초록

당초 목표인 9편의 국외 SCI 논문, 5건의 특허 출원을 훨씬 초과한 25편의 국외 논문 게재, 7건의 특허 출원, 9건의 특허 등록을 달성하였음. 또한 선정당시 목표에 없었던 기술 실시계약을 1건 체결하였음. 또한 본 과제의 연구 결과를 48개 국내외 학회에서 발표하는 등, 아주 우수한 연구 개발 성과를 도출함.

(출처 : 보고서 요약서 3p)

Abstract ▼

Purpose&contents
In this research project, we investigated genetic, epigenetic and immunological factors involved in development and progression of gastric cancer.
1. Identification of genetic factors for gastric cancer: we identified genetic associations of maspin, ANKRD9 and TFAM genes with

Purpose&contents
In this research project, we investigated genetic, epigenetic and immunological factors involved in development and progression of gastric cancer.
1. Identification of genetic factors for gastric cancer: we identified genetic associations of maspin, ANKRD9 and TFAM genes with gastric cancer susceptibility and investigated their roles in gastric cancer.
2. Identification of epigenetic factors for gastric cancer: we investigated the roles of histone methylation modifiers including EZH2, UTX and G9a in gastric cancer.
3. Identification of inflammatory factors for gastric cancer: we have established protocols to analyze immune cells in the gastric cancer model mice and investigated the role and regulatory mechanisms of the inflammatory factors involved in gastric cancer progression.

Result
1. Identification of genetic factors for gastric cancer
- We identified genetic association between ANKRD9 and Korean susceptibility to gastric cancer and uncovered its roles in gastric cancer cells. In addition, we identified binding partners of ANKRD9 protein and uncovered its novel biochemical function.
- We characterized functional roles of the mitochondrial transcription factor TFAM in gastric cancer cells and identified its downstream target genes.
- We identified genetic association between maspin gene and Korean gastric cancer risk and uncovered its role as a suppressor of gastric cancer.
- We investigated molecular mechanisms of various gastric cancer-related factors including Argonaute and Capicua.
2. Identification of epigenetic factors for gastric cancer
- We discovered an inverse relationship between cellular levels of histone methyltransferase EZH2 and histone demethylase UTX.
- We uncovered methylation of RUNX3 by G9a.
- We identified genetic association of EZH2, UTX and JMJD3 with Korean susceptibility to gastric cancer.
- We identified statistical association of EZH2, UTX and JMJD3 expression levels with overall survival rate of gastric cancer patients.
- We demonstrated suppressive role of UTX in colony-forming activity of gastric cancer cells
3. Identification of inflammatory factors for gastric cancer
- We have established a xenograft mouse model of gastric cancer.
- We have developed protocols to analyze immune cells in gastric cancer model mice.
- We investigated mechanisms for interferon induction regulating cancer progression.
- We investigated functions and structures of various inflammatory factors regulating cancer development, such as RIG-I ligand, IRAK1, NF-kB and BP16.

Expected Contribution
1. Providing a platform to develop biomarkers for gastric cancer.
2. Developing potential anti-cancer therapeutic drugs targeting histone modifying enzymes
3. Providing reference protocols for analyzing various immune cells and factors regulating other cancer types.
4. Applicable to studies on other types of cancer.

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 목차 ... 4
• 연구계획 요약문 ... 5
• 연구결과 요약문 ... 6
• 한글요약문 ... 6
• SUMMARY ... 7
• 연구내용 및 결과 ... 8
• 1. 연구개발과제의 개요 ... 8
• 2. 국내외 기술개발 현황 ... 9
• 3. 연구수행 내용 및 결과 ... 11
• 4. 목표달성도 및 관련분야에의 기여도 ... 68
• 5. 대표적 연구실적 ... 74
• 6. 연구결과의 활용계획 ... 75
• 7. 연구과정에서 수집한 해외과학기술정보 ... 75
• 8. 참고문헌 ... 77
• 9. 연구성과 ... 79
• 10. 연구기자재 현황 및 활용 ... 97
• 11. 기타사항 ... 97
• 별첨1 대 표 연 구 성 과 ... 98
• 별첨2 기초연구실 참여 인력 현황 ... 108
• 끝페이지 ... 108