Abstract ▼

1. Investigation and development of microalgae and seed bank operation
○ Culture collected 12 strains from domestic
- Taxonomically 4 strains of chlorophyceae, 6 strains of bacillariophyceae and 2 strains of haptophyceae

○ Culture collection from domestic and foreign microalgae and seed

1. Investigation and development of microalgae and seed bank operation
○ Culture collected 12 strains from domestic
- Taxonomically 4 strains of chlorophyceae, 6 strains of bacillariophyceae and 2 strains of haptophyceae

○ Culture collection from domestic and foreign microalgae and seed bank operation
- Total culture collected microalgae were 46 species which were haptophyceae 13 strains (cf. Isochrysis galbana), chlorophyceae 7 strains (cf. Nannochloris oculata) and 19 strains of bacillariophyceae (cf. Chaetoceros gracilis).
- Results of seed bank operation total 5 species were distributed 339 times to hatchery fisherman etc.
- Development 3 species; Issochrysis sp, Phaeodactylum sp. Tetrselmis sp., which were developed for the seasonal strains (summer type)

○ Development various culture system for the microalgae culture
- Stock culture system, high density mass culture system applied with plant growth light emitting diode, LED (Korea patent # 10-1270648), intermediated culture system was developed during studies.

2. Study of optimum culture conditions
○ Development of efficiency and low costs culture mediums
- Developed 5 organic N source culture mediums using agricultural fertilizers
- Appling organic N source culture mediums at I. galbana for mess culture experiment were shown higher growth rate than Conway medium. Cell density was reached 547 x 104 cells/mL and then declined after 7 days cultured with Conway medium but culture with SEI-2, SEI-4 and SEI-5 organic medium was grown 813 x 104 cells/mL, 783 x 104 cells/mL and 875 x 104 cells/mL after 14 days, respectively.
- Developed 4 modified culture mediums based on Conway medium in which modified culture medium was suitable using in domestic shellfish hatcheries.

Tried to culture experiments with self-modified culture mediums at I. galbana, T. tetrathele, P. triconutum, C. gracilis results were shown very effectiveness.
- Establishment of optimum cultural environment conditions as water temperature and salinity effects at the 4 microaglae species (I. galbana, T. tetrathele, P. triconutum, C. gracilis), the most growth rate was shown as this; I. galbana was 24.0℃, 24.0 psu, T. tetrathelewas 27.0℃, 36.0 psu, P. triconutum was 16.0℃, 30.0 psu and C. gracilis was 24.0℃, 30.0 psu
- Evaluation of nitrogen and phosphorus nutrient consumption by I. galbana, Tetraselmis sp. and Phaeodactylum sp. during culture periods. All most of N. P nutrients were exhausted after 6 days cultured with self-modified culture medium at 22.0℃.
- Assessment of optimum N/P ratio in the culture medium. Using two species (I. galbana, Tetraselmis sp.) of microagle ware tested. N/P ratio in culture medium was appropriated at 8:1.
- Developed ultra-fine filteration and sterilization system for cultural sea water treatment. This system was very good effectiveness for treatment of the cultural sea water which can be appling for the mass microalgaqe culture at the hatcheries.

3. Technique development of enhancement microalgae utilization in hatchery.
○ Enhancement technique development of mcroalgae utilization with nutrient composition and cell size.
- Ingestion rate of I. galban, I. aff. galbana, P. lutheri, C. ellipsoidea, N. oculata were 94.2-99.7% all size of oyster larvae but C. calcitrans, C. gracilis, C. simplex could be ingested 90.0% over shell length reach to 189.3±13.8 μm.
- P. triconutum, D. tertiolecta, T. tetrathele were not ingested under shell length 65.0-100.0 μm of D shaped larvae. but after this was ingested 97.3-99.7%, 43.3-99.3%, 48.5-99.3%, respectively. However T. weissflogii could not be ingested all stage of larvae.
- Microalgae culture with four culture mediums (self modified 2, Conway and F/2) feeding test was conducted for Manila clam larvae. Results was not significant difference.
- The nutrient compositions (crude protein, lipid, carbohydrate, fatty acids and amino acids) were very different in the microaglae cells by the culture mediums.

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 보고서 요약 ... 2
• 요약문 ... 3
• SUMMARY ... 7
• 목차 ... 9
• CONTENTS ... 11
• 그림목차 ... 14
• 표목차 ... 15
• FIGURE LISTS ... 16
• TABLE LISTS ... 18
• 제 1 장 서론 ... 19
• 제 2 장 국내·외 기술개발 현황 ... 21
• 가. 국내 기술개발 현황 ... 21
• 나. 국외 기술개발현황 ... 21
• 제 3 장 연구개발 수행 내용 및 결과 ... 22
• 가. 미세조류 개발탐색 및 Seed bank 운영 ... 22
• 1) 각론 ... 22
• 2) 재료 및 방법 ... 22
• 3) 결과 및 고찰 ... 23
• 나. 최적 대량 배양조건 연구 ... 33
• 1) 각론 ... 33
• 2) 재료 및 방법 ... 35
• 3) 결과 및 고찰 ... 38
• 다. 고밀도 대량배양 시스템 개발 ... 48
• 1) 각론 ... 48
• 2) 재료 및 방법 ... 50
• 3) 결과 및 고찰 ... 51
• 라. 미세조류 이용성 제고방안 요소 기술 개발 ... 53
• 1) 각론 ... 53
• 2) 재료 및 방법 ... 55
• 3) 결과 및 고찰 ... 59
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 67
• 제 5 장 연구개발결과의 활용계획 ... 68
• 제 6 장 참고문헌 ... 69
• 끝페이지 ... 80