초록 ▼

Ⅳ. 연구개발결과
<1세부과제 : 국내 개발된 GM작물의 분자생물학적 평가를 통한 이벤트 선발>
○ 해충저항성 벼 Bt-9의 4계통 및 Bt-T의 4계통의 생물검정 및 도입유전자 발현분석을 수행하여 분석한 8계통 모두 혹명나방 저항성이 있음을 확인함.
○ Bt-9 및 Bt-T 8계통의 전주지역에서의 재배적 특성, 농업형질 및 환경적응성을 종합적으로 평가하여 Bt-9-30 및 Bt-T-07의 두 개 GM벼 이벤트를 선발하였다.

<1협동과제 : 국내개발 형질전환 벼·콩 이벤트에 대한 농업형질 및 생산성 평가

Ⅳ. 연구개발결과
<1세부과제 : 국내 개발된 GM작물의 분자생물학적 평가를 통한 이벤트 선발>
○ 해충저항성 벼 Bt-9의 4계통 및 Bt-T의 4계통의 생물검정 및 도입유전자 발현분석을 수행하여 분석한 8계통 모두 혹명나방 저항성이 있음을 확인함.
○ Bt-9 및 Bt-T 8계통의 전주지역에서의 재배적 특성, 농업형질 및 환경적응성을 종합적으로 평가하여 Bt-9-30 및 Bt-T-07의 두 개 GM벼 이벤트를 선발하였다.

<1협동과제 : 국내개발 형질전환 벼·콩 이벤트에 대한 농업형질 및 생산성 평가>
○ Bt-9 4계통의 수원지역에서의 재배적 특성, 농업형질 및 환경적응성을 종합적으로 평가하여 전주지역에서와 같이 Bt-9-30 이벤트를 선발하였다.
○ 형질전환 콩 PMD1(igf), PMD2(egf), PMD3(Trx) 계통의 131개 line을 비교평가 하여 최종적으로 6개 이벤트 line을 선발함.

<2협동과제 : GM감자의 이벤트화를 위한 농업특성 및 재료평가>
○ 다중 바이러스저항성 감자 600-3-1 및 600-3-2 계통의 재배적 특성과 농업형질을 평가하였으나, 모두 생육이 불량하고 수량성이 낮아 이벤트 부적격으로 판정함.
○ 노지 재배 상황에서 바이러스 감염 및 저항성 검정은 자연적 병 발생이 일어나자 않아서 분석 불가하였음.

<3협동과제 : 국내개발 가뭄저항성 벼 생리적 특성의 국외포장 검정>
○ 건조 스트레스 및 고농도 염 환경에서 가뭄저항성 벼(MsrB2)의 생리적 특성을 검정하여 대조구와 비교하였으나 정밀한 환경스트레스 조건하에서의 지속적인 분석이 필요하다는 결론을 도출함.

Abstract ▼

The purposes of this study was to select promising genetically modified (GM) events that are qualified for risk assessment and dossier documentation. In this study, several GM varieties dveloped by domestic scientists, including insect-resistant Bt rice, drought-resistant rice, multi-virus-resistant

The purposes of this study was to select promising genetically modified (GM) events that are qualified for risk assessment and dossier documentation. In this study, several GM varieties dveloped by domestic scientists, including insect-resistant Bt rice, drought-resistant rice, multi-virus-resistant potato, and nutrient enhanced soybean were tested and assessed over a one-year trial. The fundamental assessment criteria included 1) suitability for the intended purpose, 2) good agronomic traits in the field, and 3) adaptability to the agronomic environment. Based on the these criteria, 5 event lines from two transgenic crop plants were selected as candidates for risk assessment.
Among candidates from 8 Bt rice lines, Bt-9-30 with Ilmi-byo genetic background and Bt-T-07 with Dongjin-byo genetic background were selected. All 8 lines tested showed relatively strong resistance compared to their non-transgenic counterparts in a feeding experiment with rice leaf roller larvae. In the field test, Bt-9-30 and Bt-T-07 lines showed 106 % and 90 % yield, respectively compared to their counterparts. Overall, both Bt-9-30 and Bt-T-07 showed no difference in growth pattern, physiology, or adaptability to the agroecosystem.
Among 131 transgenic soybean lines from groups PMD1, PMD2, and PMD3 with egf, IGF, and Trx transgenes, respectively, 6 lines were selected as candidates for further field assessment. In field trials, the overall growth and yields were found to be poor compared to the average growth of soybean because of bad weather conditions during the trial periods. Despite these conditions, compared to the control and references, 6 candidate lines from the 131 were selected.
Unfortunately, no candidates were selected from the multi-virus resistance potato plants (600-3-1 and 600-3-2) harboring anti-sense gene constructs of the partial coat protein sequences in PVY, PVA, and PLRV. Preliminary results from transgenic potato infection experiments revealed reasonable resistance to infection with multiple viruses, however, overall growth and yield were poor compared to the control. The total number of tubes according to fresh weight was lower in GM potato lines than in non-GM potato lines. Viral resistance of non-GM and GM potato lines after cultivation in net-houses and open field trials were also determined by visual inspection and ELISA; no virus infected potato plants were observed, regardless of genetic background.
In summary, we analyzed 131 lines from transgenic rice, potato, and soybean to select 8 candidate event lines based on agronomic traits and adaptability to the agricultural environment. in light of these findings, the selected candidates lines will be examined for safety and risk assessment documentation for the petition of these GM cultivarss.