초록

○ 연구결과
(1)연구사업 추진 결과물
(가)표고버섯 연중속성재배 시설 모델 확정 및 재배기술 안정화
(나)기존 재배품종 유연관계 분석을 통한 신품종 육성
(다)표고버섯 성분분석을 통한 가공적성 연구 및 표고버섯이용 가공상품 개발
→ 표고버섯 매생이죽(유동식)
(2)지적재산권 확보
(가)속성 표고버섯 배지 재배 방법(출원번호 :제10-2011-0134971호)외 3건
(나)표고버섯 배지재배 전용 신품종 개발(2품종 :선우,선형)

Abstract ▼

<1st subjrct : Development of low-cost, Lentinus edodes year-round properties>
According to shortage of wokers due to rural-aging and depletion of lentinus edodes wood resource, lentinus edodes culture medium cultivation has attracted much attention lately. However, diversity of lentinus edodes c

<1st subjrct : Development of low-cost, Lentinus edodes year-round properties>
According to shortage of wokers due to rural-aging and depletion of lentinus edodes wood resource, lentinus edodes culture medium cultivation has attracted much attention lately. However, diversity of lentinus edodes cultivation method by region and farm is causing confusion for farmers.
Considering of this reality of lentinus edodes culture medium cultivation, it is the object of this study in establishment of stable lentinus edodes culture medium cultivation method by development of enviornmental control optimization model of cultivation-facility for lentinus edodes culture medium cultivation and standardization of culture medium control and cultivation season
Establishment of optimization model of cultivation facility for lentinus edodes a year-long short cultivation Panel-type cultivation facility has the advantage that you can control internal environment as you want and minimize effect of external environment. Also, Panel-type cultivation facility makes it possible to cultivate lentinus edodes a year-long short time by control temperature and humidity, ventilation and apply to cultivation method more reliably
Panel-type cultivation facility model is arched urethan, the size of 7.2m×14m(100㎡). It is installed wide window in 2m×1m and two air conditioner in 5HP and electric boiler and ventilation facility, auto-control system of temperature, humidity, CO₂ and internet control system to control internal environment anywhere.
Rain shelter-type cultivation facility has been influenced by external environment.
The Cultivation facility model to develop through this study minimizes influence on external environment and make it possible to stable culture cultivation.
The scale of cultivation facility is 7.2m×14m(100㎡형), external house is covered with shading net to prepare against high temperature in summer by blocking light and ventilation-inducing. The internal house is covered with multi-layer vinyl and cashmere to insulate, installed peak on top to lower internal temperature in summer season. And external shading net is opened and closed fully top as well as side to raise temperature in winter by flood light and improve safety by minimize area of heavy snow.
Conditions of short cultivation analysis of environmental research
It is confirmed that Panel-type cultivation facility does not have been influenced by external environment and implement environment condition to set itself.
Panel-type cultivation facility model make it possible to control temperature uniformly to key to mushroom growth and cultivate all the year round.
Rain shelter-type cultivation facility is set to similar to external temperature. It makes it possible to cultivate lentinus edodes reliably. Especially low-cost lentinus edodes rain shelter cultivation season model is suggested to grow more stable because of much influence on culture in hot and humid summer.
Study lentinus edodes short cultivation condition
It is suggested to cultivaion method to move up the first harvest time dramatically to shorten culture-browning time that take a long time to culture medium cultivation and cause a failure to culture
Culture material is sterilized in high pressure stem and then inoculated liquid spawn in the primary culture, take out the bottle, molded in the shape of the box(45cm×45cm) And then it is performed on the secondary culture. After The secondary culture is completed, bring in cultivation facility, set temperature-humidity for spawn coating, and then spray water high pressure to bring browning culture medium in watering-shock. This process make it take 35-days from spawn inoculation to browing time after sterilization
<1st cooperative project : Year-round property selection breeding varieties for planting Lentinus edodesnine people and culture study>
'Seonu' 'Seonhyeong ', two breeding lines of Lentinula edodes was developed for the bag culture. One has brown color and the yield was 312.0g/1.5kg, 802.6g/7kg in the bag and box culture, and the other is dark chocolate color and the yield was 240.6g per 1.5kg. The cross and selection for advanced lines had been done by the monospore crossing method during 2008-2010. The breeding lines were evluated for yield and fruit body characteristic at three times in cultivation under structure.
Key words : Lentinula edodes, bag culture
<2st cooperative project : Lentinus edodes cultivation and processing quality characteristics year round property research and development products>
The major free sugars of Lentinus edodes were glucose and maltose detected by HPLC.
And the contents of free sugars from cultivated L. edodes were high in orders of lumber media cultivation (10.07%), sawdust media cultivation (7.17%), forcing sawdust media cultivation (6.97%) and Chinese L. edodes.
As the results of analysis of organic acids according to the cultivation methods were detected four major organic acids such as oxalic acid, malic acid, citric acid and succinic acid. The forcing cultivation media of L. edodes was the highest content of organic acid among the samples.
The result of total amino acid analysis, sixteen amino acids were detected by AccQ tag method, and the highest total amino acids content was found in sawdust media cultivation of L. edodes. The major total amino acids of forcing sawdus media cultivation were determined glutamic acid (1,029.54 mg%), arginine (867.24 mg%), valine (747.68 mg%), serine (729.22 mg%) and lysine (721.98 mg%).
The forcing cultivation of L. edodes and chinese L. edodes were showed the higher free amino acids than the other samples. But forcing sawdust media cultivation has more essential amino acids content than chinese L. edodes. The major free amino acid of forcing sawdust media cultivation of L. edodes were histidine (348.90 mg%), glutamic acid (167.40 mg%), lysine (143.62 mg%) and aspartic acid (134.94 mg%) in this test.
The major minerals of L. edodes were potassium, magnesium and sodium. The content of potassium was the highest in forcing sawdust media cultivation L. edodes, but the other mineral were not significant in this test.
Five volatile compounds and seven volatile compouns were detected in forcing sawdust media cultivation and lumber media cultivation, respectively. The major volatile compound of samples were 1-octen-3-ol determined by GC-MS. The highest content of 1-octen-3-ol was found in forcing sawdust media cultivation L. edodes.
The content of β-glucan of L. edodes was high in the orders of lumber media cultivation (42.86%), sawdust media cultivation (40.96%), forcing sawdust media cultivation(36.52%) and chinese L. edodes A, B(29.14%, 26.86%).
The polyphenol content, DPPH free radical scavenging activity and SOD-like activity of samples were not significant in this test.
Hunter's value of soup mixed L. edodes cultivated with forcing sawdust media cultivation and Capsosiphon fulvescens, L (lightness) value was not significnat in this test. But a (redness) value and b (yellowness) were incresed by C. fulvescens amount. The viscosity of soup was decreased by C. fulvescence added increased.
The result of sensory test, the highest color and texture were scored in 20% C. fulvescence added soup. The highest scores of smells and tastes were recorded in 10% and 15% C. fulvescence added soups. As the result of overall sensory evaluation, 10% added C. fulvescence soup got the highest score among the samples.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 17
• CONTENTS ... 20
• 목 차 ... 24
• 제1장 연구개발과제의 개요 ... 27
• 1. 연구개발의 목적 ... 27
• 2. 연구개발의 필요성 ... 27
• 3. 연구개발의 범위 ... 28
• 4. 기대효과 ... 28
• 제2장국내외 기술개발현황 ... 29
• 1. 국내·외 기술동향 및 수준 ... 29
• 2. 국내·외 시장규모 및 수출·입 현황 ... 31
• 3. 관련 기술/제품의 국내․외 지식재산권(특허 등) 현황 ... 33
• 제3장 연구개발수행 내용 및 결과 ... 38
• <제 1세부과제 : 저비용 표고버섯 연중속성재배 기술개발> ... 38
• 제1절 표고버섯 연중속성재배 시설 최종 모델 확립 ... 38
• 1. 머리말 ... 38
• 2. 표고버섯 시설재배 실태 현장조사 ... 38
• 3. 현장조사 분석 결과 및 시험재배사 설치 ... 43
• 4. 결론 ... 46
• 제2절 속성재배 조건별 배양 생육단계별 환경(온․습도)조사 분석 ... 50
• 1. 머리말 ... 50
• 2. 대상 및 방법 ... 50
• 3. 시험재배사 온․습도 변화 ... 51
• 4. 결론 ... 52
• 제3절 표고버섯 속성재배 조건 구명(50일) ... 54
• 1. 머리말 ... 54
• 2. 재료 및 방법 ... 54
• 3. 결론 ... 56
• 4. 연구사업 결과물 활용 ... 57
• <제1협동과제 : 연중속성 재배용 표고버섯 품종선발 육종 및 배지구명> ... 62
• 제1절 표고버섯 유전자원 수집 및 보존 ... 62
• 1. 국내외 톱밥재배용 표고버섯 균주수집 ... 62
• 2. RAPD를 이용한 표고버섯 유연 관계 분석 ... 62
• 3. 결과 및 고찰 ... 62
• 제2절 RAPD를 이용한 표고버섯 유연관계 분석 ... 66
• 1. 표고버섯 우량균주의 배양적 특성 ... 66
• 2. 표고버섯 우량균주의 자실체 특성 ... 66
• 3. 결과 및 고찰 ... 66
• 제3절 톱밥재배에 적합한 품종선발 교배육종 ... 68
• 1. 톱밥재배에 적합한 품종 선발 ... 68
• 2. 교배 및 우량균주 선발 ... 69
• 3. 결과 및 고찰 ... 69
• 4. 적요 ... 72
• 제4절 톱밥재배에 적합한 우량 균주 자실체 특성검정 ... 73
• 1. 시험균주 및 종균제조 ... 73
• 2. 배지제조 및 접종 ... 73
• 3. 배양 및 생육관리 ... 73
• 4. 결과 및 고찰 ... 73
• 5. 적요 ... 80
• 제5절 톱밥재배에 적합한 배지조성 구명 ... 81
• 1. 시험균주 및 종균제조 ... 81
• 2. 배지재료 제조 및 이화학성 분석 ... 81
• 3. 배양 및 생육 특성 조사 ... 81
• 4. 생육관리 및 생육조사 ... 81
• 5. 결과 및 고찰 ... 82
• <제 2협동과제 : 연중속성재배 표고버섯 품질특성 및 가공상품 연구개발> ... 84
• 제1절 서론 ... 84
• 제2절 표고버섯 성분분석 ... 91
• 1. 재료 및 방법 ... 91
• 2. 성분분석 방법 ... 93
• 3. 결과 및 고찰 ... 95
• 제3절 표고버섯 성분분석(2차) 및 가공식품 개발 ... 112
• 1. 재료 및 방법 ... 112
• 2. 성분분석 방법 ... 113
• 3. 결과 및 고찰 ... 116
• 4. 표고버섯, 매생이를 이용한 유동식 개발 ... 124
• 5. 표고버섯, 매생이를 이용한 유동식 개발 결과 및 고찰 ... 127
• 제4절 표고버섯 성분분석(3차) 및 최종제품 개발 ... 138
• 1. 성분분석 ... 138
• 2. 표고버섯매생이죽 품질개선 ... 152
• 3. 제품 디자인 개발 ... 156
• 제4장 목표달성도 및 관련분야에의 기여도 ... 180
• 1. 목표 달성도 ... 180
• 2. 관련분야 기여도 ... 181
• 제5장 연구개발 성과 및 성과활용 계획 ... 183
• 제1절 실용화·산업화·기술확산 계획(기술실시 등) ... 183
• 1. 사업화 현황 ... 183
• 2. 기술확산 계획 ... 184
• 제2절 교육·지도·홍보 활동 ... 185
• 1. 교육 및 지도활동 ... 185
• 2. 홍보 ... 189
• 제3절 특허, 품종, 논문 등 지식재산권 확보계획 ... 190
• 1. 특허성과 ... 190
• 2. 품종등록 ... 190
• 3. 논문게제 성과 ... 190
• 4. 국내 및 국제 학술대회 발표 ... 191
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 192
• 1. 해외 표고버섯 관련 특허현황 ... 192
• 2. 해외 제품 개발 현황 ... 193
• 제7장 참고문헌 ... 194
• 끝페이지 ... 198