보고서 정보
주관연구기관 |
국립농업과학원 National Institute of Agricultural Sciences |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2015-02 |
과제시작연도 |
2014 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
과제관리전문기관 |
국립농업과학원 National Institute of Agricultural Sciences |
등록번호 |
TRKO201500010295 |
과제고유번호 |
1395035569 |
사업명 |
농업기초기반연구 |
DB 구축일자 |
2015-07-11
|
DOI |
https://doi.org/10.23000/TRKO201500010295 |
초록
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Ⅳ. 연구개발결과
○ Azadirachtin A, azadirachtin B, deacetylsalannin 및 salannin을 지표성분으로 한 님 추출물 유효성분 정량분석 기술 개발
○ Matrine 및 oxymatrine을 지표성분으로 한 고삼 추출물 유효성분 정량분석 기술개발
○ Salicylaldehyde, cinnamaldehyde 및 cinnamyl alcohol을 지표성분으로 한 계피추출물 유효성분 정량분석 기술 개발
○ Methyl disulfide, diallyl disulfide 및 dial
Ⅳ. 연구개발결과
○ Azadirachtin A, azadirachtin B, deacetylsalannin 및 salannin을 지표성분으로 한 님 추출물 유효성분 정량분석 기술 개발
○ Matrine 및 oxymatrine을 지표성분으로 한 고삼 추출물 유효성분 정량분석 기술개발
○ Salicylaldehyde, cinnamaldehyde 및 cinnamyl alcohol을 지표성분으로 한 계피추출물 유효성분 정량분석 기술 개발
○ Methyl disulfide, diallyl disulfide 및 diallyl tridulfide를 지표성분으로 한 마늘추출물 유효성분 정량분석 기술 개발
○ Matrine 및 oxymatrine을 지표성분으로 한 회화나무 추출물 유효성분 정량분석기술 개발
○ Rotenone 및 degueline을 지표성분으로 한 데리스 추출물 유효성분 정량분석 기술 개발
○ 계면활성제 함유 시료의 전처리 기술 개발
○ 유기농자재 중 화학농약 분석법 개발
○ Head-space GC/MS를 이용하여 유기농자재 중 유기용매 분석법 개발
○ 님추출물 및 이를 활용한 유기농자재의 지표성분 유효기간 검증
○ 고삼추출물 및 이를 활용한 유기농자재의 지표성분 유효기간 검증
○ 님 추출물을 주성분으로 하는 유기농자재의 환경노출 안정성 평가
○ 고삼 추출물을 주성분으로 하는 유기농자재의 환경노출 안정성 평가
Abstract
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In an environment-friendly agriculture, plant extracts have been perceived as alternatives of synthetic pesticides. The Environment-friendly Agriculture Promotion Act of Korea has approved neem, Sophora flavescens, cinnamon, garilc, Chinese scholar tree, and derris extract as a matter for the produc
In an environment-friendly agriculture, plant extracts have been perceived as alternatives of synthetic pesticides. The Environment-friendly Agriculture Promotion Act of Korea has approved neem, Sophora flavescens, cinnamon, garilc, Chinese scholar tree, and derris extract as a matter for the production of commercial biopesticides. Many commercial biopesticides containing these extracts have been marketed locally. However, the analytical method for the quality control of these biopesticides containing six plant extracts has not been studied. Marker compounds of six plant extracts were selected on the basis of their characteristics of crop protection: azadirachtin A, azadirachtin B, deacetylsalannin, and salannin from neem, matrine and oxymatrine from Sophora flavescens, cinnam aldehyde, cinnamyl alcohol, and salicyl aldehyde from cinnamon, dimethyl disulfide, diallyl disulfide, and diallyl trisulfide from garlic, matrine and oxymatrine from Chinese scholar tree, rotenone and deguelin from derris. Cartridge clean-up methods for the determination of marker compounds in biopesticides containing six plants extract were developed and validated by ultra-performance liquid chromatography (UPLC) and gas chromatography (GC). The clean-up methods were optimized with hydrophilic lipophilic balance (HLB), ENVI-Carb, and C18 solid phase extraction (SPE) cartridges for the marker compounds in biopesticides containing plants extract, and the eluates were analyzed by UPLC and GC. The developed method was validated, and the LOQ and recovery rates of marker compounds were 0.009-3.62 mg L-1 and 73.1-105.1%, respectively. Marker compounds levels in some products among the tested commercial biopesticides containing six plant extracts were assumed to be sufficient as crop protection agents. However, if we consider the practical use guideline of biopesticide, the concentration of the marker compounds would be expected to be lower than EC50 and LD50 level for crop protection, because the general guideline for field application was suggested be 100-1000 folds diluted with water. In a survey of marker compounds in commercial biopesticides containing six plant extracts, the concentration of marker compounds showed wide ranges and these results would be related to the crop protection efficiency. Thus, the quality control of the commercial biopesticides should be performed. This study successfully developed and validated the clean-up method by using HLB, ENVI-Carb, and C18 SPE cartridges for simultaneous determination of marker compounds in commercial biopesticdes containing six plant extracts, which are constituted with various interferences of instrumental analysis such as pigments, lipids, and surfactants. These methods could be contributed to the manufacture for quality control and to the method development for the quantitative analysis or effective purification of marker compounds in other biopesticides.
Organic farming materials for plant disease and pest control are mixed materials of plant extracts and various surfactants. These materials can be contaminated with the organic solvents during manufacturing. The purpose of this study was to develop the analytical method of residual volatile organic solvents in organic farming materials using the headspace sampler in combination with GC-MS. Nine solvents (acetone, dichloromethane, n-hexane, ethyl acetate, chloroform, benzene, toluene, ethylbenzene, and xylene) were selected, and recovery rate was tested in two commercial products. Sample was made with distilled water(DW) and saturated sodium chloride solution. The recovery rate of organic solvents made with DW was range from 56.8-157.3%, and made with salt solution was range from 49.4-127.8%. The recovery rate was highly affected by products composition.
The stabilities of the major active ingredient of the extracts from neem tree and S. flavescens are important factor to establish expiry date and usage manual for crop protection. However, the environmental stability of the compounds had not been studied with the extract and its commercial biopesticides. Here, the stabilities of four limonoidal substances including azadirachtin A, azadirachtin B, deacetylsalannin and salannin were investigated both in controlled aquatic and soil conditions. The half-life of the total limonoid for neem extracts and its two commercial biopesticides was estimated 86.6-173 days in water under air, while degradation of the compounds was detected below 10 % after eight weeks in deoxygenated water. The half-life in dry soil was estimated 43.3-57.7 days, and there was a similar degradation pattern with in aerobic water condition. In case of wet soil condition, the total bacteria of the soils ranged 6-8 log CFU/g soil for during the experiment, and the half-life of the total limonoid was 6.4-12.3 days. From the result, the fast limonoid degradation in wet soil environment was the result of both chemical oxidation and microbial degradation. In case of two alkaloidal substances like matrine and oxymatrine, the half-lives of the total matrines for the extract and its two commercial biopesticides were estimated over 200 days both under aerobic and anaerobic water condition. Under dry soil condition, the initial decay rates of the matrines were calculated 0.0804-0.1275 (t1/25.4-8.6days), and the half-lives under wet soil condition were calculated 33.0-231 days. Total soil bacteria on the wet soil ranged 6.0-8.0 logCFU/g-soil during the experiments period. The aquatic mixture of the extract showed excellent stability both with the extract and its biopesticides, however, the stability of soil mixture were shorter than the aquatic mixture, suggesting that soil metal consider as a catalyst for the degradation of the two alkaloids.
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