Abstract ▼

Because we don’t have improved chicken pure line, commercial chicken farmers should import grand parent stock(GPS) every year. Due to this, we should pay plenty of money to import GPS. The purpose of GSP chicken project is to develop new breed using indigenous breed. At this project“purification of

Because we don’t have improved chicken pure line, commercial chicken farmers should import grand parent stock(GPS) every year. Due to this, we should pay plenty of money to import GPS. The purpose of GSP chicken project is to develop new breed using indigenous breed. At this project“purification of indigenous chicken breed for producing new breed and establishment of breeding strategy”, 12 strains from National Institute of Animal Science and 12 strains from Hanhyup company are used. This project might 3 categories as follows:
1) Purification pure line through theological selection
2) Stabilized genetic resources conservation
3) Establishing D/B and manage ths data acquired from GSP chicken project

From 12 strains which Hanhyup company, total of 19,555chicks(♀12,262수 ♂7,293) were produced and body weight, shank length, egg production ability were recorded individually.
Whit those data, second generation were also produced(♀8,329, ♂4,137 total 12,466) They also recorded economic traits individually.

The viability of frozen/thawed chicken semen was examined for the success of chicken semen freezing. When the semen was frozen using 9% MA, the fertilization rate was about 35% and the hatching rate was about 90% with HS-1 diluent. The differences between fertilization rate and hatching rate were still lower than those of fresh semen. To freeze chickery semen properly, MA cryoprotectant is usually used at a concentration of 7.5 to 9%. With higher concentration of MA upon 10%, the more dilution shock of chicken semen was shown with higher survival rate after freezing. Immediately after thawing, abnormality of sperm was examined by using Diff-Quik staining methods. When thawed sperm was cultured at 32 ℃ for 5, 30 and 60 minutes after thawing at 5 ℃ for 2 min, the ratio of normal acrosome was 55.6%, but decreased to 35.5%, 26.3% and 28.6%. The method of using CLC in chicken semen did not significantly increase the survival rate of frozen chicken semen. With increasing the cholesterol, the survival rate after thawing was shown as 78.3% and 79.3% at 1 mg/ml and 3 mg/ml treatment but control was 84.9%. Also the antioxidant of NAC was adjusted to 1 mM, 2.5 mM and 5 mM, the survival rate was increased at 2.5 mM and 5 mM without significant differences. When Rhodamine 123 was used to measure of mitochondrial activity, there are no significant difference between 1 mM and 5 mM NAC treated semen. In order to analyze the stability of the chromosomes of the frozen chicken sperm, the slides were stained with Diff-Quik reagent. In the fresh sperm, the sperm viability was 93.53%, 82.42% and 90.63%, respectively, and the steady state of Diff-Quik chromatin was 87.96%, 74.25% and 85.10%, respectively. When the same samples were frozen and thawed, the survival rate of the semen was 69.58%, 61.98% and 72.20%, respectively. The chromatinity was 58.91%, 48.49% and 63.34%, respectively. These results shows that the quality of chicken semen can be judged by the presence of chromatin stability of spermatozoa that were stained normal with Diff-Quik staining method. Especially, this staining method suggested useful and simple judging tool of good quality frozen chicken semen by microscopic observation of the semen smear slides. To increase the survival rate of frozen chicken semen, the ratio of mixed cryprotectant with MA and DMF was examined with survived spermatozoa. When the 4:3 ratio of final diluent was used, the survival rate was higher than 2:5, 3:4 or 5:2 mixed ratio of final diluents. It is expected that these mixture diluent with MA and DMF will be possible to provide a freezing method necessary for the later cryopreservation of genetic resources.
Even though many researchers have reported fertility of frozen chicken semen, the data on fertility and hatching ability are still differed with higher deviations.

To establish the efficient collection, accumulation and management of chicken breeding information, GSP Chicken Bioinformatics System(GSP-CBIS) has been developed. To serve this system on internet, the domain name ‘gsp-cbis.kr’ and ‘gspcbis.kr’ was secured and activated. This database system includes individual, breed line, population, pedigree,phenotype and genotype information. The database system was designed as relation-oriented DB, and user-friendly in/output interface was implemented. For given research period, out of 24 pure breeds, 53 mating combination and 77 breed lines, total 221,092 chicken information has been stored and a lot of phenotype and genotype data has accumulated. To utilize this information for research and industry, tools for basic statistical analysis was developed and implemented to this system.

Introducing new breed need plenty of time and data. This project is such as base camp of GSP chicken project. Thus data will be continuously collected at second phase project and support other project. And developed analysis tools are bing used by researchers and industry officials among other project participants. In terms of animal genetics and bioinformatics, using this system, information will be shared and utilized for more detailed and professional purpose. The frozen semen is the basic technology of breeding system and reproduction in husbandry animals. However, the utility of frozen chickery semen is constructed for laboratory basis. To intensify basic data and genetic resources production technique, the more data of frozen semen should be studied in this research area.

( 출처 : SUMMARY 6p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 6
• CONTENTS ... 8
• 목차 ... 9
• 제 1 장 프로젝트의 개요 및 성과목표 ... 10
• 제 1 절 연구개발의 목적 ... 10
• 제 2 절 연구개발의 필요성 ... 10
• 제 3 절 연구성과 목표 및 실적 ... 13
• 제 2장 국내외 기술개발 현황 ... 14
• 제 1절 국내 토종닭 사육현황 ... 14
• 제 2절 외국의 토종닭 사육현황 ... 16
• 제 3 절 유전자원의 보존방안 ... 18
• 제 4 절 생물정보 D/B 활용 육종전략 수립 현황 ... 20
• 제 3 장 연구개발 수행 내용 및 결과 ... 21
• 제 1 절 연구개발 수행 내용 ... 21
• 제 2 절 국립축산과학원 보유 계통 연구 ... 26
• 제 3 절 ㈜한협 보유 계통 연구 ... 36
• 제 4 절 닭 정액 동결보존 방법의 개선 ... 66
• 제 5절 수집집단의 생물정보 database를 활용한 육종전략 수립(제2세부프로젝트) ... 82
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 117
• 제 1 절 연도별 세부연구목표 달성도 ... 117
• 제 2 절 관련분야 기술발전 기여도 ... 121
• 제 5 장 연구개발 성과 및 성과활용계획 ... 122
• 제 1 절 연구 성과 ... 122
• 제 2 절 활용계획 ... 125
• 제 6 장 연구개발 과정에서 수집한 해외과학기술정보 ... 126
• 제 7 장 참고문헌 ... 128
• 첨부. 특허, 논문, 제품(시장) 분석보고서 ... 133
• 끝페이지 ... 139