초록 ▼

□ 과제명 : 잣잎(가지포함) 추출물을 이용한 개별인정 건강식품 원료인정을 위한 연구
▶ 연구목적 :
○ 잣잎 추출물의 개별인정형 건강식품 원료로써의 가치를 규명
○ 잣잎 추출물의 이상지질혈증의 개선효과를 임상시험을 통해 규명
○ 잣잎 추출물의 이상지질혈증 개선효과의 기전 연구
○ 버려지는 부산물에서의 경제적 산업적 가치 규명
▶ 주요연구내용 :
○ 1세부과제명 : 잣나무 잎을 이용한 고지혈증 개선용 기능성 성분 원료의 건강기능제품 인정
- 잣나무 잎과 가지의 추출물의 원료연구를 통해 효율

□ 과제명 : 잣잎(가지포함) 추출물을 이용한 개별인정 건강식품 원료인정을 위한 연구
▶ 연구목적 :
○ 잣잎 추출물의 개별인정형 건강식품 원료로써의 가치를 규명
○ 잣잎 추출물의 이상지질혈증의 개선효과를 임상시험을 통해 규명
○ 잣잎 추출물의 이상지질혈증 개선효과의 기전 연구
○ 버려지는 부산물에서의 경제적 산업적 가치 규명
▶ 주요연구내용 :
○ 1세부과제명 : 잣나무 잎을 이용한 고지혈증 개선용 기능성 성분 원료의 건강기능제품 인정
- 잣나무 잎과 가지의 추출물의 원료연구를 통해 효율적 분리 방법을 표준화
- 잣잎 추출물의 비만 및 고지혈 모델에서의 기능성 평가
- 잣잎 추출물 선정과 표준화 및 지표물질 성분 확립에 대한 근거자료 제시
- 잣잎 추출물에서의 지표 물질 Lambertianic acid 확립
- Lambertianic aci의 효능평가 항비만 및 항 고지혈 효과 검정
- 동일조건 유사종의 추출물과 잣잎 추출물의 기기 분석을 통한 주요 함유성분 물질 연구
- 잣잎 추출물 제조를 위한 공장설비 기준 공정개발 (대량화)
- 잣나무 가지를 포함하는 잎 추출물의 건강기능 제품으로써의 개별인정 신청
○ 2세부과제명 : 잣잎 추출물을 이용한 이상지질혈증 개선용 기능성 및 안전성평가
- 임상시험심사위원회 IRB 승인- IRB No. KMC IRB 1227-02
- 인체적용시험 시 의학영양치료를 위한 프로토콜 마련
- 인체적용시험 대상자 모집을 위한 홍보 및 모집
-12주 대조군 병행, 무작위 배정, 인체적용시험 진행
-잣잎 추출물 군에서 VLDL 억제로 인한 이상지질혈증 개선효과

Abstract ▼

The objective of this study was to investigate the anti-hyperlipidemic effects of Pinus koraiensis leaves extract in clinical trial. Our previous studies have shown that the Pinus koreaiensis extract exerts anti-hyperlipidemmic1), anti-diabetic2), anti-obesity3) and anti-cancer effects4) in vitro an

The objective of this study was to investigate the anti-hyperlipidemic effects of Pinus koraiensis leaves extract in clinical trial. Our previous studies have shown that the Pinus koreaiensis extract exerts anti-hyperlipidemmic1), anti-diabetic2), anti-obesity3) and anti-cancer effects4) in vitro and in vivo model.
Pinus Koraienesis, generally called the korean nut pine, is an evergreen tree found in Korea, China, Japan, and eastern Russia. Unlike the common Pinaceae plants, P. koraiensis shows leaves in fascicles of five. Leaves were harvested from a korean pine tree that was more than 30 years old at the end of October in Gangwondo Honchengun where is famous for producing excellent korean pine nuts in the world.
Isolation method of Pinus koraienesis extract for industialization were standardization. Pinus koraienesis in the order to increasing the extraction efficiency, was defined leaves and youg stems under 1 cm in diameter and was cut into a length of 2-3 cm. Dried and pulverized P. koraienesis leaves were immersed in 50% Ethanol and distilled. The refulx distillation continued for 10 h at 45. The extract was prepared that 1-2times extract mixed under reduced pressure to obtain a 30 Brix. Though activity-guided fractionation, we identified lambertianic acid as a novel anti-hyperlipidemic compound for increasing LDLR.Lambertianic acid inhibited fat accumulation in adipocyte and increased LDLR.Further, we performed comparative study on constituents and anti-hyperlipidemic effect in Pinus Koraienesis leaves extract and similar species (pine needle) extract. Pinus Koraiensis extract much more contained all constituents than pine needle extract.Pinus koraiensis extract much more increased LDLR than pine needle extract.
In clinical trial, we used parallel-group trial randomly. 33 participants received PKE (n =20) or placebo (n = 13) for 12 weeks. Anthropometric indexes and blood pressure were measured every 4 week. Blood parameters (serum lipids, insulin, glucose, and antioxidants)were assessed baseline and after 12 weeks. The research protocol was approved by the institutional review board (KMC IRB 1227-02) of Kyung Hee Medical Center (Seoul,Korea). Consistantly with in vitro and in vivo data, very low-density lipoprotein cholesterol decreased significantly (from 24.4±10.0 mg/dL to 18.4±4.1 mg/dL) (P < 0.05)and superoxidase increased significantly after 12 weeks (6.12±0.41 U/mL to 9.06±0.62 U/mL) (P < 0.01) in pinus koraienesis extract treated group. Furthermore, the mean systolic blood pressure (P < 0.05) and waist circumference (P < 0.05) were also significantly reduced after the experiment compared with the baseline levels in the Pinus koraienesis extract group.
The results suggest that Pinus koraienesis extract could be beneficial effect and might be useful for subject with hyperlipidemia or metabolic diseases.