초록

○ 연구결과
1. 독성해파리의 형태 및 분자생물학적 분류
2. 노무라입깃해파리 독소의 작용기작 및 중독시 제어방안 연구
3. 노무라입깃해파리 독소의 작용기작 및 중독시 제어방안 연구
4. 노무라입깃해파리의 생태학적 특성을 기초로 한 미생물 분리,배양 및 활성성분 분리 정제 및 화학구조 규명

Abstract ▼

Recent jellyfish blooming in the oceans of Korea, China, and Japan has raised a large social, economical, and medical problems, including fishery industries and health hazards to sea bathers in beaches. Despite the importance of their outbreaks, the study of these jellyfishes has been quite limited

Recent jellyfish blooming in the oceans of Korea, China, and Japan has raised a large social, economical, and medical problems, including fishery industries and health hazards to sea bathers in beaches. Despite the importance of their outbreaks, the study of these jellyfishes has been quite limited in these areas, especially in Korea. The purposes of the present study were 1) to characterize classify these jellyfishes, 2) characterize the toxicity of jellyfish venoms, and 3) investigate biological active compounds derived from the jellyfishes.
For this, we have captured various kinds of jellyfishes from the oceans near Korean peninsula. We have performed morphological and genetical analyses for these jellyfishes and compared them with those of other countries. These results shall be used for the determination of jellyfishes encountered in the field. To acquire the informations on jellyfish venoms and treat the patients poisoned by jellyfish stings, we established and investigated the in vitro and in vivo toxicity of various kinds of jellyfish venoms. We discovered the venom of N. nomurai has a greater cytotoxicity in cardiac myocytes than in skeletal muscle cells, suggesting the comparatively selective toxicity of the jellyfish venoms in heart. In addition to cytotoxicity assay, we developed LD5H method, which may allow the estimation of comparative toxicity of toxicants (or venoms), that requires only a small amount than classical LD50 method. We have also determined the characteristics of cardiac
toxicity of N. nomurai jellyfish venom, in which the venom suppressed cardiac output, resulting in hypotension. All the jellyfish venoms tested exhibited some levels of hemolytic activity in all the animal species tested with species-dependent variable potencies. In addition, we, for the first time, discovered the identities of various kinds of proteolytic enzymes in jellyfish venoms. These proteases appeared to play some important roles in the venom toxicities, such skin necrosis. For devising any beneficial use of jellyfish byproducts, we extracted, isolated, and determined the chemical structures of various biologically active compounds from N. nomurai jellyfish sample. From this, we identified 21 compounds, including 3 novel structures. Also, we could isolate microorganisms (12 kinds of marine bacteria and 12 kinds of marine fungi) from N. nomurai jellyfish sample. Some compounds derived from these demonstrated strong antibacterial activities and others showed anticancer activities.
Considering the global warming and the increase of ocean temperature, the blooming of jellyfish near Korean oceans are expected prevailing for the near future and we need further investigation on the jellyfishes. From the study, we can build a biological database of marine-derived natural resources which can be used for biological, pharmaceutical, and marine sciences.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 10
• CONTENTS ... 11
• 목 차 ... 12
• 제 1 장 연구개발과제의 개요 ... 13
• 제 1 절 연구개발의 목적 ... 13
• 제 2 절 연구개발의 필요성 ... 14
• 제 2 장 국내외 기술개발 현황 ... 17
• (1) 국내기술동향 ... 17
• (2) 국외기술동향 ... 17
• (3) 향후 전망 ... 18
• (4) 기술동향 분석 결론 ... 19
• 제 3 장 연구개발수행 내용 및 결과 ... 20
• 제 1 절 국립수산과학원 (제2협동기관) ... 20
• 제 2 절 경상대학교 (주관기관) ... 52
• 제 3 절 부산대학교 (제1협동기관) ... 86
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 107
• 1. 최종목표 ... 107
• 2. 1차년도 연구사업 ... 107
• 2. 2차년도 연구사업 ... 108
• 3. 3차년도 연구사업 ... 110
• 제 5 장 연구개발 성과 및 성과활용 계획 ... 112
• 가. 연구개발결과의 활용방안 ... 112
• 나. 기대성과 ... 112
• 다. 관련 후속연구개발의 전망 ... 113
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 114
• 제 7 장 참고문헌 ... 115
• 끝페이지 ... 115