초록 ▼

∘주요 수산 양식생물의 육종기술 및 육종 신품종 개발 추진
- 대상품종 : 넙치, 전복(2004년∼), 멍게(2009년∼), 돌돔(2011년∼)
- 육종방법 : 유전자 표지를 이용한 분자육종 및 전통적인 선발육종
- 대상형질 : 넙치(성장, 체형, 질병내성), 전복(성장, 수온환경내성), 멍게, 돌돔(질병내성)
∘육종형질의 지속적인 개선 및 육종집단의 유전다양성 유지를 위한 핵집단 가계관리
- 육종넙치 4세대 291가계, 육종전복 3세대 235가계
∘육종형질 개선
- 육종넙치 유전력 : 성장형질

∘주요 수산 양식생물의 육종기술 및 육종 신품종 개발 추진
- 대상품종 : 넙치, 전복(2004년∼), 멍게(2009년∼), 돌돔(2011년∼)
- 육종방법 : 유전자 표지를 이용한 분자육종 및 전통적인 선발육종
- 대상형질 : 넙치(성장, 체형, 질병내성), 전복(성장, 수온환경내성), 멍게, 돌돔(질병내성)
∘육종형질의 지속적인 개선 및 육종집단의 유전다양성 유지를 위한 핵집단 가계관리
- 육종넙치 4세대 291가계, 육종전복 3세대 235가계
∘육종형질 개선
- 육종넙치 유전력 : 성장형질(1세대 0.755, 2세대 0.421, 3세대 0.428, 4세대 0.300), 체형형질(1세대 0.452, 2세대 0.414, 3세대 0.414)
- 육종넙치 질병내성 : 에드워드 2.0%, VHSV 0.3%, 스쿠티카 1.9% 내성 개선
- 육종전복 유전력 : 각장(1세대 0.50, 2세대 0.307, 3세대 0.387), 각폭(1세대 0.49, 2세대 0.290, 3세대 0.382), 중량(1세대 0.510, 2세대 0.411, 3세대 0.381), 고수온(0.214), 저수온(0.279)

∘넙치 질병내성 연관유전자 지도 : 242개 유전자마커, 23개 그룹의 연관유전자 지도

∘돌돔 및 멍게 육종기반기술 개발
- 유전자 표지 개발(돌돔 175개, 멍게 35개), 친자확인기술 개발(돌돔 16개, 멍게 7개)
- GS-FLX 염기서열 분석을 통해 돌돔 내병성관련 유전자 구조 및 특성 분석 : 45개
- 멍게 양성현장 시험을 통한 선발육종 효율성은 낮은 것으로 판단

∘육종품종 산업화 추진
- 육종넙치 산업화 : 2010년 조기 산업화 추진 및 킹넙치 브랜드 개발, 해외시장 개척
- 육종전복 산업화 : 2013년 현장 양성효율 검증을 통해 2015년 산업화 추진

(출처 : 보고서 요약 2p)

Abstract ▼

Ⅳ. Result of research and development
The development of breeding techniques and new breeding species lead to improvement of productivity and promotion of aquaculture industry with the international competitiveness. We carried out the study of this assignment followed since 2004.

1. Develo

Ⅳ. Result of research and development
The development of breeding techniques and new breeding species lead to improvement of productivity and promotion of aquaculture industry with the international competitiveness. We carried out the study of this assignment followed since 2004.

1. Development of selective breeding technology for genetically improved flounder In 2004, genetically diverse flounder populations were collected from East, West, South Sea population and cultured and wild population, and produced genetically improved flounder (F1), consisting of 245 family, using breeding guidelines based on the genetic relationship. The nuclear groups were bred under the same breeding condition to 11 months and carried out PIT-Tag insertion for individual identification, measurement of trait, household analysis by paternity, evalution of genetic ability. The growth-related traits periodically confirmed to improve growth traits and evaluated the genetic variation and ability between households through parasites, bacteria and virus artificial infection experiments to improved disease resistance.

Breeder candidates were selected for the next generation production based paternity test and growth, body shape, and artificial infection experiments. The second generation of 239 households, the third generation of 279, the fourth generation of 291 were produced using genetic relationship and kinship information between selected breeder candidates. Genetically improved flounder heritability of growth traits (body weight) was estimated first generation 0.755, second generation 0.421, 0.428 3rd generation, 0.300 4th generation. When selection weights index given as 10 percent based on the results of artificial infection experiment, the weight of the F4 is 16.9%, Edward resistance is 2.0%, VHSV 0.3% tolerance, Scutica resistance improved by 1.9% than F3, these result suggest that improved disease resistance varieties were expected to continue to develop.

SNP analysis confirmed a total of 1,098 SNPs though single nucleotide mutations of immune disease resistance-related genes in genetically improved flounder and gene linkage maps were prepared with artificially infected experiment with VHS virus and genotype of flounder family using 242 marker.

2. Development of breeding techniques of genetically improved abalone
Wild and cultured breeder abalones were collected from East, West and South Sea to ensure genetic diversity and obtained 231 microsatellite DNA for analysis of paternity and genetic diversity. Breeding guidelines were established considering the genetic relationships of breeder candidates and the first generation was produced 204 households.

Breeding guidelines were prepared and the second generation of 172 households, the third generation of 251 were produced using genetic relationship and kinship information between selected breeder candidates.

The nuclear groups were bred under the same breeding condition after fertilization and carried out PIT-Tag insertion for individual identification, periodic measurement of trait, household analysis by paternity, evalution of genetic ability.

Genetically improved abalone exposure under artificially temperature to development of abnormal water temperature resistance varieties, which heritability of low and high water temperature is 0.279 and 0.214, respectively and possibility of genetic improvement was confirmed.
When further selection of breeder candidate, giving weight to selection of growth and high and low water temperature resistant trait that the result is expected to improvement of breeding trait.

3. Development of breeding technique of genetically improved rock sea-bream
Basis breeder groups were collected for development of genetically improved rock sea-bream, F1 households were successfully produced by over the 4th of spawning and artificial fertilization.
Genetic diversity and relationship between the rock sea-bream breeder group were analyzed using genetic marker and prepared the combination of multiplex PCR configuration and technology for paternity.

Appropriate 175 rock sea-bream microsatellite markers for genetic diversity analysis and paternity were developed and analyzed the genetic diversity and genetic relationship between maternal group and then selected 16 MS markers derived singleton.

The gene structure and biochemical and molecular biological characteristics was analyzed using GS-FLX and 45 genes were analyzed for the genomic DNA structure among the BAC library of 92,160 clones by researches commissioned. Artificial infection experiments of Irido virus show that expression of disease resistance-related genes such as perporin, granzyme, Fas, Fas lagand, caspas and IAP was significantly varied depending on the virus treatment. Also, we found the improtance of water temperature that die and recovery of Irido virus infected rock sea-bream and established the development of virus-resistant varieties based technologies.

4. Development of breeding technique of genetically improved sea squirt
Sea squirt is a major hatchery species Gyeongnam and Gyeongbuk, but mortality rate of 65-95% and high amount of damage caused more than 20 billion won per year due to softness syndrome, therefore attribution and preparation is required.

A total 9004 of nine groups sea squirts were obtained to secure the breeder from Korea and Japan wild and farmed for production of genetically improved sea squirt. Environmental control, such as photoperiod and water temperature was confirmed to inducing the maturation of the breeder squirts. As a result, gonadal weight index increased in each test phrases but there was no significant difference.

The genetically improved sea squirts that maximized the genetic diversity of populations was cross-breeding between squirts breeder groups and carried out production, seedling collection, and cultivation test. The dispersion cultivation experiment of sea squirt seed showed that the death by softness syndrome was exhibited a variety of different 0.5% to 60%.

The genetic markers of sea squirts were developed through the gene expression search and genetic diversity and genetic relationship were analyzed using seven of the total of 35 markers. As a result, the genetic diversity of Korea's wild sea squirt is slightly higher than in Japan and cultured sea squirt lower than wild in every respect. Genetic diversity and relationship between the sea squirt breeder groups were analyzed using genetic marker and prepared the combination of multiplex PCR configuration and technology for paternity.

Survival rate of cultivated sea squirt very low due to influence of softness syndrome, hurricanes and high water temperature, therefore breeding efficiency is low though evaluation of heritability and securing of mature breeder candidates.

5. Industrialization of genetically improved varieties (flounder, abalone)
Since 2004, the genetically improved flounder and abalone were developed and promoted industrialization based technology such as field application and verification of effectiveness. The commercialzed line with excellent growth and body type was selected and first produced in 2007, also the genetically improved flounder with rapid growth and disease resistance was produced in 2009. The research of breeding efficiency shows that growth traits of commercialzed line is significantly higher compared to the normal control group. The field application of commercialzed line was planned in 2013, but three years ahead of schedule so that the fertilized egg to spread across the country to crisis of the aquaculture industry and depending on the needs of fishermen.
The peculiar brand (King flounder) was developed in order to give identity and differentiation of genetically improved flounder. The domestic and international trademark registrations were completed in the main consumer markets such as USA, Japan, China, and Europe in order to prevent plagiarism.

The genetic improved flounder was exported to the U.S for the first time using live fish transport container in 2011, also successfully produced by more than 1kg in the USA field. Because fast-growing of the King flounder was proved in USA field, flounder distribution center was built in Jeju and constructed efficient supply system in response to the expanding needs of supply.

(출처 : SUMMARY 9p)

목차 Contents

• 표지 ... 1
• 보고서 요약 ... 2
• 요약문 ... 3
• SUMMARY ... 9
• 목차 ... 14
• CONTENTS ... 16
• 그림목차 ... 18
• FIGURE LISTS ... 20
• 표목차 ... 23
• TABLE LISTS ... 25
• 제 1 장 연구개발 과제의 개요 ... 28
• 제 1 절 연구개발 배경 ... 28
• 제 2 절 연구개발 필요성 ... 29
• 제 3 절 연구개발 목표 ... 30
• 제 2 장 국내외 기술개발 현황 ... 32
• 제 3 장 연구개발 수행 내용 및 결과 ... 34
• 제 1 절 넙치 육종기술 개발 ... 34
• 1. 재료 및 방법 ... 34
• 2. 결과 및 고찰 ... 36
• 3. 요약 ... 49
• 제 2 절 전복 육종기술개발 ... 50
• 1. 재료 및 방법 ... 50
• 2. 결과 및 고찰 ... 52
• 3. 요약 ... 62
• 제 3 절 돌돔 육종기술개발 ... 63
• 1. 재료 및 방법 ... 63
• 2. 결과 및 고찰 ... 64
• 3. 요약 ... 72
• 제 4 절 멍게 육종기술개발 ... 73
• 1. 재료 및 방법 ... 73
• 2. 결과 및 고찰 ... 75
• 3. 요약 ... 90
• 제 5 절 육종품종 산업화 추진 연구 ... 92
• 1. 재료 및 방법 ... 92
• 2. 결과 및 고찰 ... 95
• 3. 요약 ... 108
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 110
• 제 5 장 연구개발 결과의 활용계획 ... 111
• 제 6 장 참고문헌 ... 112
• 끝페이지 ... 113