than other planting distances, and the K⁺ content is much higher than other kinds of salt after examining the difference in salt absorption. As for the correlation between saline components within a plant, there was a significant negative correlation among K⁺, Ca⁺⁺, and Na⁺ while there was a significant positive correlation among Mg⁺⁺, Ca⁺⁺, and Na⁺.
After examining the effectiveness of salt removal from soil according to corn cultivation, the planting distance of 30×30cm showed a remarkable decrease by 37 days, compared with 23 days, after transplantation in K⁺ by 28%, Ca⁺⁺ by 36.6%, Mg⁺⁺ by 30.6%, and Na⁺ by 22.9%. And the salt content is higher in surface soil than in subsoil.
4) Physiological characteristics of succeeding-crop cucumber and oat after cleaning crop cultivation
When cultivating succeeding-crop cucumber, the cleaning area showed a more higher photosynthesis rate and better growth with much higher fresh and dry weight, and leaf area than the non-cleaning area. The non-cleaning area showed a higher salt content in soil by 2, 2.6, 3.7, and 1.5 times for K⁺, Ca⁺⁺, Mg⁺⁺, and Na⁺, respectively, than the cleaning area. When cultivating winter ryes after a cucumber harvest, the non-cleaning area showed a higher K⁺ content by 1.3 and 1.4 times on the ground and underground, respectively, than the cleaning area. It seem to be due to the increase in salt absorption from soil through growth.
5) Comparison of effects of salt removing between cleaning crops, and characteristics of salt tolerance in Mibackchal corn
When cultivating Mibackchal corn in the plastic film house cultivation area, the free proline content of a leaf was much higher in the saline treatment than in the non-saline treatment and there was much more significant increase in the saline treatment as time went by. The photosynthesis rate, and water and osmotic potentials of a leaf were even lower in the saline treatment than in the non-saline treatment. As for growth features of cleaning crops, Chalok-2 showed no significant difference between two treatments while sudan grass and Mibackchal corn showed a serious growing problem in the saline treatment in comparison with the non-saline treatment. After examining the salt content in surface soil according to cleaning crop cultivation, Chalok-2 showed a decrease in Ca⁺⁺, K⁺, and Mg⁺ contents by 19.2%, 42.6%, and 13.2%, respectively, with higher effectiveness of salt removal than sudan grass and Mibackchal corn. It could be used as the useful application of salt removal technology using cleaning crop for cucumber cultivation in salt accumulation soil of plastic film house cultivation area.
6) Development of salt removal technology using cleaning crop in salt accumulation soil of plastic film house cultivation in middle-south area(Youngju) of Korea.
As for the photosynthesis rate, and water and osmotic potentials of a leaf of cleaning crops, all of them were lower in the saline treatment than in the non-saline treatment. Chalok-2 showed the highest photosynthesis rate in the saline treatment. After examining the difference in the salt content within a plant of cleaning crops, all of Mibackchal, Chalok-2, and sudan grass showed a higher content in the saline treatment than in the non-saline treatment. The K⁺ content was much higher than other kinds of salt but decreased significantly as time went by. As for growth features of cleaning crops, all of Mibackchal, Chalok-2, and sudan grass showed a remarkable growth problem in the saline treatment, compared with the non-saline treatment. After examining the difference in the salt content in surface soil according to cleaning crop cultivation, Chalok-2 showed a decrease in Ca⁺⁺, K⁺, and Mg⁺ contents by 25%, 54%, and 30%, respectively, demonstrating higher effectiveness of salt removal than sudan grass and Mibackchal corn.
When cultivating succeeding-crop cabbage, there was no difference between the non-saline and saline treatments for Chalok-2 and Mibackchal cultivation, which demonstrates similarity to the non-saline treatment through salt cleaning in saline soil. However, the saline treatment port for sudan grass cultivation showed lower effectiveness than the non-saline treatment, which seemed to be related to the fact that sudan grass has the lowest effectiveness of salt removal from saline soil and thus revealed a higher remaining salt content than in other areas. When cultivating winter ryes after a cabbage harvest, the difference in fresh and dry weight demonstrated the same result with cabbage. It is good results of salt removed using cleaning crop in salt accumulation soil of the plastic film house cultivation in middle-south area(Youngju) of Korea, it could be used as the useful application of salt removal technology in this area.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• Summary ... 9
• CONTENTS ... 16
• 목차 ... 22
• 제1장 서론 ... 27
• 제1절 연구개발의 필요성 ... 27
• 제2절 연구개발의 목적과 범위 ... 28
• 제3절 연구 개발의 목적과 범위 ... 28
• 제2장 Cleaning crop의 내염성 대사 구명 ... 31
• 제1절 서론 ... 31
• 제2절 재료 및 방법 ... 33
• 1) 발아율 측정 ... 33
• 2) 수경재배 ... 33
• 3) 식물 재료 ... 33
• 4) 광합성측정 ... 33
• 5) 삼투 포텐셜측정 ... 34
• 6) 유리 Proline 함량 측정. ... 34
• 7) 식물체 염류성분 분석 ... 34
• 제3절 연구결과 및 고찰 ... 35
• 1) Cleaning Crop 및 일반 작물의 염류농도별 발아생장 분석 ... 35
• 2) 수경재배시 Cleaning Crop 및 일반 작물의 염류농도별 내염성 특성 분석 ... 38
• 3)찰옥2호 수경재배시 염류농도별 내염성 특성 ... 40
• 제4절 결 과 요 약 ... 49
• 제3장 식물환경조절실에서 Cleaning crop의 내염성 특성 ... 50
• 제1절 서론 ... 50
• 제2절 재료 및 방법 ... 52
• 1) 식물 재료 및 토양 염류 조건 ... 52
• 2) 식물체 및 토양 염류성분 분석 ... 53
• 3) 광합성율의 측정 ... 53
• 4) Rutin 함량분석 ... 53
• 제3절 결과 및 고찰 ... 54
• 1) 염류축적 재배 Box에서 관수조절에 의한 sudan grass의 재배에 따른 토양 염류함량의 변화 ... 54
• 2) 염류축적 재배 Box에서 관수조절에 의한 찰옥2호의 재배에 따른 토양염류함량의 변화. ... 55
• 3) 염류축적 재배 Box에서 관수조절에 의한 sudan grass의 광합성율의 차이. ... 56
• 4) 염류축적 재배 Box에서 관수조절에 의한 찰옥2호의 광합성율의 차이. ... 57
• 5) 염류축적 재배 Box에서 관수조절에 의한 sudan grass의 식물체의 염류 함량의 차이. ... 58
• 6) 염류축적 재배 Box에서 관수조절에 의한 찰옥수수의 식물체의 염류함량의 차이. ... 59
• 7) 염류축적 재배 Box에서 토마토의 생장분석과 광합성 차이 ... 60
• 8) 염류축적 재배 Box에서 메밀의 광합성과 rutin 함량의 차이 ... 64
• 제4절 결 과 요 약 ... 65
• 제4장 염류 집적 시설재배지에서의 Cleaning Crop에 의한 염류 제거 기술개발 ... 67
• 제1절 서론 ... 67
• 제2절 재식거리에 따른 제염효과 및 후작물 배추의 생장분석 ... 70
• 1. 재료 및 방법 ... 70
• 1) 식물재료 및 염류토양조건 ... 70
• 2) 식물체 및 토양 염류 성분 분석 ... 70
• 3) 광합성율의 측정 ... 71
• 4) 식물체 생체중, 건물중 측정 ... 71
• 2. 결과 및 고찰 ... 71
• 1) 재식거리에 따른 토양의 염류축적 양의 변화 ... 71
• 2) 재식거리에 따른 광합성율의 차이 ... 73
• 3) 재식거리에 식물체내의 염류함량의 차이 ... 74
• 4) 후작물 배추의 광합성율의 차이 ... 77
• 5) 후작물 배추의 생체중, 건물중 및 염류축적량의 차이 ... 78
• 3. 결 과 요 약 ... 80
• 제3절. 염류가 축적된 시설재배지에서 sudan grass 재배에 따른 제염효과와 생리적특성 ... 82
• 1. 재료 및 방법 ... 82
• 1) 식물재료 및 토양염류조건 ... 82
• 2) 식물체 생체중, 건물중 및 엽면적 측정 ... 82
• 3) 토양 및 식물체 염류 성분 분석 ... 82
• 4) 광합성율의 측정 ... 83
• 5) 유효인산 및 질산태와 암모늄태 질소 함량의 측정 ... 83
• 2. 결과 및 고찰 ... 84
• 1) 염류축적 토양에서 재식거리와 시기에 따른 광합성율의 차이 ... 84
• 2) 염류축적 토양에서 재식거리에 따른 sudan grass의 생육 특성 ... 85
• 3) 염류축적 토양에서 재식거리와 시기에 따른 sudan grass의 염류축적량의 차이 ... 87
• 4) Sudan grass 재식거리와 시기에 따른 토양의 염류축적량의 변화 ... 92
• 3. 결 과 요 약 ... 95
• 제4절. 염류가 축적된 시설재배지에서 찰옥2호 재배에 따른 토양 제염효과와 생육특성 ... 96
• 1. 재료 및 방법 ... 96
• 1) 식물재료 및 토양염류조건 ... 96
• 2) 식물체 생체중, 건물중 및 엽면적 측정 ... 96
• 3) 토양 및 식물체 염류 성분 분석 ... 96
• 4) 광합성율의 측정 ... 97
• 5) 유효인산 및 질산태와 암모늄태 질소 함량의 측정 ... 97
• 2. 결과 및 고찰 ... 98
• 1) 염류축적 토양에서 재식거리와 시기에 따른 광합성율의 차이 ... 98
• 2) 염류축적 토양에서 재식거리와 시기에 따른 찰옥2호의 생육 특성 ... 100
• 3)염류축적 토양에서 재식거리와 시기에 따른 찰옥2호의 염류축적량의 차이 ... 102
• 4) 찰옥2호 재식거리와 시기에 따른 토양의 염류축적량의 변화 ... 107
• 5) 식물체내의 염류성분의 상호관계. ... 110
• 3. 결 과 요 약 ... 111
• 제5절. Cleaning crop 재배후 후작물 오이, 호밀의 생육특성 ... 112
• 1. 재료 및 방법 ... 112
• 1) 식물재료 및 토양염류조건 ... 112
• 2) 식물체 생체중, 건물중 및 엽면적 측정 ... 112
• 3) 토양 및 식물체 염류 성분 분석 ... 112
• 4) 광합성율의 측정 ... 113
• 5) 유효인산 및 질산태와 암모늄태 질소함량의 측정 ... 113
• 2. 결과 및 고찰 ... 114
• 1) 후작물 오이의 광합성율의 차이 ... 114
• 2) 후작물 오이의 생육특성의 차이 ... 115
• 3) 후작물 오이의 재배에 따른 토양 염류함량의 차이 ... 116
• 4) 겨울 호밀을 재배했을 때 식물체내의 염류함량의 차이 ... 117
• 5) 겨울 호밀을 재배했을 때 토양의 염류함량의 차이 ... 118
• 3. 결과 요약 ... 119
• 제6절 미백찰 옥수수의 내염성 특성과 cleaning crop의 제염효과 비교 ... 120
• 1. 재료 및 방법 ... 120
• 1) 식물재료 및 토양염류조건 ... 120
• 2) 토양 및 식물체 염류 성분 분석 ... 120
• 3) 광합성율 및 엽록소 함량의 측정 ... 121
• 4) 삼투 포텐셜 측정 ... 121
• 5) 유리 proline 함량 측정 ... 121
• 6) 식물체 생체중, 건물중 및 엽면적 측정 ... 121
• 2. 결과 및 고찰 ... 122
• 1) 미백찰 옥수수 재배에 따른 토양 염류함량의 차이 ... 122
• 2) 미백찰 옥수수 잎의 유리 proline 함량의 차이 ... 122
• 3) 미백찰 옥수수 잎의 수분, 삼투압 포텐셜의 차이 ... 124
• 4) 미백찰 옥수수 잎의 엽록소 함량의 차이 ... 125
• 5) 잎의 광합성율의 차이 ... 126
• 6) 미백찰 옥수수잎의 염류함량의 차이 ... 127
• 7) 미백찰, 찰옥2호 및 sudan grass 재배 따른 토양 염류축적의 변화 ... 130
• 8) Cleaning crop들의 생육특성의 차이 ... 130
• 3. 결 과 요 약 ... 131
• 제7절. 염류집적 중남부지역(영주) 시설재배지에서의 Cleaning Crop에 의한 염류제거 기술개발 ... 133
• 1. 재료 및 방법 ... 133
• 1) 식물재료 및 토양염류조건 ... 133
• 2) 식물체 생체중, 건물중 및 엽면적 측정 ... 133
• 3) 토양 및 식물체 염류 성분 분석 ... 133
• 4) 광합성율의 측정 ... 134
• 5) 수분 포텐셜 측정 ... 134
• 6) 유효인산 및 질산태와 암모늄태 질소 함량의 측정 ... 134
• 2. 결과 및 고찰 ... 135
• 1) 미백찰, 찰옥2호, sudan grass 재배 따른 토양 염류축적의 변화 ... 135
• 2) Cleaning corp들의 광합성율의 차이 ... 137
• 3) Cleaning crop들의 water potential 차이 ... 138
• 4) Cleaning crop 재배에 따른 유효인산, 암모늄태와 질산태 질소 함량의 차이 ... 140
• 5) Cleaning crop들의 생육특성의 차이 ... 143
• 6) Cleaning crop들의 식물체내의 염류 축적량의 차이 ... 146
• 7) 후작물 배추의 생체중과 건물중의 차이 ... 158
• 8) 겨울 호밀의 생체중과 건물중의 차이 ... 158
• 3. 결 과 요 약 ... 159
• 인 용 문 헌 ... 161
• 끝페이지 ... 168