[보고서]고추 병해충(진딧물, 탄저병) 동시방제 미생물제 개발

한지희

보고서 정보

주관연구기관

국립농업과학원
National Institute of Agricultural Sciences

연구책임자

한지희

참여연구자

이상엽 , 김다연 , 안성호 , 김재윤 , 김영철 , 강범용 , 이용성 , 박주연 , 김철홍 , 한송희 , 하헌익

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2019-02

과제시작연도

2018

주관부처

농촌진흥청
Rural Development Administration(RDA)

등록번호

TRKO201900016025

과제고유번호

1395055912

사업명

친환경안전농축산물생산기술(R&D)

DB 구축일자

2019-10-19

키워드

곤충병원곰팡이.복숭아혹진딧물.탄저병.고추.제제화.entomopathogenic fungi.green peachaphid.anthracnose.pepper.Simultaneous control.

DOI

https://doi.org/10.23000/TRKO201900016025

표 Primers used in this study
표 Pf185 처리에 의한 고추 복숭아혹진딧물 방제효과
표 Pf185, Pf212의 복숭아혹진딧물 예방효과 (선발 균주 처리 24시간 후 진딧물 접종)
표 Pf185 포자 농도별 복숭아혹진딧물 기피효과
표 Pf185 배양여액 농도별 복숭아혹진딧물 기피효과
표 Pf212 포자 농도별 복숭아혹진딧물 기피효과
표 Pf212 배양여액 농도별 복숭아혹진딧물 기피효과
표 Pf185, Pf212 균주에 의한 고추탄저병 예방효과 (선발 균주 처리 24시간 후 탄저병균 처리) (A) : 포자현탁액, (B) : 배양여액
표 Pf185, Pf212 균주에 의한 고추탄저병 방제효과 (탄저병→4시간 후 선발 균주 처리)
표 Pf185, Pf212 균주에 의한 고추탄저병 방제효과 (탄저병→24시간 후 선발 균주 처리)
표 Pf185, Pf212 처리에 따른 탄저병균 생육저지 효과
표 Pf185 균주가 진딧물에 미치는 영향 복숭아혹진딧물(A, B), 목화진딧물(C, D)
표 Pf185(A,B), Pf212(C,D) 균주의 탄저병원균에 대한 기생활성(X400) 왼편 : Pf185, Pf212, 오른편 : 고추 탄저병원균
표 Pf185, Pf212 균주의 protease, chitinase 활성 검정
표 Pf185, Pf212 균주의 오이총채벌레 방제효과
표 Pf185, Pf212 1차 액상 시제품의 진딧물 방제효과(leaf disc)
표 Pf185, Pf212 1차 액상 시제품의 진딧물 방제효과(포장 포트검정)
표 Pf185, Pf212 시제품 처리에 의한 진딧물 방제효과 (포장)
표 Pf185, Pf212 포자 현탁액 처리에 의한 진딧물 방제효과 (포장)
표 Pf185, Pf212 시제품의 고추 탄저병 예방효과(포장)
표 Pf185, Pf212 시제품 처리에 따른 탄저병 예방효과(포장)
표 Pf185, Pf212 시제품의 고추 탄저병 치료효과(포장)
표 Pf185균주 포자의 작물(고추) 내 생존율 A, C, E : field B, D, F : potted plant in tent
표 고추 내생 곰팡이
표 선발 Pf185 균주의 예방처리에 의한 복숭아혹진딧물 방제 효과(포트) (A)~(D) : 미생물 시제품 처리 1-4주 후
표 곤충병원곰팡이의 오이총채벌레 방제효과
표 선발 균주의 총채벌레 방제효과 검정
표 추가 선발 균주의 병해충 방제효과
표 최종 선발 균주의 병해충 방제효과
표 Isaria javanica FT333의 계통도
표 Isaria javanica FT333의 형태
표 I. javanica FT333의 고추 탄저병균에 대한 실내 방제 효과
표 최종 선발 Isaria javanica FT333균주의 고추 탄저병 방제 효과(실내검정)
표 Phylogenetic trees based on the internal transcribed spacer (ITS, A) and partial ß-tubulin (TUB2, B) sequences of isolates pf185 and pf212 and ex-type strains of the Isaria genus (Supplemental Table 1). The phylogenetic trees were constructed using the neighbor-joining method and Kimura’s two-parameter model. The numbers at nodes indicate consensus bootstrap values based on 1,000 replications. Scale bar = 0.01 substitutions per nucleotide position. Sequences from Metarhizium anisopliae and M. brunneum were used as the outgroups for ITS and ß-tubulin sequence analyses, respectively. Abbreviations: ARSEF, Agricultural Research Service Collection of Entomopathogenic Fungal Cultures (Ithaca, NY, USA); CBS, Centraal Bureau voor Schimmelcultures (Utrecht, The Netherlands)
표 Comparison of the size and shape of conidia from Isaria javanica isolates pf185 and pf212
표 Morphology of the conidia and phialides of Isaria javanica isolates pf185 and pf212. Phialides with developing conidial chains of I. javanica pf185 (A) and I. javanica pf212 (B). Typical shapes of individual conidia of I. javanica pf185 (C) and I. javanica pf212 (D). Scale bars represent 10 mm. The images are representative of two independent observations with at least 40 conidia and 70 phialides
표 Growth inhibition of four plant pathogens caused by Isaria javanica pf185 and pf212 when grown in dual cultures on potato dextrose agar. Fo, Fusarium oxysporum; Cg, Colletotrichum gloeosporioides; Pc, Phytophthora capsici; and Rs, Rhizoctonia solani. (A) Error bars represent the mean standard error of 10 total replicates per treatment in two independent experiments. P -values are provided for the significant differences in growth under different treatments (Student’s t-test, α < 0.05). (B) The images were obtained at 7 d after co-cultivation. The fungal pathogen was inoculated on the left-hand side of the plate, and I. javanica pf185 or pf212 on the right-hand side. The images are representative of two independent experiments with five plates for each experiment
표 The percentage cumulative mortality of second-instar green peach aphid nymphs, following treatment with spore suspensions of I. javanica pf185. The nymphs were treated with spores at concentrations of 106(6), 107(7), and 108(8) spores/mL. Error bars represent the mean standard error of three replicates per treatment in two independent experiments. Data were analyzed using ANOVA (P < 0.05), and if the F test was significant, differences were further elucidated using Duncan’s multiple range test. Different letters indicate significant differences at P < 0.05 at each time point
표 Probit analysis of detached-leaf bioassays for estimation of changes in the lethal times of Isaria javanica pf185 at different spore concentrations for second-instar nymphs of the peach aphid
표 1H (500MHz) and 13C (125MHz) NMR spectral data of dibutyl succinate in CD3OD
표 (A) Fractionation procedure for the purification of the antifungal compound from the culture filtrate of I. javanica pf185. (B) The spectrum detected at 210 nm from high performance liquid chromatography (HPLC) of the extract from TLC that had antifungal activity
표 The 1H (A) and 13C (B) nuclear magnetic resonance (NMR) spectra of the purified metabolite, dibutyl succinate, produced by I. Javanica pf185 and dissolved in CD3 OD/CDCl3 (1:1; v/v) at 500MHz for 1H and 125MHz for 13C at room temperature
표 Liquid chromatography mass spectrometry (LC-MS) spectrum of the compound purified through high liquid chromatographic purification showing main ion peaks at [M+H]+atm/ z 231.1 in the positive ESI scan. Chemical structure and significant correlation in 1H-1H COSY (solidlines), and HMBC (arrows) spectra of the purified metabolite, dibutyl succinate, produced by I. javanica pf185
표 COSY, HMBC, and HSQC spectra of nuclear magnetic resonance (NMR) spectroscopy of the purified antifungal compound in methanol-d4 at 500 MHz
표 High performance liquid chromatography (HPLC) of the extract from Isaria javanica pf185 and authentic dibutyl succinate. Similar peaks in both samples at retention time of 14.6 min were detected
표 The antifungal activity of dibutyl succinate against C. acutatum. (A) Inhibition of mycelial growth of C. acutatum by defined concentrations of authentic dibutyl succinate. Images were taken 7 d after treatment. (B) Inhibition of conidial germination after 6 h of incubation. At least 100 spores for each treatment were examined. Spores that produced germ tubes longer than their diameter were considered to have germinated. Each experiment was repeated twice in triplicates per treatment. Means and standard errors for two independent experiments are shown. Different letters indicate statistically significant differences (P < 0.05) by Duncan’s multiple test
표 Probit analysis of detached-leaf bioassays to estimate median lethal concentrations (LC50) and times (LT50) of the authentic dibutyl succinate
표 Effects of temperate on fungal growth and antifungal activity in Isaria javanica pf185. The onidial production and antifungal activity of I. javanica pf185 grown at various temperatures were assessed after 7 days of incubation in liquid and solid agar plates using Sabouraud dextrose medium. Data are the means ± standard deviations from two independent experiments with three replicates per treatment. The data were analyzed using one-way analysis of variance (ANOVA; P < 0.05), and if the F test was significant, the differences were further elucidated through Duncan’s multiple range test. Different letters indicate significant differences at P <0.05. We obtained linear regressions where x is the independent variable (temperature) and y is the dependent variable (fungal growth or antifungal activity of I. javanica pf185). We calculated the Spearman correlation coefficients (R2-values) and its corresponding P -values
표 Effects of various carbon sources and nitrogen sources on the growth of Isaria javanica pf185. The studies were performed in solid and liquid medium with 0.3% yeast extract as the sole nitrogen source or 3% glucose as the sole carbon source and different carbon or nitrogen sources. Data are the means ± standard deviations from two independent experiments with three replicates per treatment, and the different letters indicate significant differences between the fungal isolate pf185 grown using various carbon sources according to one-way analysis of variance (ANOVA) at P < 0.05
표 Effects of various carbon or nitrogen sources on the antifungal activity of Isaria javanica pf185 on solid plates and in liquid medium. Data are the means ± standard deviations from two independent experiments with three replicates per treatment. Data were analyzed using one-way analysis of variance (ANOVA, P < 0.05), and if the F test was significant, the differences were further elucidated through Duncan’s multiple range test. Different letters indicate a significant difference at P < 0.05
표 Effects of the carbon to nitrogen (C:N) ratio on the fungal growth and antifungal activity of Isaria javanica pf185 on solid plates and in liquid medium. The conidial production and antifungal activity of I. javanica pf185 were assessed after 7 days of incubation in liquid media and on solid agar plates containing various C:N ratios with glucose as the sole carbon source and yeast extract as the nitrogen source using a semi-defined liquid medium. Data are the means ± standard deviations from two independent experiments with three replicates per treatment. Data were analyzed through one-way analysis of variance (ANOVA; P < 0.05), and if the F test was significant, the differences were further elucidated through Duncan’s multiple range test. Different letters indicate a significant difference at P <0.05. We obtained linear regressions where x is the independent variable (the C:N ratio) and y is the dependent variable (fungal growth or antifungal activity of I. javanica pf185). We calculated the Spearman correlation coefficients (R2-values) and its corresponding P -values
표 Suppression of disease development of Colletotrichum gloeosporiodes on detached green pepper fruits by treatment of Isaria javanica pf185 conidia. Symptom development was observed at 7 days after pathogen inoculation. (a) Lesion diameter, (b) Reduction rate of lesion diameter. Mean values with different letters indicate statistically significant differences at P ≤ 0.05 when compared by LSD
표 Effect of Isaria javanica pf185 on growth of tobacco (Nicotiana tabaccum ‘Xanthi’) seedlings grown on potting mix. Tobacco seedlings (14-day-old) were treated for five weeks by root drenching with an intact fungal culture (A), cell-free supernatant (B), or spore suspensions (C). Equal volumes of half strength potato dextrose broth or water were used as the control. Means and standard errors of two independent experiments are shown, with six seedlings per replicate. The actual P-values are provided for significant differences in growth under different treatments (Student’s t test, α < 0.05). Insert: images of tobacco seedlings
표 Effect of fungal volatiles on tobacco plant growth. The data shown are of plants two weeks after treatment in I plates. (A) Images of tobacco seedlings and tobacco root hair formation after co-inoculation with spore suspension of Isaria javanica pf185. Images of root hair formation were captured under a microscope. (B) Mean growth parameters of tobacco cultivated with or without I. javanica pf185 (potato dextrose broth) in I plates. A significant difference in responses between treatments and controls, as assessed by Student’s t test (α < 0.01), are indicated with three asterisks. Means and standard errors of two independent experiments are shown, with six seedlings per replicate
표 GC/MS analysis of the volatiles produced by Isaria javanica pf185 grown on potato dextrose agar
표 GC profile of volatiles produced by the fungus Isaria javanica pf185 grown on potato dextrose agar (PDA). The volatiles were absorbed onto a solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrophotometry (GC-MS). GC-MS profiles of volatiles from a non-inoculated PDA bottle (A) and from I. javanica pf185-inoculated PDA bottle. The peaks unique to the volatiles detected in the I. javanica pf185-inoculated PDA bottle are labeled 1-4. These GC-MS profiles are representative of two independent experiments
표 Dibutyl succinate에 의한 I. javanica pf185의 포자 발아율
표 Dibutyl succiante의 점박이응애와 담배가루이 살충율
표 유기농자재와의 혼용에 의한 I. javanica pf185의 균사 생육 억제율
표 Peach aphid efficacy of commercial and liquid prototype of I. javanica pf185. The prototype was diluted 250 times with sterilized water (control) and sprayed on the peach aphids grown in Chinese cabbage. After 24 hours, the rate of live aphid was corrected by abbot method. The same experiment was repeated three times
표 생약시료 320성분에 대한 탄저병 포자 발아 억제율 조사
표 생약시료 320성분에 대한 탄저병 포자 발아 억제율 조사
표 생약시료 320성분에 대한 탄저병 포자 발아 억제율 조사
표 생약시료 320성분에 대한 탄저병 포자 발아 억제율 조사
표 선발된 9가지 추출물에 대하여 탄저병 포자 발아 억제 및 병반면적과 방제가 진딧물에 대한 살충효과 조사
표 최종 후보 물질에 대한 농도별 병방제 효과
표 최종 후보 물질에 대한 농도별 살충 효과
표 Isaria 포자에 대한 고삼 추출물의 유해성 평가
표 Isaria 포자에 대한 대황 추출물의 유해성 평가
표 Isaria 포자에 대한 천연 계면활성제의 유해성 평가
표 Isaria를 배양하기 위한 최적의 배지 조건 확립
표 Isaria를 이용한 시제품 제작 매뉴얼 작성
표 Isaria를 이용한 시제품의 희석 배율 결정
표 Isaria를 이용한 시제품의 보관기간별 포자수 변화
표 1차 액상 시제품의 비해 조사
표 Isaria를 이용한 시제품의 농가 실증 시험
표 Isaria를 이용한 시제품의 병방제 효과
표 Isaria를 이용한 시제품의 병방제 효과
표 분상 제형을 만들기 위한 공정도
표 최종 분상 시제품의 성분 함유량
표 분상 제형의 최종 사용량 결정
표 분상 제형의 항균 활성 기내 실험
표 분상 제형의 실제 포장내 항균 활성 실험
표 분상 제형의 실제 포장내 방제가 조사
표 최종 시제품의 항해충 및 항균활성 기내실험

과제명(ProjectTitle) :	-
연구책임자(Manager) :	-
과제기간(DetailSeriesProject) :	-
총연구비 (DetailSeriesProject) :	-
키워드(keyword) :	-
과제수행기간(LeadAgency) :	-
연구목표(Goal) :	-
연구내용(Abstract) :	-
기대효과(Effect) :	-

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 제목(한글), 저자명(한글), 발행일자, 전자원문, 초록(한글), 초록(영문) 관리번호, 제목(한글), 제목(영문), 저자명(한글), 저자명(영문), 주관연구기관(한글), 주관연구기관(영문), 발행일자, 총페이지수, 주관부처명, 과제시작일, 보고서번호, 과제종료일, 주제분류, 키워드(한글), 전자원문, 키워드(영문), 입수제어번호, 초록(한글), 초록(영문), 목차
저장형식	Text(ASCII format) Excel format
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

고추 병해충(진딧물, 탄저병) 동시방제 미생물제 개발
Development of microbial pesticide for simultaneous control of both aphid and fungal disease of pepper 원문보기

초록

Abstract ▼

목차 Contents

표/그림 (85)

표/그림 (85)

연구자의 다른 보고서 :

참고문헌 (25)

연구과제 타임라인

관련 콘텐츠

원문 보기

이 보고서와 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

고추 병해충(진딧물, 탄저병) 동시방제 미생물제 개발 Development of microbial pesticide for simultaneous control of both aphid and fungal disease of pepper 원문보기

초록

Abstract ▼

목차 Contents

표/그림 (85) 모든 표/그림 보기

표/그림 (85) 슬라이드로 보기

연구자의 다른 보고서 :

한지희 (12) 이상엽 (17) 김다연 (4) 안성호 (3)

참고문헌 (25)

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

이 보고서와 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

고추 병해충(진딧물, 탄저병) 동시방제 미생물제 개발
Development of microbial pesticide for simultaneous control of both aphid and fungal disease of pepper 원문보기

표/그림 (85)

표/그림 (85)