초록 ▼

< 인삼 종자 채종 효율성 향상 연구 >
○ 인삼의 연생 및 품종별 종자 생산량은 3, 4, 5, 6년생에서 각기 13.9∼32.2, 49.3∼77.6, 55.2∼86.8, 76.3∼106.5kg/10a로 나타남

○ 묘삼무게의 증가에 따라 2년생에서 개화개체 비율 및 개화량이 증가하였으며, 3년생에서도 개화량이 증가함
- 인삼종자를 조기에 대량생산하기 위해서는 1.0g 이상의 대묘를 사용하는 것이 유리함

○ 무채종에 비해 1, 2, 3, 4회 채종 시 지하부 인삼의 수량은 각기 21.3~27.5%

< 인삼 종자 채종 효율성 향상 연구 >
○ 인삼의 연생 및 품종별 종자 생산량은 3, 4, 5, 6년생에서 각기 13.9∼32.2, 49.3∼77.6, 55.2∼86.8, 76.3∼106.5kg/10a로 나타남

○ 묘삼무게의 증가에 따라 2년생에서 개화개체 비율 및 개화량이 증가하였으며, 3년생에서도 개화량이 증가함
- 인삼종자를 조기에 대량생산하기 위해서는 1.0g 이상의 대묘를 사용하는 것이 유리함

○ 무채종에 비해 1, 2, 3, 4회 채종 시 지하부 인삼의 수량은 각기 21.3~27.5%, 34.6~38.4, 43.4~45.3, 55.1~57.4 감소

○ 일반적으로 묘삼 및 개갑종자의 휴면타파를 위해선 90일 정도의 저온처리가 필요한데, GA₃와 변온 동시처리를 통해 휴면을 조기에 타파할 수 있었음
- 묘삼의 경우 30일 처리에서 안정적 출현(90%)이 가능하며, 종자는 30일에서 67.4%의 출현율을 나타냄

< 인삼 기내 대량증식기술 개발 연구 >
○ 인삼 선발라인의 기내배양을 통하여 체세포배를 대량으로 유도하였으며, 유도된 체세포배는 휴면이 있음을 확인
○ 체세포배의 휴면타파를 위해서 저온 또는 GA₃ 처리가 필요
○ 저온처리 후 발아된 체세포배로부터 얻어진 식물체는 뿌리의 비대가 진행됨
○ 기내 배양으로부터 비대된 뿌리를 토양에 옮긴 후 저온 또는 GA₃ 처리 후 새로운 지상부 잎이 출현되어 식물체 토양순화에 성공함

(출처 : 보고서 요약서 p.3)

Abstract ▼

< Improvement of seed production effectiveness in Panax ginseng >
○ Characteristics of flowering and fructification of major varieties
- The emergence and flowering date of Chungsun was the earliest among the ginseng varieties. Sunhyang showed the opposite result for the emergence and flowerin

< Improvement of seed production effectiveness in Panax ginseng >
○ Characteristics of flowering and fructification of major varieties
- The emergence and flowering date of Chungsun was the earliest among the ginseng varieties. Sunhyang showed the opposite result for the emergence and flowering date. The emergence date of Chunpoong was not late compared with the other varieties, but the flowering date was late.
- Seed production of ginseng was 13.9~32.2, 49.3~77.6, 55.2~86.8 and 76.3~ 106.5kg/10a, respectively, in three, four, five and six-year-old varieties.

○ Flowering and growth characteristics by seedling weight and culture method
- In 2yr ginseng, the ratio of flowering plants and number of flower incresed in proportion to the weight of seedling, and in 3yr ginseng, number of flower increased.
- In 2~3yr ginseng, the growth characteristics of heavy seedlings was in good condition, and in 3yr ginseng, root yield increased in proportion to the weight of seedling within range from below 0.6g to 1.2g.
- Seed production of 3yr ginseng was 15.2, 21.0, 23.3, 21.1, 24.5 and 29.5kg/10a, respectively, in 7×9, 7×11, 7×13, 9×9, 9×11 and 9×13.
- Flower formation of ginseng was completed in August of last year.

○ Growth, yield and component characteristics of ginseng by number of seed harvesting times
- Ginseng root production decreased 21.3~27.5, 34.6~38.4, 43.4~45.3 and 55.1~57.4%, respectively, in one, two, three and four times seed production compared with no seed-gathering.
- When the seed harvesting times were less than 2 times for six years, the saponin content in white ginseng of Yunpoong and Gumpoong was 0.48% and 0.08%, respectively, lower than that of three or more times but, was not significant.

○ Dormancy period shortening of seed and seedling, and improvement of stratification rate
- The rate of bud emergence for Chungsun was 32∼92% in 15∼60 days after the alternating temperature(AT) and GA₃ treatment.
The emergence rate of Sunun was 60∼98%. Normal growth of the ginseng seedling was observed in spite of early breaking of bud dormancy by the combined GA₃ and AT treatments.
- Germination characteristics of ginseng seeds was improved by GA₃ treatment especially combined with alternating temperature.
- GA₃ and AT treatments shortened the dormancy period and facilitated the stable emergence of ginseng seed and seedling.
- Stratification rate of major varieties was 34.1 and 78.0%, respectively, in 50 and 60days seed-gathering after flowering.

< Development of In vitro propagation of Panax ginseng >
○ Mass propagation of plantlets via somatic embryogenesis of selected ginseng lines
- There were difference in the rate of somatic embryo induction among 9 different lines. The three lines (5, 7, 9) showed more than 50% of somatic embryogenesis induction rate.
- MS medium among 6 different media was the bast for somatic embryo induction.
- Induction of germination of somatic embryos was required the treatment of more than 10 mg/l GA₃.

○ Improvement of mass propagation of plantlets via somatic embryogenesis
- In vitro regenerated ginseng plantlets showed root thickening during culture.
- Root thickening was stimulated during bioreactor culture as the concentration of sucrose in medium was increased.
- There are positive relationship between root thickening and senescence of leaves.

○ Development of soil transfer technology of in vitro propagated ginseng plants
- Thickened roots with leaf senescence showed the tendency of dormancy.
- Shoots sprouting occurred after cold and GA₃ treatments of thickened roots.
- Soil transfer of thickened roots does not require special soil acclimatization process.
- Through above technology of in vitro ginseng propagation, interspecific hybrid plants between Korean and American ginseng propagated successfuly.

(출처 : Summary p.6)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 6
• 목차 ... 8
• 제 1 장 연구개발 과제의 개요 ... 9
• 제 2 장 국내외 기술개발 현황 ... 9
• 제 3 장 연구 수행 내용 및 결과 ... 11
• 제 4 장 목표달성도 및 관련분야 기여도 ... 81
• 제1절 : 목표대비 달성도 ... 81
• 제2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 81
• 제 5 장 연구 결과의 활용 계획 ... 82
• 제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 82
• 제 7 장 연구 개발 결과의 보안 등급 ... 82
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 82
• 제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 82
• 제 10 장 연구개발과제의 대표적 연구실적 ... 83
• 제 11장 기타사항 ... 83
• 제 12 장 참고문헌 ... 84
• 끝페이지 ... 89