초록 ▼

- 갯지렁이 항균펩타이드 유도제 및 유도조건 개발
- M. luteus P.G 자극 갯지렁이로부터 항염증, 항산화, 항균능 물질 발견
- 발견된 유용물질의 항균능, 항염증, 항산화, 안전성 및 안정성 확인
- M. luteus P.G 자극 갯지렁이로부터 다수의 항균펩타이드 시퀀스 확보
- 갯지렁이 항균펩타이드를 아미노산 서열에 기초하여 합성
- 합성펩타이드의 항균능, 항염증, 항산화, 안전성 및 안정성 확인
- 기존 항균펩타이드와의 경제성 비교 분석
- 국내 주요 양식어종 4종(넙치, 조피볼락,

- 갯지렁이 항균펩타이드 유도제 및 유도조건 개발
- M. luteus P.G 자극 갯지렁이로부터 항염증, 항산화, 항균능 물질 발견
- 발견된 유용물질의 항균능, 항염증, 항산화, 안전성 및 안정성 확인
- M. luteus P.G 자극 갯지렁이로부터 다수의 항균펩타이드 시퀀스 확보
- 갯지렁이 항균펩타이드를 아미노산 서열에 기초하여 합성
- 합성펩타이드의 항균능, 항염증, 항산화, 안전성 및 안정성 확인
- 기존 항균펩타이드와의 경제성 비교 분석
- 국내 주요 양식어종 4종(넙치, 조피볼락, 흰다리새우, 뱀장어)의 실험실 규모 8주간 사육실험
- 국내 주요 양식어종 3종(넙치, 조피볼락, 흰다리새우)의 양식장 규모 16주간 사육실험
- 사육실험 후 대상어종의 면역능 개선 및 어병세균 인위감염에 대한 항병성 검증
- 갯지렁이 항균펩타이드 분말이 첨가된 양어용 사료의 경제성 분석
- SCI급 논문 6건, 비SCI급 논문 3건 개제
- 특허출원 4건, 특허등록 2건
- 시제품 개발 2건, 기술이전 2건
- 학사 2명, 석사 3명, 박사 4명의 인력양성

(출처 : 요약서 3p)

Abstract ▼

Purpose & Contents
1. Purpose : Development of functional antimicrobial peptide for food additives derived from marine worms to prevent the diseases and enhance immunity of farming marine creatures
- Separation of effective and index components, purification and characterization of anti-oxidan

Purpose & Contents
1. Purpose : Development of functional antimicrobial peptide for food additives derived from marine worms to prevent the diseases and enhance immunity of farming marine creatures
- Separation of effective and index components, purification and characterization of anti-oxidant and anti-inflammatory components, discovery of material with anti-oxidant, anti-inflammation and enhancement of immune function.
- Evaluation of dietary inclusion of the lugworm-originated functional peptide on fish performance in the lab scale and challenging test
- Assay of anti-microbial activity to fish diseases, comparison analysis of potency and economic feasibility with the existing antibiotics, stability verification, verification of stability and efficacy of peptide in EP production, development of mass production process.
- Design and manufacturing of control feed for main fishes (olive flounder, black rockfish, shrimp, etc), breeding test and verification of efficacy, safety verification in feeding food additives.

2. Contents
○ Discovery of functional material from marine worms for food additives
- Optimization to develop immune enhancer
- Separation of effective and index components
- Purification and structure identification
- Discovery of material for food additives with anti-oxidation, anti-inflammation and immune enhancement
○ Development of antimicrobial peptide from lu worms
- Separation and structure analysis of antimicrobial peptide
- Assay of antimicrobial activity to bacteria causing fish diseases
- Verification of Safety and stability of antimicrobial peptide
- Development of natural antibiotics using lug worm powder
○ Development of manufacturing process for antimicrobial peptide
- Gene screening for antimicrobial peptide from lug worm
- Development for recombinant antimicrobial peptide
- Development for synthetic antimicrobial peptide
- Verification of safety and stability for antimicrobial peptide
- Comparison analysis of efficacy and economic feasibility with the existing antibiotics
- Verification of safety and stability for antimicrobial peptide in EP manufacturing
○ In vivo experiment
- Development manufacturing antimicrobial peptide added feed for olive flounder in the lab scale and farm scale olive flounder feeding test
- Development manufacturing antimicrobial peptide added feed for black rockfish in the lab scale and farm scale olive flounder feeding test
- Development manufacturing antimicrobial peptide added feed for white leg shrimp in the lab scale and farm scale olive flounder feeding test
- Lab scale Japanese eel feeding test
○ Development of production
- Technology transfer and production of experimental product

Results
- Antimicrobial peptide from marine worm can reduce the pollution of the marine environment, the disturbance of ecosystem, and the occurrence of resistance bacteria
- Antimicrobial peptide will lower the production cost
- Diversity of food additives derived from marine animal.
- Increasement of fisherman's income by invigoration of lug warm farming.

Expected Contribution
- Antimicrobial peptide from marine worm is applicable not only to farming fishes but also to livestocks whose industry is far larger than fish industry
- Antimicrobial peptide can export abroad because the diseases of farming fishes or animals are same worldwide
- Reduction of the occurrence of resistance bacteria and lowering of the production cost by less usage of medicines.
- Establishment of world-class competitiveness for supplying safe fishes and livestocks by using eco-friendly food additives (Promotion of export).
- Promotion of fish industry and enhancement of added value by developing highly functional food additives
- Product differentiation from imported farming fishes from China and south east Asia.
- Value creation of novel fusion industry to develop new materials from marine product.
- Application of antibiotic substituent to diverse field (Fishery, agriculture, food, environment, and medicines).
- Contribution to Creative economy and globalization of food additives derived from marine product as new field.

(출처 : SUMMARY 6p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문요약문 ... 4
• SUMMARY ... 6
• CONTENTS ... 8
• 목차 ... 9
• 제1장. 연구개발과제의 개요 ... 10
• 제2장. 국내외 기술 개발 현황 ... 16
• 제3장. 연구 수행 내용 및 성과 ... 20
• 1. 1차년도(2015년) ... 20
• 2. 2차년도(2016년) ... 49
• 3. 3차년도(2017년) ... 107
• 제4장. 목표 달성도 및 관련 분야 기여도 ... 144
• 제5장. 연구개발성과의 활용계획 ... 146
• 제6장. 연구 과정에서 수집한 해외 과학기술 정보 ... 148
• 제7장. 연구개발성과의 보안등급 ... 149
• 제8장. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 150
• 제9장. 연구개발과제 수행에 따른 연구실 등의 안전 조치 이행 실적 ... 151
• 제10장. 연구개발과제의 대표적 연구 실적 ... 153
• 제11장. 기타 사항 ... 156
• 제12장. 참고 문헌 ... 157
• 끝페이지 ... 160