초록

염류 및 고온 저항성 유전자원을 선발하기 위한 기내검정법을 구축하고 이를 활용하여 전사체와 단백질의 변화를 확인하였다.
스트레스 정도를 판단할 수 있는 생리학적 지표(엽록소 형광반응)와 저항성 바이오마커를 동시에 비교하여 보다 정확한 검정체계를 완성하였다. 염류와 고온에 저항성을 평가할 수 있는 바이오마커는 향후 목적형질을 선발할 수 있는 시스템으로 활용할 계획이다.

(출처: 요약서 2p)

Abstract ▼

□ Purpose&Contents
In order to establish the foundations of molecular breeding of ginseng, it will be necessary to actively utilize transcriptome and proteome analyses to uncover more diverse breeding materials, and to construct and use information about molecular markers, genes, and proteins rel

□ Purpose&Contents
In order to establish the foundations of molecular breeding of ginseng, it will be necessary to actively utilize transcriptome and proteome analyses to uncover more diverse breeding materials, and to construct and use information about molecular markers, genes, and proteins related to desired characteristics. Therefore, in this study, in order to breed varieties that are resilient to environmental stresses such as salt and high temperature, we aimed to develop basic technologyto rapidly and accurately search for useful characteristics. First,we constructed a testing method to screen for resources in high salinity and high temperature conditions, and examined changes in the transcriptome and proteins. We selected 10genes with high expression under salt treatment, and confirmed that these were mostly ion transporters that respond to external stress and genes that reduce levels of intracellular toxins. Among proteins, we identified 3 proteins with increased expression in both 3-, 4-, and 5-year-old ginseng (myo-inositol-1-phosphate synthase, glycosyl hydrolase family 14, glycoside gydrolase family 17). We prepared qPCR primers based on the 10 selected genes, and were able to use these to compare relative salt tolerance. For the 3 selected proteins, we prepared specific antibodies that bind to these proteins and used these to assess salt tolerance. In order to develop DNA markers in the‘G07006’ line, which was selected as a salt tolerant line, we performed whole chloroplast genome sequencing, and found that the salt tolerant line could be distinguished by 5 specific SNPs.

For the testing method to screen heat tolerant resources, we first screened and compared chlorophyll fluorescence under heat treatment (35ºC, 72 hours). Then, we selected transcriptomes and proteins that showed changes, and used these as biomarkers. As a result, we selected 3 genes related to abiotic stress and signal transduction, and assessed tolerance based on the available genetic resources. Among proteins, when ginseng was exposed to high temperatures, we identified changes in proteins associated with G-protein and calcium signaling pathways. Of these, 3 proteins were strongly correlated with transcriptome expression (myo-inositol-1-phosphate synthase, glycosyl hydrolase family 14, glycoside gydrolase family 17), and these were selected as the final markers

□ Results
In this study, for the first time, we developed an in vitro screening method to test for heat and salt tolerance. In addition, we developed biomarkers based on the genes and proteins that responded to stress (salt, heat), and used these to select candidate tolerant strains. The SOD-antibody for testingssalt tolerance is currently patented, and the related genes andproteins have all been registered with the NCBI. In order to improve gene prediction, we used the single molecule-based Pac Bio RS II device to read long sequences of over 2–6 kb.
Thus, by identifying 91,345 unigenes from the full-length sequencing read set, we greatly improved prediction of gene functions.

□ Expected Contribution
Due to the biological characteristics of ginseng (1 generation every 4 years), it has taken a long time to screen ginseng breeding resources. The salt and heat tolerance biomarkers in developed in this study provide data to support the future cultivation of new varieties, by reducing the time required forscreening, and enabling the characteristics of genetic resources to be evaluated. This can be used as a single system for molecular breeding and precision breeding of ginseng.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 보고서 요약서 ... 2
• 국 문 요 약 문 ... 3
• Summary ... 5
• 목차 ... 7
• 제 1 장 연구 개발 과제의 개요 ... 8
• 제1절 연구 개발 목적 ... 8
• 제2절 연구 개발의 필요성 ... 8
• 제3절 연구 개발 범위 ... 8
• 제 2 장 연구 수행 내용 및 결과 ... 10
• 제 3 장 목표달성도 및 관련분야 기여도 ... 41
• 제1절 목표대비 달성도 ... 41
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 42
• 제 4 장 연구 결과의 활용 계획 ... 43
• 제 5 장 연구 개발 결과의 보안 등급 ... 44
• 제 6 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 45
• 제 7 장 연구개발과제의 대표적 연구실적 ... 46
• 제 8 장 기타사항 ... 48
• 제 9 장 참고문헌 ... 52
• 끝페이지 ... 56