초록

1. 연골판 결손 치료용 주입식 생리활성 하이드로겔 개발
① 슬관절 반월상 연골판 결손 동물 모델 확립
② 개발된 생리활성 하이드로겔을 최소 침습적인 방식으로 주입식 투여할 수 있는 적용 기술 개발
③ 연골판 결손 재생 치료용 차세대 의료기기로의 개발 가능성 확인
④ FDA 승인된 생체재료 적용
⑤ 향후 임상시험 진행 가능
⑥ 연골판 결손 재생 치료용 주입식 의료기기로서 제품화 가능성 제시
⑦ 광중합형 하이드로겔 시스템 개발

(출처 : 보고서 요약서 3p)

Abstract ▼

Purpose and content
The ultimate goal of this project is to develop injectable bioactive hydrogel for treatment of knee meniscus defect. In execution of this project, we selected candidate biomaterials suitable for this application, confirmed their in vivo potential to induce meniscus regeneratio

Purpose and content
The ultimate goal of this project is to develop injectable bioactive hydrogel for treatment of knee meniscus defect. In execution of this project, we selected candidate biomaterials suitable for this application, confirmed their in vivo potential to induce meniscus regeneration, and explored different technologies to optimize treatment effects.
Part 1 (1) established an animal model for knee meniscus defect, (2) devised an injection scheme for application of bioactive hydrogel to animal models, (3) confirmed meniscus regeneration induced by bioactive hydrogel in in vivo model and (4) additionally incorporated growth factors to bioactive hydrogel to further enhance meniscus regeneration. Part 2 (1) developed bioactive hydrogel that is injectable via in situ photopolymerization, (2) optimized hydrogel activity to promote cell migration from surrounding native tissue to defect site and enhance tissue regeneration, and (3) developed platelet-rich plasma (PRP)-containing adhesive bioactive hydrogel that is injectable via in situ polymerization.

Results
○ This project revealed the optimal composition of FDA-approved biomaterials to fabricate regenerative hydrogel composite for menicus tissue regeneration.
○ Injection method was developed as a minimally invasive way of delivering the bioactive hydrogels to the lesion site.
○ Animal model of meniscus defect has been well established.
○ Regenerative hydrogel composite was non-surgically, minimally-invasively delivered to the meniscus defect site via injection with syringe.
○ Through evaluation of tissue regeneration in animal model, regenerative hydrogel composite has shown great potential as next-generation medical device for the regenerative treatment of meniscus defect.
○ Since only FDA-approved biomaterials were employed in this study, clinical application seems highly possible. Therefore, after clinical trials, this injectable regenerative hydrogel composite can be developed into a commercialized meniscus regeneration solution.
○ Tissue-adhesive biopolymer was synthesized and enabled to in situ polymerize to fabricate injectable hydrogel system
○ FDA-approved substance (riboflavin; vitamin B) was employed as photoinitiator to produce photopolymerizeable hydrogel system, whose efficacy has been demonstrated in vivo .
○ Bioactive hydrogels developed in this study were applied to study the effect of cell-cell communication and RGD peptide on adult stem cell differentiation into meniscus tissue.

Expected Contribution
1. Regenerative hydrogel composite has shown great potential as next-generation medical device for the regenerative treatment of meniscus defect, a common knee injury which currently does not have a viable solution other than allograft implantation.
2. Torn meniscus can cause pain and swelling, impair knee function and predispose the joint to degenerative osteoarthritis. By enabling the regeneration of meniscus defect, patients can enjoy active lifestyle as prior to injury and decrease incidence of degenerative osteoarthritis.
3. The end-product of this research can be easily injected into defects, regardless of shape or size of defect. Therefore, this treatment can be applied to a lot of patients who need meniscus regeneration.
4. Results obtained from this research will help understand optimum conditions for meniscus regeneration and develop effective and more advanced biomaterial-based medical device.
5. In the future, progress into SIT and commercialization seems to be highly possible, leading to the improvement in national welfare and promotion of national competitiveness.

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 5
• SUMMARY ... 7
• CONTENTS ... 9
• 목차 ... 10
• 1. 연구개발과제의 개요 ... 11
• 1-1. 연구개발 목적 ... 11
• 1-2. 연구개발의 필요성 ... 11
• 1-3. 연구개발 범위 ... 13
• 2. 국내외 기술개발 현황 ... 16
• 3. 연구수행 내용 및 결과 ... 17
• 4. 목표달성도 및 관련분야 기여도 ... 87
• 4-1. 목표달성도 ... 87
• 4-2. 관련분야 기여도 ... 89
• 5. 연구결과의 활용계획 ... 90
• 6. 연구과정에서 수집한 해외과학기술정보 ... 92
• 7. 연구개발성과의 보안등급 ... 93
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 94
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 95
• 10. 연구개발과제의 대표적 연구실적 ... 96
• 11. 기타사항 ... 97
• 12. 참고문헌 ... 98
• 별첨 ... 100
• 끝페이지 ... 141