초록

◾시료 확대 (다소비→지역농상물) 및 생체중량 단위를 포함하는 플라보노이드 DB 개정으로 활용도 증진
◾인체 유래물 (소량의 혈액 및 소변)의 플라보노이드 대사체 분석기술 확립
◾중재연구 및 관찰연구를 통한 발효콩 섭취 바이오마커 발굴

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
Investigation of metabolite profiles changes of traditional fermented products intake by human metabolism process.
Update on characterization of flavonoids in commonly consumed agro-foods and application of previously constructed Flavonoids Database 1.0 (’16, RDA). Identific

□ Purpose&Contents
Investigation of metabolite profiles changes of traditional fermented products intake by human metabolism process.
Update on characterization of flavonoids in commonly consumed agro-foods and application of previously constructed Flavonoids Database 1.0 (’16, RDA). Identification of metabolites in urine and plasma according to intake of fermented diets such as soybean and napa cabbage. Investigate the difference in bioavailability according to fermentation and to discover flavonoid metabolite components as dietary biomarker.

□ Results
Flavonoid content in constructed database was expressed interms of “dry weight” was upgraded by providing the moisture contents of each samples. Moreover, phenolic derivatives fromlocal specialties was included. Phenolic metabolites in human urine and plasma were analyzed (sample volume was 500 uL) and optimized without enzymatic hydrolysis (intact form). For discovery of phenolic biomarker, analysis of ingredients, diets, and human samples was confirmed the effect of fermentation on the metabolite profiles. Isoflavone metabolites conjugated with sulfate and glucuronide were significantly higher in fermented than non-fermented soybean diets. Phenolic conjugates with sulfate and glycine were newly identified in urine after intake of napa cabbage diet. Blood and urine samples were collected through interventional and observational studies to analyze human metabolites. The structural changes in food isoflavones by fermentation showed in faster absorption and higher bioavailability after the intake of FS compared with the infake of the NFS diet. This is the first study to suggest that the isoflavone conjugate profile, rather than total isoflavone levels, might be a more appropriate marker to discriminate the consumption of FS and NFS diets.

□ Expected Contribution
Previous studies for flavonoid metabolites were limited to discuss about bioavailablity based on intake of different diet type. This study provide an information and analytical tools for the assessment of functional foods and their metabolites using LC-MS. The experimental design of this study was based on the common fermented diet in Korean cuisine which can be considers as a functional food due to the presence of diversehealth beneficial metabolites. Hence, this finding can be extended to other food samples for similarly metabolite assessment.
These findings might provide scientific insights into the metabolism and bioavailability of flavonoid, which could ultimately contribute to understand positive effects of flavonoids on human health with further studies in large population-based study taking into account other factors such as host-microbial interactions.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 5
• 목차 ... 7
• 제 1 장 연구 개발 과제의 개요 ... 8
• 제1절 연구 개발 목적 ... 8
• 제2절 연구 개발의 필요성 ... 9
• 제3절 연구 개발 범위 ... 10
• 제 2 장 연구 수행 내용 및 결과 ... 11
• 제1절 농산식품자원별 플라보노이드 식품 대사체 구명 ... 11
• 1. 플라보노이드 데이터베이스 정보 갱신 및 개정 ... 11
• 2. 농식품자원의 플라보노이드 대사체 분석 및 인체 대사체 연구 ... 36
• 제2절 Metabolomics를 활용한 플라보노이드 인체 대사체 구명 ... 79
• 1. 한국인 대표식품(콩/배추)의 인체 대사체 구명을 위한 중재연구 ... 79
• 2. 한국인 식이에 대한 인체 대사체 구명을 위한 관찰연구 ... 103
• 제 3 장 목표달성도 및 관련분야 기여도 ... 111
• 제1절 목표대비 달성도 ... 111
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 112
• 제 4 장 연구 결과의 활용 계획 ... 113
• 제 5 장 연구개발과제의 대표적 연구실적 ... 114
• 제 6 장 기타사항 ... 115
• 제 7 장 참고문헌 ... 116
• 끝페이지 ... 118