[보고서]근권 미생물시스템 활용 식물성장 개선 연구실

이귀재

보고서 정보

주관연구기관

전북대학교
Chonbuk National University

연구책임자

이귀재

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2016-11

과제시작연도

2015

주관부처

미래창조과학부
Ministry of Science, ICT and Future Planning

등록번호

TRKO201800006368

과제고유번호

1711028122

사업명

기초연구실지원

DB 구축일자

2018-05-12

키워드

염해.중금속.근권 미생물.형질전환.식물생장촉진.유전체.생물자원.생물학적 펌프.미생물 시스템.생물유전소자.salt stress.Heavy metal.rhizobacteria.transgenic plant.plant growth promotion.genomics.bioresources.

표 Neighbor-Joining Bootstrap methods in CLC Main Workbench (5.2) software, illustrating phylogenetic relationship of Herbaspirillum sp. GW103 based on the deduced amino acid sequences of acdS gene. Bootstrap values are from 100 bootstrap repetitions.
표 Growth of isolate in DF minimal medium under different of ACC concentration (A) and ACC deaminase activity from Herbaspirillum sp. GW103 strains (B). Each value is the mean of triplicates.
표 Root and shoot biomass of Chinese cabbage to 100mM NaCl level
표 Shoot ions content and ratio of chinese cabbage after 4 weeks exposure to 100 mM NaCl level
표 Root ions content and ratio of chinese cabbage after 4 weeks exposure to 100 mM NaCl level
표 Whole genome sequence method.
표 The genome of Herbaspirillum sp. GW103. From inside to outside 1) G+C content; 2)GC skew; 3) Genes color-coded according the COG functional categories.
표 Functional categories of the genome of Herbaspirillum sp. GW103 based on COG
표 A pairwise comparison of the Herbaspirillum seropedicae SmR1 and the the Herbaspirillum
표 The identification of gene clusters. A: Hemolysins, B: Cellulose synthase, C: Cellulase, D: Hemagglutins, E: Auxin biosynthesis, F: ACC deaminase, G: Tryptophan biosynthesis.
표 Result from next-generation sequencing of Bacillus thuringiensis genome using GS FLX-titanium
표 GUS stained Arabidopsis treated with two isolates show increasement of DR5::GUS included auxin signaling. Dark blue means more auxin responsive gene increase than clearer one.
표 Enterobacter sp. 1-1 and 6-2 growth test
표 Salkowski’s assay to detect bacterial IAA by growth time. Increasing bacterial IAA shows pink color.
표 Phenotype changes of Arabidopsis treated bacteria on plate.
표 Filter paper assay to examine bacterial volatile organic compounds effect on Arabidopsis
표 Quantification of Arabidopsis root development
표 The expression levels of representative auxin-responsive genes of Arabidopsis treated with bacteria.
표 Analysis of DR5:GUS expression pattern and its regulation by EB1.
표 The salt resistant fungi isolated from the inside root tissues of halophytic plants
표 The promotion of growth and alleviation of salt stresses in sudan grass treated with Fsh7
표 The growth pattern of sudan grass in presence and absence of Fsh7
표 The increased salt tolerance of sudan grass treated with Fsh7 spore suspension.
표 The treatment of VOC driven from M. fragilis Fsh7 to Arabidopsis
표 The treatment of VOC driven from M. fragilis Fsh7 to tomato
표 The influence of VOC from M. frgilis Fsh7 on Sudangrass
표 Scanning electron microscopy (SEM) of VOC based interaction studies between Fsh7 and plant pathogen S. sclerotiorum
표 Plant growth promoting rhizobacteria isolated form Blue pea
표 Plant growth-promoting attributes in culturable nodule from Blue pea
표 Phylogenetic relatoinships of root nodule endophyte bacteria Paenibacillus polymyxa RCP6 (GU369972) based on 16S rRNA sequence and related nearest neighbor sequences.
표 Plant growth promoting traits in root nodule strain P. polymyxa RCP6 (GU369972).
표 Effect of seed bacterization with P. Polymyxa RCP6 (GUB) on (a) seed germination and (b) early vegetative growth of Clitoria purpurea L., Arachis hypogea L., Sesamum indicum L., Brassica juncea L. under pot trials (20DAS).
표 The effect of Pantoea ananatis on growth and salt tolereance of Arabidopsis.
표 The effect of Salanicola peritrichatus on growth and salt tolereance of Arabidopsis.
표 Average fresh weights(A) and dry weights(B) of Arabidopsis untreated(A) or treated with Pantoea ananatis(B) under 0 mM and 200 mM NaCl.
표 Average fresh weights(A) and dry weights(B) of Arabidopsis untreated(A) or treated with Salanicola peritrichatus(B) under 0 mM and 200 mM NaCl.
표 Gene expression profiles of representative salt stress responsive genes in Arabidopsis in presence or absence of P. ananatis sp. 103V (P103V).
표 The various PGP activity of Trichoderma spp. Gur1
표 The plant growth promoting effect of T. cerinum spp. Gur1
표 Effect of bacterization on vigor index (■) and fresh weight (□) of barley paper towel containing 150 mM of NaCl
표 Effect of bacterization on vigor index (■) and fresh weight (□) of wheat (cv Jokyung) in paper towel containing 200 mM of NaCl.
표 Effect of bacterization on vigor index (■) and fresh weight (□) of barley (cv Saessal) in soil containing various concentrations of NaCl.
표 Characteristic of heavy metals tolerance using minimal inhibitory concentration test
표 Phylogenetic tree analysis of B. psedomycoides JH 2-2 (●) and C. freundii JH 11-2 (●) 16S-rDNA
표 Distribution and uptake of Cd (II) by C. freunii strain JH 11-2
표 Bioremediation mechanisms schematic by cell adsorption and transporter protein.
표 FT-IR analysis of C. freundii JH 11-2 strain producing EPS (B), cell membrane of growth in with 100 mg L-1 cadmium (A) and without cadmium (C). Arrow has shown wavelength of IR peaks respectively.
표 Influence of initial pH on Cd (II) species and removal capacity by C. freundii JH 11-2 strain
표 Estimation of Cr (VI) concentration with changing time by inoculation of B. pseudomycoides JH 2-2.
표 Influence of chromium-resistant bacteria on seed germination
표 Effect of inoculation with B. pseudomycoides JH 2-2 on Brassica juncea L. shoot (A) and root (B) length at 10, 30 and 50 mg Cr (VI) L-1.
표 The influence of L-tryptophan (L-trp) on B. pseudomycoides JH 2-2 growth and IAA production without Cr(VI) stress (A) and with 10 mg Cr(VI) L-1 stress (B).
표 Growth rate and IAA production of JBLS202 in LB at various salt concentrations.
표 Growth rate and Indole production of JBLS202 in LB at various salt concentrations.
표 SPME-GC/MS chromatogram profiles of VOCs emitted from Proteus vulgaris JBLS202 cells.
표 Effect of commercial indole on the growth of cabbage.
표 Production of extracellular indole by Proteus vulgaris JBLS202 cells.
표 Effect of bacterization on the growth of cabbage.
표 GC/MS profile of headspace volatiles from cabbage seedlings bacterized with Proteus vulgaris JBLS202 cells.
표 Effect of JBLS30 concentration on growth of Chinese cabbage.
표 Effect of bacterization on growth of Chinese cabbage
표 Effect of Pseudomonas sp. JBLS30 bacterization on the growth of Kimchi cabbage.
표 Effect of Pseudomonas sp. JBLS30 bacterization on the growth of Kimchi cabbage.
표 Growth rate and Indole production of JBLS30 in LB at various salt concentrations.
표 The isolation of Trichoderma Gur1 strain and its taxonomic identification
표 The phenotype of Arabidopsis treated with Gur1 w/ or w/o F. oxysporum.
표 The various growth parameters of Arabidopsis treated with Gur1 w/ or w/o F. oxysporum.
표 The phenotype of Arabidopsis treated with Gur1 w/ or w/o NaCl.
표 The concentration of cellular compound which reflect the oxidative status in Arabidopsis
표 The expression of defensive- and stress responsive marker genes under Gur1 treated conditions.
표 The effect of EJ01 on the growth and salt stress tolerance in tomatoes
표 The colonization of EJ01 containing GFP as a fluorescent reporter in Arabidopsis roots
표 The effects of EJ01 on the growth and salt stress resistance of Arabidopsis
표 The expression profiles of well-documented stress responsive genes of Arabidopsis within six hours after treatment of EJ01
표 The measurement of ROS scanvenging activities in tomato treated with EJ01
표 Visible changes in growth performance of Arabidopsis thaliana Col-0 seedlings stress after 14 d of exposure to volatiles from Alcaligenes faecalis JBCS1294.
표 Growth responses of Arabidopsis thaliana Col-0 and its mutant lines to volatiles from JBCS1294
표 The effect of Alcaligenes faecalis JBCS1294 volatiles on growth performance of Arabidopsis thaliana Col-0 and mutant lines under salt stress.
표 Influence of phytohormone inhibitors on growth of Arabidopsis thaliana Col-0 plants to volatiles from JBCS1294
표 The effect of Alcaligenes faecalis JBCS1294 volatiles on growth performance of hormone inhibitor treated Arabidopsis thaliana under salt stress.
표 Physicochemical properties of soil
표 Growth kinetics of Herbaspirillum sp. GW103 in the presence of various chemical chelators
표 Treatment effects on the concentration of Pb in Zea mays. Error bar indicate standard deviation of means, where absent, bar fall within symbols.
표 Bacterial heavy metal resistance genes and their specific primers and functions.
표 PCR amplification of ArsB and ACR3(1 and 2) genes using gene specific primers.
표 PCR amplification of PbrA and PbrT genes using gene specific primers.
표 PCR amplification of metallothionein gene (bmtA) using gene specific primers.
표 Physico-chemical properties of mine waste soil
표 Phylogenetic tree of fungal isolates. Bootstrap values 1000 replicates.
표 Heavy metal tolerance of selected rhizosphere bacterial isolates
표 metal tolerance of selected endophytic bacterial isolates
표 18S rDNA analysis of selected fungi and their closest similarity sequences from NCBI
표 Plant growth promoting traits of selected rhizosphere bacterial isolates
표 Plant growth promoting traits of selected endophytic bacterial isolates
표 Plant growth promoting traits of fungal isolates
표 Role of bacterial consortium on maize plant root length and root weight after 45 days of growth.
표 Role of bacterial consortium on maize plant shoot height and weight.
표 Effect of bacterial consortium on physico-chemical and biochemical properties
표 Role of selected fungi on Sorghum grass shoot height after 9 weeks of grass growth.
표 Role of selected fungi on Sorghum grass root dry weight after 9 weeks of growth.
표 Role of selected fungi on Sorghum grass shoot dry weight after 9 weeks of growth.
표 Accumulation of heavy metals in root and shoot tissue of Sorghum grass inoculated with PDR-16, PDR-28 fungi and A+B
표 Physico-chemical characteristics properties of mine waste soil inoculated with PDR-16, PDR-28 fungi and A+B
표 Composition of stuart
표 Urease activity of U1 and U2
표 Heavy metal tolerance of ureolytic bacteria.
표 PGP(Plant growth promoting) properties of U1 and U2.
표 Growth and urease activity of U2 at different pH conditions.
표 Concentrations recovered after treatment with different pH values(4, 6 and 8) at heavy metal initial concentration.
표 Concentrations recovered after treatment with different temperatures values(25, 30 and 37℃) at heavy metal initial concentration.
표 Growth and urease activity at different temperatures.
표 Concentration recovered after treatment with initial heavy metals concentration (As 20, Cu 10, Pb 75 and Zn 30 mg kg-1).
표 Concentrations recovered after treatment with initial heavy metals concentration (As 30, Cu 15, Pb 100, and Zn 40 mg kg-1).
표 A, control sample(bacteria was grown LB broth in the absence of heavy metals) and B, bacteria was grown in heavy metal amended soil extract medium.
표 XRD spectra conforming calcite precipitation in soil extract medium sample induced by U2
표 The selection of salt tolerant metagenomic fosmid clones and experimental procedures to identify the single gene level of salt tolerant genes
표 The growth curve of E. coli containing metagenome-originated ADH in presence of various salt concentrations.
표 The salt tolerance of transgenic lines expressing metagenome-originated ADH (aldehyde dehydrogenase).
표 Genes of which expression were up-regulated by the treatement of PGPR P. fluorescens FPT9601-T5
표 The amplication of PCR1, PCR2, and CRK4 from Arabidopsis leaf tissue
표 The expression profiles of PCR1 upon the treatment of various hormones and stress agents.
표 The root architecture of Arabidoposis treated with volatile compounds
표 The morphological changes of lateral roots according to various dosage of 2,3-butanediols.
표 The heat map and gene ontology terms of differentially regulated genes (DEGs) according to the exposure to 2,3-butanediol for various time courses.
표 The categorized clusters of differentially expressed genes (DEGs) based on the similarities of changes of expression patterns.
표 The enriched gene ontology terms of each clusters of differentially expressed genes.
표 Protein-protein interaction map of At2g40340 (DREB2B)
표 Quantitative measurement of the expression levels of bacterial genes under salt stress in B. subtilis GB03.
표 The construction of Pseudomonas fluorescenes gDNA library. A. pGBKT7RSV5 and the genomic DNA purifed from P. fluorescenes were respectively digested by BamHI and StuI. B.
표 The overview of our yeast two hybrid screening using two DNA libraries between P. fluorescences and Arabidopsis.
표 The PCR amplification using the plasmid DNAs purified from isolated colonies.
표 P. fluorescens와 애기장대간의 잠정적 물리적 상호작용 인자
표 Plant growth promotion effect of DMDS on Arabidopsis. a. Freshweight, b. Leaf area, c. Chlorophyll content(P<0.05).
표 The alterations in root architecture of Arabidopsis according to DMDS treatment.
표 The enhancement of auxin signaling in Arabidopsis treated with DMDS
표 Alteration of root architecture of Arabidopsis auxin signaling mutants according to DMDS treatment.
표 The antifungal activity of DMDS on PDA agar media against various fungal phytopathogens.
표 Volatile organic compounds produced by Alcaligenes faecalis JBLS1294 as measured by GC/MS
표 SPME-GC/MS chromatogram profiles of VOCs emitted from Alcaligenes faecalis JBLS1294.
표 Change in proline content after 14 days of exposure to VOCs from JBCS1294.
표 Changes in gene expression in hormonal pathways and specific key ion transporters in root and shoot tissues after exposure of 10-day-old Arabidopsis seedlings under salt stress to volatiles from JBCS1294 for 24 h.
표 The deduced amino acid sequences of a P. fluorescens ACCd clone, PfACCd.
표 The increased root growth of transgenic Arabidopsis expressing PfAcdS genes
표 The reduced sensitivity to ACC in transgenic lines expressing PfAcdS genes.
표 The protein degradation of PfAcdS in transgenic Arabidopsis.
표 Specific hybridization of polyclonal anti-ACCd antibody. a Western blot of recombinant ACC deaminase.
표 Detection of AcdS in isolated soil microorganisms by Western blotting and the comparison with biochemical analysis.
표 MIC of isolate AGB-1.
표 Plant growth-promoting characteristics and heavy metal tolerance of P. fluorescens AGB-1.
표 Water-soluble Cu contents in control (without bacteria inoculated) and AGB-1 inoculcated flaks at 48h of growth period.
표 Influence of isolate ADB-1 on M. sinensis growth in heavy metal mine tailing soil.
표 Effect of isolate AGB-1 on M. sinensis height.
표 Role of AGB-1 inoculation on M. sinensis culms number.
표 Effect of AGB-1 inoculation on numebr of M. sinensis leaves production.
표 Accumulation of heavy metals in root and shoot tissue of M. sinensis inoculated with AGB-1.
표 AM fungal colonization in control and AM inoculated plant roots.
표 Influence of AM fungi on plant root and shoot length.
표 Role of AM fungi on plant dry weight production.
표 Effect of AM fungal inoculation on the production of chlorophyll content.
표 As accumulation in AM inoculated and non-inoculated A. firma plants.
표 Effect of AM fungi on Cd accumulation of A. firma.
표 Role of AM fungi on Pb accumulation of A. firma.
표 Zn accumulation in AM fungal inoculated and non-inoculated A. firma.
표 GRSP content in soil inoculated with AM fungi and control.
표 Growth-promoting characterization of PDR-4 and PDR-16.
표 Influence of fungi on sorghum-sudangrass biomass production.
표 Effect of fungi on sorghum-sudangrass chlorophyll.
표 Accumulation of heavy metals in root and shoot tissue of sorghum-sudangrass inoculated with PDR-4 and PDR-16, alone and in combination.
표 Growth-promoting characteristics and heavy metal tolerance of Trichoderma virens PDR-28 isolated from mine tailing soil containing heavy metals.
표 Influence of PDR-28 on dry weight of maize.
표 Role of PDR-28 on maize reducible-non reducible sugars, total sugars, total starch and protein yield.
표 Accumulation of heavy metals in roots and shoots(dry biomass) of maize grown in soil inoculated with PDR-28.
표 Auxin producing in different concentration Zn (II) and Cu (II) (10, 30, and 50 mg L-1) by B. sp. JH 2-2 strain
표 Auxin producing in different concentration Pb (II) and As (III) (10, 30, and 50 mg L-1) by B. sp. JH 2-2 strain
표 Influence of exogenous Indole-3-acetic acid on growth of B. sp. JH 2-2 with Cu (II) stress
표 Inactivation of indole-3-acetic acid (1 mM) by UV-C (254 nm) treatment with changing bulb number.
표 Influence of UV254 irradiation on indole-3-acetic acid form by HPLC analysis. Standard indole-3-acetic acid (A); UV 254 irradiation products (B).
표 Pb and Zn uptake in shoots and roots of Zea mays L. with addition of Herbaspirillum sp. GW103.
표 Physicochemical properties of mine soil
표 The survival of GFP tagged Herbaspirillum sp. GW103 in Cu contaminated mine soil.
표 Primers employed expression of arsC genes in this present study
표 The relative expression of arsenic reductase genes (a) arsC1, (b) arsC2, (c) arsC3 and (d) arsC4.
표 the results of identified bacteria isolated from contaminated mine soils.
표 Removal percentage of As in control, SH-1, SH-2, SH-3and SH-4 treated mine soil leachate in different time interval. Error bars indicate standard deviation of means, where absent, bars fall within symbols
표 Removal percentage of Pb in control, SH-1, SH-2, SH-soil leachate in different time interval. Error bars indicate standard d3eavniadt ioSnH -of4 mtereaantse, d wmheinree absent, bars fall within symbols.
표 Removal percentage of Zn in control, SH-1, SH-2, SH-soil leachate in different time interval. Error bars indicate standard d3eavniadt ioSnH -of4 mtereaantse, d wmheinree absent, bars fall within symbols
표 tRee mino vdaiflf epreerncte ntitmagee inotfe rCvua l.i nE rcroonr trboalr, s SiHnd-ic1a, teS Hs-ta2n,d aSrdH -d3eavniadt ioSnH -of4 mtereaantse, d wmheinree absent, bars fall within symbols.
$the experimental methods of soil sequential extraction to confirm fraction of heavy metals in soil.$ 표 the experimental methods of soil sequential extraction to confirm fraction of heavy metals in soil.
$Distribution of As fractions in control and bacterial isolates treated soil. Error standard deviation of means, where absent, bars fall within symbols.$ 표 Distribution of As fractions in control and bacterial isolates treated soil. Error standard deviation of means, where absent, bars fall within symbols.
$Distribution of Pb fractions in control and bacterial isolates treated soil.$ 표 Distribution of Pb fractions in control and bacterial isolates treated soil.
$Distribution of Zn fractions in control and bacterial isolates treated soil.$ 표 Distribution of Zn fractions in control and bacterial isolates treated soil.
$Distribution of Cu fractions in control and bacterial isolates treated soil.$ 표 Distribution of Cu fractions in control and bacterial isolates treated soil.
$X-ray diffractogram of mine soils.$ 표 X-ray diffractogram of mine soils.
표 The lists of useful microbial resources studies in this project (2012~2016)
표 The quantitative comparison of number of publishment among departments in similar field.

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