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• 그림 Pedigree of ‘Sinhwa’ pear
• 표 Tree characteristics of ‘Sinhwa’ in Naju from 2007 to 2009
• 그림 Fruit (left) bagging with paper bag at harvest and flower cluster (right) of ‘Sinhwa’ pear
• 표 Flower characteristics of ‘Sinhwa’ in Naju from 2007 to 2009
• 표 Leaf characteristics of ‘Sinhwa’ in Naju from 2007 to 2009
• 표 Fruit characteristics of ‘Sinhwa’ in Naju from 2007 to 2009
• 표 Cross compatibility of ‘Sinhwa’ (2012)
• 그림 S-genotypes analysis of Shinhwa(S5S?) cultivars by PCR-RFLP analysis. L,1Kb ladder; 1,Uncut PCR product; 2,SfcⅠ(S1 specific); 3,AflⅡ(S2 specific); 4,PpuMI (S3,S5 specific); 5,AlwNI (S5 specific); 6,NdeⅠ(S4 specific); 7,HinCⅡ(S6, S7 specific); 8,MluⅠ(S7 specific); 9,NruⅠ(S8 specific); 10,BstBⅠ(S9 specific). Each digested fragment was loaded using the electrophoresis system
• 그림 Multiple sequence alignment among cDNA of ‘Shinhwa’, S5_mRNA (GeneBank accession number D88282) from P. serotina and S5_gDNA (GeneBank accession number AB045711) from ‘Chojuro’. Asterisks (*) denoted coincidence with nucleotide of S5_mRNA and S5_gDNA, under-lined sequences denote intron of the S5 sequence
• 그림 Germination and growth of pollen tubes in the squashed styles of ‘Whangkeumbae’ × ‘Shinhwa’, Some pollen tube tips were elongated continuously, but others hypertrophy and stopped
• 표 Tree characteristics of ‘Seolwon’ in Naju from 2008 to 2010
• 표 Fruit characteristics of ‘Seolwon’ in Naju from 2008 to 2010
• 그림 Fruit (left) bagging with paper bag at harvest and flower cluster (right) of ‘Seolwon’ pear
• 그림 Fruit flesh color change in room temperature condition (Left, after right cutting, ; Middle, 1 day after cutting; Right, 2 days after cutting)
• 표 Evaluation of Seolwon fresh-cut characteristics (2010, Naju)
• 그림 Flesh color change of ‘Seolwon’ at 4℃ ※ Manufactured date Sep. 30, They were preserved at 4℃ condition
• 표 Evaluation of 6 cultivars fresh-cut characteristics (2010, Naju)
• 그림 Flesh browning of new cultivars after fresh cut manufacturing zFiber of flesh have been browning after fresh cut manufacturing at room temperature
• 그림 S-genotypes analysis of Seolwon(S3S9) cultivars by PCR-RFLP analysis. L,1Kb ladder; 1,Uncut PCR product; 2,SfcⅠ(S1 specific); 3,AflⅡ(S2 specific); 4,PpuMI (S3,S5 specific); 5,AlwNI (S5 specific); 6,NdeⅠ(S4 specific); 7,HinCⅡ(S6, S7 specific); 8,MluⅠ(S7 specific); 9,NruⅠ(S8 specific); 10,BstBⅠ(S9 specific). Each digested fragment was loaded using the electrophoresis system
• 그림 Germination and growth of pollen tubes in the squashed styles of ‘Whangkeumbae’ × ‘Supergold’ (A) ‘Whangkeumbae’ × ‘Shinhwa’ (B) ‘Niitaka’ × ‘Seolwon’ (C). (A) and (C): All pollen tube tips elongated were hypertrophied and stopped. (B): Some pollen tube tips were elongated continuously, but others hypertrophy and stopped
• 표 Tree characteristics of ‘Joyskin’ in Naju from 2009 to 2011
• 표 One year shoot characteristics of ‘Joyskin’ at Naju in 2011
• 표 Flower characteristics of ‘Joyskin’ at Naju from 2009 to 2011
• 표 Fruit characteristics of ‘Joyskin’ at Naju from 2009 to 2011
• 표 Cross-compatibility of ‘Joyskin’ with other known cultivars
• 그림 Germination and growth of pollen tubes in the squashed styles of ‘Joyskin’ × ‘Shinseiki (S3S4)’(A), ‘Joyskin’ × ‘Imamuraaki(S1S6), (B). A, B; Selfincompatibility allele of pistill and pollen cultivar partially coincided each other. So, pollen tube tip was hypertrophy and also elongation stopped (red circles) and the others elongated continuously
• 그림 PCR-RFLP results for confirmation of the ‘Joyskin’ self-incompatibile gene with S-allele specific primer (Forward; FTQQYQ, Reverse; anti-TIWPNV(ACG TTT GGC CAA ATA GTT)). Uncut PCR product (1) and results digested by endonucleases (2-6). 380bp PCR product (red dot) was digested in 120, 260bp (red arrow) by Only PpuMI and 360bp PCR product (blue dot) was digested in 240 and 12bp by MluⅠ and 230 and 130bp by HincⅡ. M, 1Kb ladder; 1, Uncut PCR product; 2, PpuMI (S3,S5specific);3,AlwNI (S3,S5specific);4, MluⅠ (S7specific);5,HincⅡ (S6,S7specific);6,NdeⅠ (S4specific).Eachdigestedfragmentwasloadedusingtheelectrophoresissystem
• 그림 Confirming of stone cells by 3% phloroglucinol-HCl dye in fresh ‘Joyskin (A)’, Niitaka (B)’, ‘Manpungbae (C)’, ‘Whangkeumbae (D)’, pear using microscope (40×) on harvest time
• 그림 Pedigree diagram of ‘Greensis’ pear (A) and shape of fruit and flower (B)
• 표 Pear scab incidence of the six pear cultivars by artificial inoculation
• 표 Tree characteristics and disease resistance of ‘Greensis’ in Naju from 2009 to 2012
• 표 Fruit characteristics of ‘Greensis’ at Naju from 2009 to 2012
• 표 Flower characteristics of ‘Greensis’ at Naju from 2009 to 2012
• 표 Leaf characteristics of ‘Greensis’ at Naju from 2009 to 2012
• 그림 Sequence alignment of 380 bp and 1,000 bp PCR products of ‘Greensis’. A, 380 bp PCR product and S4 allele of ‘Nijisseiki’ complete CDs (GenBank:AB009385.1); B, 1,000bp PCR product and Seallele of Williams (GenBank:DQ871234), Beurre Precoce Morettini (DQ871231), Rocha (DQ663567), and Clapp’s Favorite (DQ871227) partial CDs in Pyrus communis
• 표 Tree characteristics and disease resistance of ‘Sodam’ in Naju from 2011 to 2013
• 표 Fruit characteristics of ‘Sodam’ at Naju from 2011 to 2013
• 표 Flower characteristics of ‘Sodam’ at Naju from 2011 to 2013
• 표 Fruit characteristics change of Sodam in low temperature(1℃) and change temperature condition (Naju, 2018)
• 표 Tree characteristics and disease resistance of ‘Sowon’ in Naju from 2011 to 2014
• 표 Fruit characteristics of ‘Sowon’ at Naju from 2011 to 2014
• 표 Flower characteristics of ‘Sowon’ at Naju from 2011 to 2014
• 그림 PCR-RFLP results for confirmation of the ‘Sowon’ self-incompatibile gene with S-allele specific primer (Forward; FTQQYQ, Reverse; anti-TIWPNV(ACG TTT GGC CAA ATA GTT)). 1, Chuhwangbae uncut; 2, Chuhwangbae/PpuM Ⅰ (It means Chuhwangbae PCR product was digested by Ppu MⅠ); 3, Chuhwangbae/Alw NⅠ; 4, Chuhwangbae/Nde Ⅰ; 5,Chuhwangbae/Mlu Ⅰ; 6, Hosui uncut; 7, Hosui/Ppu MⅠ; 8, Hosui/Alw NⅠ; 9, Hosui/Nde1; 10, Hosui/Mlu Ⅰ; 11, Sowon uncut; 12, Sowon/Ppu MⅠ(Red box showed digested products in 120, 260bp by Ppu MⅠ); 13, Sowon/Alw NⅠ(No digestion); 14, Sowon/Nde Ⅰ(Red box showed digested products in 140, 240bp by Nde Ⅰ); 15, Sowon/Mlu Ⅰ
• 표 Tree characteristics and disease resistance of ‘Aramchan’ in Naju from 2009 to 2014
• 표 Fruit characteristics of ‘Aramchan’ at Naju from 2009 to 2014
• 표 Flower characteristics of ‘Aramchan’ at Naju from 2009 to 2014
• 그림 PCR-RFLP results for confirmation of the ‘Sowon’ self-incompatibile gene with S-allele specific primer (Forward; FTQQYQ, Reverse; anti-TIWPNV(ACG TTT GGC CAA ATA GTT)). A: Hwangkeumbae (S3S4) B: Whasan (S5,S?), C: Aramchan (S3,S?), M: 1Kb ladder, 1: uncut, 2: sfc 1, 3. Afl 2, 4: PpuM 1, 5: AlwN1, 6: Nde 1, 7: HinC 2 8: Mlu 1, 9: Nru 1, 10: BstB 1. (2∼10: digested by endonucleases)
• 표 Tree characteristics and disease resistance of ‘Yescool’ in Naju from 2013 to 2015
• 표 Fruit characteristics of ‘Yescool’ in Naju from 2014 to 2016
• 그림 Box-and-whisker diagram of Yescool and Supergold fruit weight in 2017-2018
• 표 Flower characteristics of ‘Yescool’ at Naju in 2016
• 표 Results of shelf life test of ‘Yescool’ under room temperature (2016)
• 표 Tree characteristics and disease resistance of ‘Censall’ in Naju from 2015 to 2017
• 표 Fruit characteristics of ‘Censall’ at Naju from 2015 to 2017
• 그림 Anatomical observation of Censall skin structure on optical microscope (A, Immatured Censall, left, ×200, right, ×400; B, matured fruit, left, ×200, right, ×400; C, Niitaka) C, cork layer; H, hyperdermis; IS, intercellular space, OE, outer epidermis; SC, stone cell; SG, starch granule
• 표 Anatomical structure of Censall fruit skin
• 표 Flower characteristics of ‘Censall’ in Naju (2017)
• 그림 Comparison of seed development between Hwangkeumbae (Left) and Censall (Right) in 22. July 2014
• 그림 Comparison of seed development over time (Left: Seed development status in 30days after full bloom; Right: Seed development status in 50 days after full bloom)
• 그림 Result of low cytometry analysis of Censall (Left) and Manpungbae (Right)
• 그림 배 설렘 품종의 육성 계보도
• 그림 배 ‘설렘’ 품종의 꽃눈, 꽃 및 과실 모습
• 표 배 설렘 품종의 수체특성 및 병 발생 특성 (2015∼2019년, 나주)
• 표 배 설렘 품종의 과실특성 (2015∼2019년, 나주)
• 그림 배 품종별 착즙 후 경과 시간에 따른 갈변 모습
• 그림 ‘설렘(원교나-72호)’ 주스 색택(좌: 420nm, 우: 660nm)
• 그림 배 설렘 품종의 과육당 과즙량 비교(ml/200g)
• 그림 배 진향 품종의 육성 계통도
• 표 배 진향 품종의 수체특성 및 병 발생 특성(2015∼2019년, 나주)
• 표 배 진향 품종의 과실특성 (2015∼2019년, 나주)
• 그림 배 진향 품종의 향기성분 분석표
• 그림 배 진향 품종을 이용한 맥주 시제품 관능 평가 결과
• 그림 Promotion and new cultivar pannal test (2018)
• 표 High soluble solids content elite line in Shinseiki × Chuhwangbae seedlings (2016)
• 표 High soluble solids content elite line in Shinseiki × Chuhwangbae seedlings (2017)
• 그림 Elite lines Seolwon × Chuhwangbae seedlings in 2017
• 표 High soluble solids content elite line in Seolwon × Chuhwangbae seedlings (2018)
• 그림 Sugar contents distribution of Seolwon × Chuhwangbae seedlings (n=50, 2018)
• 표 Main characteristics of red skin pear seedlings (2018)
• 그림 Expression red skin color of Mantienhong × Supergold seedlings
• 그림 Red skin pear elite line 3-8-40 (Supergold × Mantienhong, 2012 crossed)
• 그림 Red skin pear elite line 5-23-203 (Supergold × Canal pear, 2011 crossed)
• 그림 Red skin color charcteristics expression of Supergold × Kalle seedlings
• 그림 Seeds development of Wonhwang × Osanijiseiki seedlings fruit
• 표 Inherent characteristics of self compatibility in Wonhwang × Osanijiseiki seedlings (2016)
• 표 Fruit characteristics and fruit set ratio of Wonhwang × Osanijiseiki (2016, 2018)
• 그림 Summary of pear breeding history in Korea
• 그림 Good pollen careful selection process using acetone
• 그림 Preparation materials for artificial pollination (left), removing the stamens (middle), artificial pollination (right)
• 그림 Microscopic observation of pollen shape A,Pyrus sp.; B,Brassica napus C,P inus densiflora; D, Schlum bergera; E,Malus sp. (C, D, E were quoted by http://yonggulee.egloos.com/)
• 그림 Microscopic observation of mixed pollen between Pyrus sp. and Brassica napus. A, dried condition; B, hydrolic condition; (magnification 100×)
• 그림 Climatic conditions of temperature, wind, rainfall during April, 2018∼2017. Full blooming day, 2017: 10, April ~ 15, April; 2018: 3. April ~ 8. April)
• 그림 High speed wind treatment for simulation
• 그림 Microscopic observation of attached pollen and stigma condition by strong wind treatment (18~20m/sec) A, Stigma of full bloom (No pollination); B, Right after Pollination; C, After high speed wind treatment flower against
• 그림 Harmed Manpungbae fruit by damaged paper bag(A, by bird; B, fruit moth)
• 표 Damaged paper bag frequency and fruit weight of Manpungbae and Mansoo
• 표 Main characteristics and preference of juice by cultivars
• 그림 Change pear juice color by extracted part ※1, Niitaka; 2, Hwangkeumbae; 3, Seolwon; 4, Chuhwangbae; 5, Wonhwang; 6, Manpungbae; 7, Manhwang
• 표 Chromaticity of pear juice extracted from whole fruit (A) and flesh (B) each cultivar
• 표 Browning and turbidity of pear juice from whole fruit (A) and flesh (B) each cultivar
• 표 Fruit characteristics of each pear cultivar for freeze drying process (2012)
• 표 Comparison of dry freezed flesh texture by cultivar (2012)
• 그림 Results of hierarchical cluster analysis among main pear cultivars in freezing dried fruit flesh. (1, Manpungbae; 2, Whangkeumbae; 3, Wonhwang; 4, Seolwon; 5, Manhwang; 6, Hanareum; 7,Chuwhangbae; 8, Niitaka.) Clustering: Euclidian distance, ward method. (2012) Cluster Ⅰ : Hardness and chewiness is very low and soft Cluster Ⅱ : Normal Cluster Ⅲ : Springness is very high and hardness is normal Cluster Ⅳ : Hardness is high and it is easy to break for high chewiness and springness
• 표 Cross compatibility of ‘Supergold’ (2012)
• 그림 Germination and growth of pollen tubes in the squashed styles of ‘Supergold’ × ‘Okusankichi (S5S7), (A); ‘Supergold’ × ‘Chojuro (S2S3), (B); ‘Supergold’ בYakumo (S1S4), (C); ‘Supergold’ × ‘Nijisseiki (S2S4), (D); ‘Whangkeumbae (S3S4)’ × ‘Supergold’. (E); B, C, D, Selfing compatibility allele of pistill and pollen cultivar partially coincided each other. So, pollen tube tip was hypertrophy and also elongation stopped (red circles) and the others elongated continuously; (E), All pollen tube tips elongated were hypertrophied and stopped
• 그림 S-genotypes analysis of Supergold by PCR-RFLP analysis. L,1Kb ladder; 1,Uncut PCR product; 2,SfcⅠ (S1 specific); 3,AflⅡ (S2 specific); 4,PpuMI (S3,S5 specific); 5,AlwNI (S5 specific); 6,NdeⅠ (S4 specific); 7,HinCⅡ (S6, S7 specific); 8,MluⅠ (S7 specific); 9,Nru Ⅰ (S8 specific); 10,BstBⅠ(S9 specific). Each digested fragment was loaded using the electrophoresis system
• 그림 Microscopic observation of stone cell distribution (40×)
• 표 Firmness (N) of pear skin and flesh by cylinder and blade type plunger of texture analyzer for selection standard
• 그림 Microscopic observation of stone cell distribution in pear skin (40×)
• 표 Materials used in fruit characteristics analyze during fruit development
• 표 Pearson correlations coefficients between leaves and fruit characteristics in 2012
• 그림 Corelation with total soluble solids content and among monosacarides (A, Sucrose; B, Glucose; C, Fructose; D, sorbitol, 2015)
• 그림 Soluble solids content distribution among seedlings in 2012∼2019 (Hwangkeumbae × Chuhwangbae, n=121; Hwangkeumbae × Minibae, n=153, Manpungbae × Ooharabenni, n=205, Hanareum × Ooharabenni, n=76, )
• 표 Change of soluble sugar composition and fruit characteristics during fruit development in Pyrus bretschneideri pears in 2015
• 표 Change of soluble sugar composition and fruit characteristics during fruit development in P. communis pears in 2014
• 표 Change of soluble sugar composition and fruit characteristics during fruit development in P. pyrifolia pears in 2015
• 표 Change of soluble sugar composition and fruit characteristics during fruit development in P. pyrifolia × P. communis pears
• 표 Change of soluble sugar composition and fruit characteristics during fruit development in P. pyrifolia × P. communis pears. (Continue)
• 표 Pearson correlations coefficients among fruit characteristics
• 표 Change of fruit characteristics during fruit development of ‘Hanareum’ pear (P . pyrifolia Nakai) in 2014
• 그림 Change of flesh color after iodine test during fruit development of ‘Hanareum’ pear in 2014. A, 82 days after full bloom (DAFB); B, 96 DAFB; C, 110 DAFB; D, 115 DAFB; E, 119 DAFB; F, 125 DAFB; G, 130 DAFB; H, 135 DAFB
• 표 Change of soluble sugar composition during fruit development in ‘Hanareum’ pears in 2014
• 그림 The scheme of ripening time decision based on the soluble sugar composition and fruit physical characteristics by fruit development period. zDAFB, days after full bloom. yEach graded gray color means grouped fruit characteristics by fruit development status based on statistic significance. xYellow square means physiological ripening time
• 표 Pearson correlations coefficients among main physicochemical characteristics of Hanareum
• 그림 Concentration of Genomic DNA using electrophoresis
• 표 Condition of Partially digestion reaction buffer components
• 그림 Partially digestion using Hind III and EcoR I endonuclease. Lane 1 and 11, λ PFG marker; 12, 1Kb ladder, 3, Hind III 0.05U digested DNA; 4, Hind III 0.15U digested DNA; 5, Hind III 0.3U digested DNA; 6, Hind III 0.6U digested DNA; 7, EcoR I 0.05U digested DNA; 8, EcoR I 0.15U digested DNA; 9, EcoR I 0.3U digested DNA; 10, EcoR I 0.6U digested DNA. 1% PFG gel, 200V, 0.1~0.4sec, 14hr run
• 그림 Size-frcation of partially digest using H indⅢ and EcoRI endonuclease. (※ Hind III and EcoR I endonuclease 모두 0.05U 조건으로 4㎍ 씩 추가 digestion 한 후 전 량(12㎍)을 모두 Run 해서 elution 함)
• 표 Condition of End repair enzyme mixture
• 표 Condition of ligation mixture
• 표 Preparation of NotI endonuclease mixture
• 그림 Distribution of randomly fosmid clone. The NotI digested DNA was separated via pulsed field gel electrophoresis using DNA makrer loaded on both side
• 그림 High quality clone library DNA preparation
• 그림 Verification of clone map library purification
• 표 Pre-made of end sequencing mixture
• 그림 Condition of Sequencing PCR
• 그림 Methods of end sequencing using 3730XL
• 그림 Processing of Phredphrap
• 표 Results of fosmid library construction
• 표 Results of fosmid pair-end sequencing
• 그림 Distribution of fosmid end sequencing results
• 표 Results review for comparison relationship between sugar contents and SSR marker candidates originated from Hawngkeumbae
• 표 Results review for comparison relationship between sugar contents and SSR marker candidates originated from Hawngkeumbae Continue
• 표 Diversity statistics for 15 SSR loci studied in 49 pear cultivars
• 표 Diversity statistics for 15 SSR loci studied in 49 pear cultivars continued
• 그림 Dendrogram depicting the classifications of 49 pear cultivars and germplasm samples constructed using the unweighted pair-group method with arithmetical average (UPGMA) and based on microsatellite markers
• 그림 Pear genome analysis plan
• 표 Information of reference genome
• 그림 Figure of Manpungbae×Oharabeni seedlings
• 표 Statistics value of GBS raw data
• 표 Raw data statistics value by demultiplexing
• 표 Raw data statistics value by demultiplexing continue
• 표 Number of candidate SNP markers for genetic mapping
• 그림 Genetic inkage map of Manpungbae and Oharabeni seedlings
• 표 SNP markers statistics for genetic linkage map
• 표 SNP markers statistics for genetic linkage map
• 표 Significant QTL positions for acidity, fructose, glucose, sorbitol
• 표 Significant QTL positions for acidity, fructose, glucose, sorbitol Continued
• 표 Significant QTL positions for acidity, fructose, glucose, sorbitol Continued
• 표 Significant QTL positions for acidity, fructose, glucose, sorbitol Continued
• 표 Cross compatibility of ‘Manpungbae’ and ‘Wonhwang’
• 그림 Crossed section fruit of Wonhwang × Manpungbae (A) and immatured seeds (B)
• 그림 Pollen tube growth in the squashed styles of ‘Manpungbae’ pear cultivar Cross-pollinated with ‘Wonhwang’
• 표 Results of RT PCR product
• 그림 Phylogenetic tree based on sequencing results
• 표 Male sterility in pear seedlings
• 그림 Monitering by microscpy of pollen developmentv stage in development stage in pear cultivars(1,000x). A, B, Development of archesporial tissue(AS); C, Formation of microspore mother cells(MMC); D, Meiosis stage(Meiosis); E, Formation of microspore tetrad(Tetrad); F, Tetrad and pollen formation stage; G, Development of microspores(DM); H, Abnormal shape of pollen; I, Pollen formation(PF); J, Normal and abnormal pollens; K, Synthesis of starch in pollen grains(SP); L, Normal and abnormal pollens
• 표 Seedlings for candidated marker selection
• 표 내건 내습성 대목 집단 선발을 위한 시험수 양성 교배
• 그림 PEG와 NaCl 농도 처리에 따른 수체 반응 A : PEG 처리 MS, 5, 10, 15, 20, 25% (왼쪽부터) B : NaCl 처리 MS, 1, 2, 3% (왼쪽부터)
• 그림 PEG 처리에 의한 수분스트레스 유발에 따른 품종별 생육 차이
• 그림 NaCl 농도 변화에 따른 배 품종의 Proline 함량 변화
• 그림 NaCl 처리 농도에 따른 품종별 생육상황
• 그림 PEG 처리와 수분 스트레스 관계
• 그림 배 품종별 PEG 농도 처리에 의한 식물체 생체중과 프롤린 함량
• 그림 배 품종별 PEG 농도 처리에 의한 식물체 생육상황
• 그림 PEG(25%) 하에서 만황 기내접목, 계대배양 4주 후 A: 만황/OPR195 OP, B:만황/PBPC
• 표 PBPC, OPR 195 op에 기내접목한 만황 품종의 생육특성
• 그림 대목별 기내접목된 접수품종 만황의 프롤린함량
• 그림 건조처리 후 대목 교배집단별 반응
• 그림 경지삽 처리 모습
• 그림 대목의 발근 처리 결과
• 그림 왜성대목계통 생장상태
• 표 호르몬 농도 처리에 따른 품종 별 발근율
• 그림 PEG 처리 1달 후 만풍배 실생 생육상태
• 그림 PEG처리 유무에 따름 뿌리 끝 상태와 현미경 관찰
• 그림 OPR195×OPR249 집단의 PEG 처리 결과
• 그림 PEG 처리 농도에 따른 대목의 활력차이
• 그림 PEG처리에 따른 OPR195xOPR249 실생과 콩배 실생의 생육 반응
• 표 목표형질에 따른 신규 교배집단 구성 및 양성 현황
• 표 기내 조직배양 묘 PEG 처리농도별 생장 반응
• 그림 PEG 처리 7일 후 각 농도별 대목의 피해 모습
• 표 배연 3호 접목묘 생육상황
• 그림 접수 품종 생육상태 집괴분석
• 표 대목 신규교배조합 작성 현황(2013)
• 표 Pyrus calleryana 실생을 대상으로 침수처리 하였을 때 나타난 엽록소와 폴리페놀 함량 변화
• 표 침수처리에 따른 Pyrus calleryana 실생의 유리 아미노산 함량 분석
• 표 침수처리에 따른 Pyrus calleryana 실생의 구조 아미노산 함량 분석
• 표 옥신 함유 MS 배지에서 배양한 추황OP 배양묘의 발근율과 평균 뿌리 길이
• 표 호르몬 액에 순간 침지한 추황OP 배양묘의 발근율과 평균 뿌리 길이
• 그림 다양한 옥신 처리에 의한 추황OP 실생의 뿌리 발생 형태의 차이; 식물체는 다양한 식물 호르몬 액에 순간 침지한 후 MS 배지에서 30일간 배양됨
• 그림 대목 자원들의 과습 스트레스에 의한 생장량(수체높이) 변화량
• 그림 시험처리에 따른 대목 자원들의 엽록소 a 성분량의 변화 Control_Cvt : 관행재배군이며 생육 초기 시험처리 전 Control_Dmp : 습해처리군이며 생육 초기 시험처리 전 Treatment_Cvt : 관행재배군이며 습해를 주지 않고 정상 생육시킴 Treatment_Dmp : 습해처리군이며 습해를 주며 생육시킴
• 그림 시험처리에 따른 대목 자원들의 엽록소 b 성분량의 변화 Control_Cvt : 관행재배군이며 생육 초기 시험처리 전 Control_Dmp : 습해처리군이며 생육 초기 시험처리 전 Treatment_Cvt : 관행재배군이며 습해를 주지 않고 정상 생육시킴 Treatment_Dmp : 습해처리군이며 습해를 주며 생육시킴
• 그림 시험처리에 따른 대목 자원들의 엽록소 a+b 성분량의 변화
• 표 대목 자원별 폴리페놀 함량변화 비교
• 표 옥신 호르몬 순간침지와 MS 배지 농도에 따른 CHOP 조직배양묘의 발근양상
• 그림 왜성대목 계통 CHOP의 실제 크기
• 그림 유망한 배 왜성대목 계통들의 재식 현황
• 그림 봉지종류별 과피색 발현 양상 A, 착색봉지(빛을 차단하여 엽록소 생성 억제); B, 신문봉지; C, 황색롤지; D, 무대 (좌, 무봉지재배; 우, 봉지재배)
• 표 대원홍 괘대 봉지물성 분석
• 그림 적색과피 교배실생의 엽색 변화
• 그림 적색과피 배 교배실생의 시기별 과피색 변화 양상; 수양홍리×대원홍
• 표 적색과피 품종 육성 신규 교배 : ‘Max Red Bartlett’ב만풍배’ 등 8조합
• 그림 배 종간교잡 집단의 가계도, ‘P019R045T042 × Shinko’의 교배실생 집단이 연구에 활용됨
• 그림 Venturia nashicola 표준균주 (NJBW-1214), (좌)균주 stock, (우)
• 그림 YMA, PDA 배지 위에 증식된 NJBW-1214 분리체
• 그림 YMA(좌), PDA(우) 배지 위의 NJBW-1214 균사체 (포자 생성 안됨)
• 그림 지퍼백을 사용한 표현형 검정 방법
• 그림 검은별무늬병 표현형 검정을 위한 기준(Score1∼3:저항성, Score4∼5:이병성)
• 표 실험집단의 검은별무늬병 표현형 스코어 분포
• 그림 Elshire et al.(2011)에 의한 GBS 분자표지 개발 방법
• 그림 증폭된 library의 fragment analysis 결과
• 그림 증폭된 각각의 library의 fragment analysis 결과 및 품질
• 그림 SNP calling raw data
• 표 Called SNP marker의 특성 분류 및 구분
• 그림 Shinko × P019R045T042 의 physical map, 염색체 1, 2, 5, 8번이 누락됨
• 그림 원황의 consensus map
• 표 Mapping에 사용된 마커의 contig group
• 그림 사과의 유전체에서 나타나는 genome duplication chromosome, 배의원종에서도동일하게 적용됨