보고서 정보
주관연구기관 |
국립농업과학원 National Institute of Agricultural Sciences |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2015-02 |
과제시작연도 |
2013 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201500010777 |
과제고유번호 |
1395034700 |
사업명 |
국책기술개발 |
DB 구축일자 |
2015-07-11
|
DOI |
https://doi.org/10.23000/TRKO201500010777 |
초록
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Ⅳ. 연구개발결과
○ 제1세부과제 : 당귀의 육묘시스템 및 대량 생산 재배시스템 개발
- LED 및 형광등을 단독 조사할 수 있는 조명장치, 노즐에 의해 저면 분사할 수 있는 재배장치로 구성된 육묘시스템을 설계 제작하였다. 조명장치는 조사 높이를 10∼40cm 가변할 수 있으며, 재배장치에는 양액을 냉각할 수 있는 냉각기를 부착하여 근권부 온도를 제어할 수 있도록 하였다.
- 광 조사거리가 20cm일 때 LED 육묘시스템의 PPFD는 최대 338.4 μmol m-2s-1, 최소
Ⅳ. 연구개발결과
○ 제1세부과제 : 당귀의 육묘시스템 및 대량 생산 재배시스템 개발
- LED 및 형광등을 단독 조사할 수 있는 조명장치, 노즐에 의해 저면 분사할 수 있는 재배장치로 구성된 육묘시스템을 설계 제작하였다. 조명장치는 조사 높이를 10∼40cm 가변할 수 있으며, 재배장치에는 양액을 냉각할 수 있는 냉각기를 부착하여 근권부 온도를 제어할 수 있도록 하였다.
- 광 조사거리가 20cm일 때 LED 육묘시스템의 PPFD는 최대 338.4 μmol m-2s-1, 최소 123.5 μmol m-2s-1, 평균 246.3 μmol m-2s-1, 형광등 육묘시스템의 경우 최대 112.7 μmol m-2s-1, 최소 40.2 μmol m-2s-1, 평균 81.6 μmol m-2s-1로 나타나 중앙부가 가장자리 보다 2.7∼2.8배 정도 높아지는 경향을 보였다.
- 육묘시스템을 이용하여 30일 동안 만추당귀 종자의 발아율을 조사한 결과 초음파 5분 처리에서 75.6%, 10분 처리에서 74.4%, 15분 처리에서 81.1%로 나타나 관행 대비 36.3~43.0% 향상되었다.
- LED 및 형광등 육묘시스템을 이용하여 재배시험을 실시한 결과 초장을 제외한 엽수, 엽장, 엽폭 모두 LED 처리구에서 높게 나타나, 양호한 생육상태를 확인할 수 있었다.
- 당귀 재배시스템의 성능시험 결과 트레이 재배, 포트 재배가 가능하였으며, 엽수, 엽장, 엽폭의 생육상태도 양호한 것으로 나타났다.
○ 제1협동과제 : 당귀의 최적 환경조건 구명 및 작부체계 개발
- 당귀의 수경재배 방식은 분무경과 배지경이 적합한 것으로 나타났으며, 최적의 광 조건은 형광등 또는 적청LED, 백색 LED 광 이용시 정식 후 150-200 μmol m-2s-1 수준이었다. 또한 배양액의 온도가 22∼23℃일 때 지상부 생체중의 평균값이 높게 나타났다.
- 최적 환경조건은 PPFD 1500 μmol m-2s-1, CO2 농도 1,000 μmol mol-1, 온도 20∼26℃ 조건에서 개엽 광합성이 가장 높은 조건으로 도출되었다.
- 프라이밍 처리에 의한 발아율은 (Ca(NO3)2 0.3M 농도 처리에서 약 70%로 가장 높게 나타났다.
- 당귀의 생장은 적색, 청색, 백색 LED 처리에서 유사한 경향을 보였으며, 녹색과 황색 LED를 2주간 처리 시 Decrusin 함량이 증대되는 것으로 나타났다.
Abstract
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○ Development of seedling system and mass production system for Angelica gigas
This study was to investigate about light intensity characteristic, promoting germination, and effect of cultivation to develop the raising of seedling and cultivation system for stable mass production of high value-ad
○ Development of seedling system and mass production system for Angelica gigas
This study was to investigate about light intensity characteristic, promoting germination, and effect of cultivation to develop the raising of seedling and cultivation system for stable mass production of high value-added medicinal Angelica gigas.
The results are as follows : the raising seedling system was consisted of the lighting device irradiating the led and fluorescent lamp independently and growing apparatus which it can bottom spray by the nozzle was designed and fabricated. The lighting device was made to vary about 10~40cm the investigation height. And growing apparatus with the cooler cooling the nutrient solution was made to can control the temperature of root zone.
When the light irradiation distance was 20cm, PPFD of LED raising seedling system was higher about 2.7~2.8 times PPFD of center area than the edge, PPFD of LED raising seedling system showed that the maximum was 338.4μmol m-2s-1, minimum was 123.5μmol m-2s-1, average was 246.3μmol m-2s-1, and PPFD of fluorescent lamp raising seedling system showed that the maximum was 112.7μmol m-2s-1, minimum was 40.2μmol m-2s-1, and average was 81.6μmol m-2s-1.
The result of investigation the germinating rate of the late fall Angelica gigas seed for 30 days by using the raising seedling system was improved 36.3%~43.0% in comparison with the conventional method. The germinating rate of it was increased 75.6% in treatment ultrasonic wave for 5 minutes, 74.4% in 10 minutes and 81.1% in 15 minutes.
The result of performing the cultivation experiment by using the LED, fluorescent lamp showed the good growth state at LED raising seedling system. Raising seedling system the number of leaf, leaf length, and leaf width except plant height were high in LED.
The result of the performance test of the Angelica gigas cultivation system was possible tray and pot cultivation and growth of the number of leaf, leaf length and leaf width was good.
○ Examination of optimum environmental conditions and development of a cropping system for Angelica gigas
The aim of this study was to investigate optimum growth conditions and a cropping system of Angelica gigas during experiments.
Angelica gigas ‘Manchu’ were sowed and managed under a growth room chamber.
The environmental conditions (temperature 22℃/18℃ (day/night), relative humidity 50-70%, photosynthetic photon flux density (PPFD) 120±6 μmol m-2 s-1) were maintained for 3 weeks. Forty eight seedlings with 4-5 leaves were transplanted in deep flow technique (DFT), substrate, and spray culture systems [culture bed: 800 (L) x 800 (W) x 400 mm(H)] under 150±5 μmol m-2 s-1 PPFD provided with fluorescence lamps and cultivated for 11 weeks. At the end of the experiment, fresh and dry weights, leaf lenghth and width, SPAD, root fresh, and dry weights, and root volume of Anglica gigas were measured. Photosynthetic rate of Anglica gigas were measured with portable photosynthesis systems to investigate optimum PPFD, CO2 concentration, and air temperature conditions.
Fresh and dry weights of Anglica gigas grown in substrate were significantly greater than DFT-treated, but there were not significant with spray treatment. At the condition of 22-23℃ nutrient solution temperature, fresh and dry weights of Anglica gigas were greater than the 25-26℃ temperature conditions. Leaf photosynthesis of Anglica gigas showed the tendency to sharply increase as PPFD was increased from 50 to 200 μmol m-2 s-1. Though CO2 saturation point was around 1000-1200 μmol mol-1, increase in air temperature from 16 to 26℃ did not quite affect photosynthesis of Anglica gigas. Fresh weight of Anglica gigas grown under the red, blue, white LED treatment during 3 weeks was higher than that of green LED treatment and decrusin contents of Anglica gigas grown under the yellow and green LED were highest among LED treatments after 2 weeks from the start of LED treatment. Soaking seeds of Anglica gigas at the Ca(NO3)2 0.3M for 3 days showed the higher germination rate among other treatments [K2HPO4 (0.1M, 0.3M, 0.5M), KNO3 (0.1M, 0.3M, 0.5M), Ca(NO3)2 (0.1M, 0.3M, 0.5M), DW].
Anglica gigas could be optimally cultivated with a spray culture system as air temperature, PPFD, and CO2 concentration for environment are controlled at 20±2℃, 150 μmol m-2 s-1, and around 1000 μmol mol-1, with red, blue, or white LED for mass production.
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