[보고서]김을 이용한 기능성 소재개발

남택정

보고서 정보

주관연구기관

부경대학교
Pukyong National University

연구책임자

남택정

참여연구자

최정욱 , 이민경 , 김보경 , SAEIDI SOMA

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2014-08

주관부처

해양수산부

사업 관리 기관

한국해양과학기술진흥원

등록번호

TRKO201500000617

DB 구축일자

2015-05-02

표 Photograph of Laver(Porphyra yezoensis)
표 Types and features model of domestic laver
표 Identification report of laver (National fisheries research and development institute)
표 Features of laver
표 cell type and The presence of serratus bump
표 Purification method of glycoprotein extract from Porphyra yezoensis
표 Yield, carbohydrate and protein content of the measurement results for the extract prepared by changing the extraction solvent and conditions
표 Yield, carbohydrate and protein content of the measurement results for the extract prepared by changing the extraction solvent volume
표 Yield, carbohydrate and protein content of the measurement results for the extract prepared by changing the extraction temperature
표 Experimental procedure for the concentration degree of the concentrate and the relationship at ethanol concentration of the ethanol immersion
표 The result of concentrate volume and Brix degree measure
표 The graph of concentrate volume and Brix degree measure
표 Brix degree measure and Total yield from ethanol immersion conditions
표 Total saccharide content and total protein content from ethanol immersion conditions
표 Extraction manufacturing process of primary extract and secondary extract
표 Result of Yield, carbohydrate and protein content from primary extract and secondary extract
표 Lavel of extraction conditions in experimental design
표 Experimental range and values of the independent variables in the central composite design for optimization of extraction conditions
표 Polynomial equations calculated RSM program for extraction conditions of Porphyra yezoensis
표 Predicted levels of extraction condition for the maxim responses of variables by the ridge analysis in Porphyra yezoensis
표 Comparison of lab scale and pilot scale (Yield, Total saccharide and Total protein content)
표 Pilot scale manufacturing process records
표 Graph of change at yield(1), total saccharide content (2) and total protein content(3) from extraction, purification and drying process
표 Process improvement Manufacturing Process of laver glycoprotein extract
표 Glucose(Total carbohydrate content standard) standard curve and carbohydrate content of laver glycoprotein extract
표 Bovine serum albumin(Lowry method standard) standard curve and protein content of laver glycoprotein extract
표 Comparison of the component produced by the process improvement before and after the product (Yield, Total saccharide and Total protein content)
표 The SDS-PAGE profiles of laver glycoprotein extract (coomasssie&glycoprotein staning)
표 The SDS-PAGE profiles of laver glycoprotein extract (coomasssie staning)
표 LC/MS chromatogram of laver glycoprotein extract (High resolution intact mass)
표 Molecular weight measurements for each peak at laver glycoprotein extract (High resolution intact mass)
표 Results of peptide analysis by laver glycoprotein extract of LC/MS/MS
표 Results of peptide analysis by glycoproetin staining band of LC/MS/MS
표 Amino acid composition of laver glycoprotein extract
표 Free amino acid composition of laver glycoprotein extract
표 Mono saccharide composition of laver glycoprotein extract
표 Characteristics of the material of laver extract, sediment and supernatant
표 Effects of PYGP on the celll viability in HepG2, IEC-6, Chang, Hepa 1c1c7 cells.
표 Effects of PYGP on the celll viability in RAW 264.7 cells.
표 TBARS production in RAW 264.7 cells.
표 Effect of PYGP on the activity of SOD.
표 Effect of PYGP on the activity of CAT.
표 Effect of PYGP on the activity GSH-px
표 Effect of PYGP on NO production in RAW 264.7 cells.
표 Effect of PYGP on ROS production in RAW 264.7 cells.
표 Effect of PYGP on TNF-α production in RAW 264.7 cells.
표 Effect of PYGP on Prostaglandin E2 in RAW 264.7 cells.
표 Effect of PYGP on inflammatory signal pathway.
표 Effects of PYGP on the celll viability in Hepa 1c1c7 cells.
표 Cytokine production in RAW 264.7 cells
표 Effect of PYGP on the ALT and AST levels.
표 Effect of PYGP on the LDH levels.
표 TBARS production in Hepa 1c1c7 cells.
표 Effect of PYGP on the activity of SOD.
표 Effect of PYGP on the activity of GST.
표 Effect of PYGP on the activity of GSH.
표 Phycoerythrin structure in laver glycoprotein extract
표 Cytochrome C structure in laver glycoprotein extract
표 Acyl carrier protein conformation structure in laver glycoprotein extract
표 Purification of Porphyra yezoensis.
표 Effects of Sample C on the celll viability in Chang and Hepa 1c1c7 cells.
표 MS analysis of PYGP
표 BCA protein assay reagents and test methods Photograph
표 Effects of PPY1 on the cell viability in RAW 264.7 cells
표 Effects of PPY1 on the ROS levels in RAW 264.7
표 Effect of bioactive peptide from P. yezoensis (PPY1) on the level of nitrite oxide in LPS-stimulated RAW 264.7 macrophages.
표 Effect of bioactive peptide from P. yezoensis (PPY1) on the level of pro-inflammatory mediators and cytokines in LPS-stimulated RAW 264.7 macrophages.
표 Effect of PYGP on the paw edema inflammation.
표 Effect of PYGP on the ear edema inflammation.
표 Effect of PYGP on the GOT/GPT levels.
표 Effect of PYGP on the serum total protein levels.
표 Effect of PYGP on the serum albumin levels.
표 Effect of PYGP on the serum bilirubin levels
표 Effect of PYGP on the serum total cholesterol levels.
표 Effect of PYGP on the serum TG levels.
표 Effect of PYGP on the calcium levels in rat liver.
표 Effect of PYGP on the total cholesterol levels in rat liver.
표 Effect of PYGP on the GSH activity in rat liver.
표 Effect of PYGP on the lipid peroxidation in rat liver.
표 Effect of PYGP on the GST activity in rat liver.
표 Effect of PYGP on the SOD activity in rat liver.
표 Effect of PYGP on the CAT activity in rat liver.
표 Effect of PYGP on the MAPK pathway protein expression in rat liver.
표 Effect of PYGP on the Bcl-2 family protein expression and caspase activity in rat liver.
표 Effect of PYGP on the iNOS, COX-2 protein expression in rat liver.
표 Effect of PYGP on the TG levels in rat liver.
표 Effect of PYGP on the total lipid levels in rat liver.
표 Effect of PYGP on the total cholesterol levels in rat liver.
표 Effect of PYGP on the GOT/GPT levels in rat serum.
표 Effect of PYGP on the TG levels in rat serum.
표 Effect of PYGP on the hydroxyproline in rat liver.
표 Effect of PYGP on the GSH-px activity in rat liver.
표 Effect of PYGP on the catalase activity in rat liver.
표 Effect of PYGP on the catalase activity in rat liver.
표 Effect of PYGP on the MAPK pathway protein expression in rat liver.
표 Effect of PYGP on the iNOS, COX-2 protein expression in rat liver.
표 Effect of PYGP on MAPK signal pathway.
표 Effect of PYGP on Bcl-2 family protein expression.
표 Effect of PYGP on caspase and PARP signal pathway.
표 Mortality (Group Summary)
표 Summary of Clinical Signs (Group Summary)
표 Mean body weight of male
표 Mean body weight of female
표 Macroscopic Findings (Group Summary, Male)
표 Macroscopic Findings (Group Summary, Female)
표 Toxicity test range of regarding approval of ingredients, standard and specification of Porphyra yezoensis extract
표 Intake amount of seaweeds.
표 Intake amount at foods group.
표 Optimal production process records at laver glycoprotein extract
표 Aquaculture facilities ratio by laver breed
표 Bovine serum albumin(BCA standard)' s standard curve and protein content of laver glycoprotein extract
표 Establish extract conditions of laver glycoprotein extract(scale up)
표 Report on the extracts according to the production process
표 Hazardous Material Specifications for laver glycoprotein extract(KFDA Notification) and certificate of Analysis
표 Test reports for laver glycoprotein extract heavy metal.
표 Test reports for laver glycoprotein extract pesticide residues
표 Test reports for laver glycoprotein extract nutrients
표 Hazardous Material Specifications for functional ingredients of Health function food and recognized standard specifications (KFDA Notification)
표 Maximum permissible dose from per day about laver glycoprotein extract
표 Type and functions of purification synthetic resin
표 Extraction manufacturing process from purification synthetic resin
표 Yield, carbohydrate and protein content of the measurement results for the extract prepared by changing the purification method
표 Accelerated stability testing protocol of laver glycoprotein extract
표 Accelerated stability testing result of laver glycoprotein extract
표 Long-term preservation stability testing protocol of laver glycoprotein extract
표 Long-term preservation stability testing result of laver glycoprotein extract
표 Laver glycoprotein extract liquid formulation (drinks) manufacturing prescription (in 100 mL)
표 Granule manufacturing prescription of Laver glycoprotein extract
표 Characteristics, properties and composition by laver glycoprotein extract granule
표 Formulation test of laver glycoprotein extract granules
표 Production process of granules
표 Product specifications and appearance of the final granules
표 Formulation test of laver glycoprotein extract tablet
표 Characteristics, properties and composition by laver glycoprotein extract compression
표 Characteristics, properties and composition by laver glycoprotein extract capsule
표 Production process of prototype
표 Prototype manufacturing process records according to standardization
표 Characteristics, properties and composition by laver glycoprotein extract prototype
표 Draft and completed prototype of Laver-Khan
표 Question investigation form for the consumer preference survey
표 General information about the respondents
표 Multi-response analysis about liquefied dosage form
표 Multi-response analysis about liquefied dosage form
표 Multi-response analysis about granule dosage form
표 Multi-response analysis about granule dosage form
표 Multi-response analysis about compression dosage form
표 Multi-response analysis about compression dosage form
표 Multi-response analysis about capsule dosage form
표 Multi-response analysis about capsule dosage form
표 Production cost and selling price of Laver glycoprotein extract
표 Production Status of domestic Health functional Food
표 Domestic market scale at health functional foods
표 Performance status of health functional food production by item
표 Functional health functional food Production ratio
표 Individual recognition status raw material health functional food Production ratio
표 Individual recognition status of health functional food((A): 2013 year, (B): 10 years(2004-2013))
표 Domestic Liver function improved product and side effect
표 Stability test results for each of the packaging material
표 Procedures of Trademark application and registration
표 Trademark application form of glycoprotein from Porphyra yezoensis (Laver-khan)
표 The combination design method of on-line and off-line marketing
표 Global functional food market by year.
표 Each country functional foods market.
표 Health functional food present condition on sales in USA
표 Functional foods market in japan.
표 Functional foods market in china.
표 Each country patent share in year.
표 Each country major biological resources species.

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