초록 ▼

표적화 된 대사체 분석을 통하여 삼중음성 유방암에서 PIP2（phosphatidyl inositol di-phosphate)를 정량적으로 분석하는데 성공함. 또한Eicosanoids를 체계적으로 분석하여 유방암에서 Prostaglandin의 일종인 PGD2와 PGE2가 miR-155의 발현양과 상관관계가 있음을 발견 함. 이러한 상관관계의 기작을 이해하기 위해 miR-155의 타겟 유전자를 조사하였고 그 결과 Myc과 p85 (PI3K regulatory subunit) 그리고 C/EBP-beta등이 miR-155에 의하여 조절되는

표적화 된 대사체 분석을 통하여 삼중음성 유방암에서 PIP2（phosphatidyl inositol di-phosphate)를 정량적으로 분석하는데 성공함. 또한Eicosanoids를 체계적으로 분석하여 유방암에서 Prostaglandin의 일종인 PGD2와 PGE2가 miR-155의 발현양과 상관관계가 있음을 발견 함. 이러한 상관관계의 기작을 이해하기 위해 miR-155의 타겟 유전자를 조사하였고 그 결과 Myc과 p85 (PI3K regulatory subunit) 그리고 C/EBP-beta등이 miR-155에 의하여 조절되는 것을 규명 함

( 출처: 보고서 요약서 3p )

Abstract ▼

□ Purpose&Contents
Purpose : This project aims to identify metabolite biomarker for breast cancer, using LC-MS/MS technology.
Contents : We performed targeted metabolomics for phosphatidyl inositiol phosphate (PIPs) and prostaglandin (PG), to reveal its role in breast cancer.
For this, huma

□ Purpose&Contents
Purpose : This project aims to identify metabolite biomarker for breast cancer, using LC-MS/MS technology.
Contents : We performed targeted metabolomics for phosphatidyl inositiol phosphate (PIPs) and prostaglandin (PG), to reveal its role in breast cancer.
For this, human breast cancer cell lines and tumor specimen was used. To understand how the change of metabolism occur, we investigated correlation between miR-155 and PGs.

□ Results
1. Publication
We successfully published two SCI(E), international research papers. The first one revealed miR-155 regulated the level of thiamine, a cofactor of energy metabolic enzymes. This is published in Cancer Letters. Second one addressed the role of miR-155 in the recruitment of MDSC（Myeloid Derived Suppressor Cells) in tumor microenvironment, which is regulated by C/EBP-beta and cytokine production
2. Patent
Based on the finding that miR-155 regulate thiamine level, we file a patent titled as development of multi-biomarker for diagnosing cancer (patent number １０－２０１４－０１３５７６９)
3. Education
Two student who involved in this project received their master degree by the support of this grant. One more student will receive his MS degree on the coming Feb.
4. Other research products
We are investigating the role of miR-155 in glucose metabolism via multiple transcription factor regulation and preparing a manuscript. Also another project is ongoing to address the mechanism by which miR-155 regulate PGE2 and PGD2 level.

□ Expected Contribution
★ There are four points that our results contribute to in future
- First, our study prove the targeted metabolomics method cost less but more effective to identify biomarker useful for diagnosis or prognosis prediction
- Second, considering the importance of PIPs and PG, our results will be expandable to other types of cancer
- Third, our study not only reveal novel metabolite markers but also identify enzymes that is involved in the metabolism of this metabolite. This will lead us to develop agent that target these enzymes as a cancer drug candidate.
- Fourth, the metabolite identified in this study will be useful in combination with other genetic or proteomic markers to compensate the limitation of each markers each other.

( 출처: SUMMARY 5p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 5
• 영문목차 ... 6
• 목차 ... 6
• 1. 연구개발과제의 개요 ... 7
• 1-1. 연구개발 목적 ... 7
• 1-2. 연구개발의 필요성 ... 8
• 1-3. 연구개발 범위 ... 9
• 2. 국내외 기술개발 현황 ... 11
• 3. 연구수행 내용 및 결과 ... 15
• 4. 목표달성도 및 관련분야 기여도 ... 25
• 4-1. 목표달성도 ... 25
• 4-2. 관련분야 기여도 ... 25
• 5. 연구결과의 활용계획 ... 26
• 6. 연구과정에서 수집한 해외과학기술정보 ... 28
• 7. 연구개발성과의 보안등급 ... 29
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 29
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 29
• 10. 연구개발과제의 대표적 연구실적 ... 30
• 11. 기타사항 ... 30
• 12. 참고문헌 ... 31
• [별첨4] 실적 증빙자료 ... 32
• 끝페이지 ... 38