초록

꽃양귀비 유전체 단거리 염기서열 원시데이터를 확보
양귀비(Papaver rhoeas) 등 3종 조추출물의 기능성 탐색
영농 폐비닐 분해 활성 우수 미생물 또는 미생물군 선발
여성농업인센터 역할 재정립 및 운영 활성화 도출
소식재배 이앙기 식부장치 개량 및 보완
화상병 감염기간을 추정하고 유입경로 및 확산 경로를 분석
1세대 스마트팜 기술 적용 고온 극복형 온실 환경 모니터링 시스템 구축 시험 및 한국형 2세대 스마트 온실 플랫폼 적용

(출처 : 요약서 5p)

Abstract ▼

[제 1세부과제]
Purpose&Contents
The genome size and characteristics of Papaver rhoeas was determined as a preliminary analysis for genome project. Also, the chloroplaste genome was assembled and annotated.
Results
○ Two strains of P. rhoeas were selected as material.
○ Genomic raw reads we

[제 1세부과제]
Purpose&Contents
The genome size and characteristics of Papaver rhoeas was determined as a preliminary analysis for genome project. Also, the chloroplaste genome was assembled and annotated.
Results
○ Two strains of P. rhoeas were selected as material.
○ Genomic raw reads were sequenced using Illumina platform.
○ According to K-mer frequency analysis, the genome size was estimated as 2.47~2.48Gb and the heterozygosity level was as high as 1.54~1.79%.
○ The chloroplast genome was 152,947~152,948bp in length and contains 110 unique genes including 78 protein-coding genes, 4 rRNA genes, 28 tRNA genes.
○ The characterization of chloroplast genome and comparative analysis was presented in the KOGO conference and the obtained genomic raw reads were registered in the NABIC database.
Expected Contribution
○ The characteristics of P. rhoeas can be used to determine the strategy of genome project such as sequencing platform and analytic program.
○ The chloroplast genome sequence can serve as fundamental genomic resource for phylogenetic and evolutionary study of Papaver species and for developing molecular markers for species’ identification.
(출처 : Summary 8p)

[제 2세부과제]
Purpose&Contents
Activity assesment of medicinal material in Papaver rhoeas
Results
○ This study examined the antioxidant activities of 50% ethanol extract from Papaver L.(POL). The antioxidant activities of the POL extract was measured by evaluating the DPPH and the ABTS radical scavenging assay. The protective effects for H2O2-treated HepG2 cells were evaluated based on the cell viability, intracellular ROS scavenging activity. The DPPH and ABTS radical scavenging activities were dose-dependently increased by treatment of the POL extract. The POL extract increased the cell viability and intracellular ROS scavenging activity in HepG2 cells. Our results suggest that the POL has antioxidant and hepatoprotective effects against oxidative stress.
○ Papaver L.(POL) is an annual plant that is distributed in many parts of the world, especially the tropical and subtropical areas. POL has some pharmacological properties such as analgesic, antibacterial, ant-inflammatory and antioxidant activities. This study investigated the anticancer activities of 50% ethanol extract of POL (POLE) against HepG2 human hepatocellular carcinoma cells. The cytotoxicity of POLE was screened in HepG2 cells using water soluble tetrazolium salt (WST) and lactate dehydrogenase (LDH) analyses. Further the morphological changes in HepG2 cells after exposure to POLE was viewed under fluorescence and inverted light microscope. The apoptotic cell was measured by Hoechst staining. The caspase-3 and -9 activities were assessed by colorimetric assay. Overall analyses revealed that treatment with POLE for 48 hr can inhibit the viability of HepG2 cells. Incubation with POLE for 48 h significantly increased the total apoptotic cells compared to the control. Treatment with POLE for 48 h triggered both caspase-3 and -9 activities in a concentration-dependent pattern. Our results suggested that POLE offer potential against hepatocellular cancer through modulation caspase-dependent pathway.
Expected Contribution
○ Papaver rhoeas can be a potential candidate as medicinal materials
(출처 : Summary 26p)
[제 3세부과제]
Purpose&Contents
○ Preliminary test for the development of the disposal techniques of waste plastics using microorganisms
Results
○ Examination of experimental techniques for analysis of microbial degradative activity against plastics
○ Analysis of the degradative activities against plastics for reported microorganisms
○ Isolation of microbial strains or communities having degradative activities against agricultural waste plastics
Expected Contribution
○ Provision of microbial resources and research methods for he development of the disposal techniques of waste plastics using microorganisms
(출처 : Summary 55p)

[제 4세부과제]
Purpose & Contents
○ Reestablishing the role of Center for women farmers, deriving operation activation and developing manuals
- Analysis of status of Center for women farmers in nationwide and legal grounds
- Business diagnosis and evaluation of Center for women farmers in each city and province
- Analysis of Central Government consigned center operation cases
- Development of manual for Center for women farmers
Results
○ Reestablishing the role of Center for women farmers and deriving operation activation
- Comparison of operating guidelines and ordinances of Center for women farmers by province
- Gather opinions from staff to reestablish the role of Center for women farmers and revitalize operation: 4 times
- Analysis of operation cases of Central Government consigned centers of other Ministries and Departments performing similar projects: 7 cases
- Publish a manual of Center for women farmers: 1 manual
Expected Contribution
○ Establish legal and institutional basis for stable operation of Center for women farmers
○ Enhancement of rural life satisfaction of women farmers through improvement of operational level of Center for women farmers
(출처 : Summary 101p)

[제 5세부과제]
Purpose&Contents
○ Improvement of planting accuracy of rice transplanter for spacious planting
Results
○ Improvement of planting device of rice transplanter
- Improvement of trajectory of planting device
○ Performance test of improved rice transplanter
- Analysis of planting accuracy of improved rice transplanter
Expected Contribution
○ Improvement of planting accuracy of rice transplanter
- Plant missing rate : (before) 9.5% → (after) 1.3(37seedlings/3.3m²)
(출처 : Summary 143p)

[제 6세부과제]
Purpose & Contents
In-depth analysis was conducted through on-site survey of the diseased farms in order to investigate the causes of the domestic outbreak of fireblight in Korea, in 2019. In order to examine whether problems in the field can be applied to the development of control techniques, we investigated the causes of spreading diseases, the possibility of mediating insects, and the resistance of pesticide to bacteria. In addition, this study attempted to determine the domestic applicability of bacteriophages recently registered overseas.
Results
In order to analyze the causes of outbreak of fireblight in 2019, the duration of infection was estimated and the funnel and spread path were analyzed. The disease has already been reported in an orchard with an old infection, confirming that the symptom of disease was not well understood by farmers. The presence of the bacteria of fire blight in insects was not clear, but epidemiological investigations confirmed their probabilities.
The isolates of fireblight were selected and the pesticide resistance investigations revealed that resistance to current drugs did not occur. This year, the cause of outbreak of fireblight was not a pesticide, but was reported late because farmers did not know the symptoms in already infected orchards, and the high temperature after flowering. The bacteriophage commercially available was introduced to confirm the activity of phage against domestic strains and the treatment conditions were investigated.
Expected Contribution
Through this study, the characteristics of the fireblight injuries in Korea can be identified and applied to the fireblight management project, which will be implemented from 2020, and can be used to develop the control technology that can be utilized in the field. In addition, the pruning tool disinfectant can be selected and immersion conditions can be used to effectively disinfect the pruning tool in the field. The test of the effectiveness of commercially available bacteriophages will be an important reference in establishing environmental conditions for phage when performing related tasks beginning in 2020.
(출처 : Summary 160p)

[제 7세부과제]
Purpose&Contents
○ Design of the environment control system to control integrated in the practical cooling greenhouse
○ Application of the Korean smartfarm platform for the second stage
Results
○ Design of the environmental monitoring system in the practical cooling greenhouse using the first stage technologies in the smartfarm
- Integrated monitoring system for the environmental information collected inside & outside the greenhouse
○ Test for the remote control system applying the first stage technologies in the smartfarm
- Design of the remote control system for actuators and environment management in the greenhouse
○ Application of the Korean smartfarm platform for the second stage
- The data collection and user interface monitoring based on the cloud
Expected Contribution
○ Utilization of the platform research that connects integrated environment control system to cloud of the study on upgrading standardization to apply the smartfarm of the communication standardization in 2020
○ expected effects
- Preceding research for the high-height design in the greenhouse and spread of the temperature control function in the summer
- The standardization of the environment information collection, remote control, and cloud communication in the practical cooling greenhouse and the establishment of the infrastructure
(출처 : Summary 188p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 5
• 목차 ... 6
• 제 1 세부 꽃양귀비 유전체 특성검정 및 해독전략 수립 ... 7
• 국 문 요 약 문 ... 7
• Summary ... 8
• 제 1 장 연구 개발 과제의 개요 ... 9
• 제 2 장 연구 수행 내용 및 결과 ... 11
• 제 3 장 목표달성도 및 관련분야 기여도 ... 20
• 제 4 장 연구 결과의 활용 계획 ... 21
• 제 5 장 연구 개발 결과의 보안 등급 ... 21
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 21
• 제 7 장 연구개발과제의 대표적 연구실적 ... 21
• 제 8 장 기타사항 ... 21
• 제 9 장 참고문헌 ... 22
• 제 2 세부 꽃양귀비 기능성 탐색 및 항염·항산화 활성 평가 ... 25
• 국 문 요 약 문 ... 25
• Summary ... 26
• 제 1 장 연구개발 과제의 개요 ... 27
• 제 2 장 연구 수행 내용 및 결과 ... 28
• 제 3 장 목표달성도 및 관련분야 기여도 ... 52
• 제 4 장 연구 결과의 활용 계획 ... 52
• 제 5 장 연구 개발 결과의 보안 등급 ... 52
• 제 6 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 53
• 제 7 장 연구개발과제의 대표적 연구실적 ... 53
• 제 8 장 기타사항 ... 53
• 제 9 장 참고문헌 ... 53
• 제 3 세부 폐플라스틱 분해 기술 개발을 위한 미생물 탐색 및 분해력 검정 ... 55
• 국 문 요 약 문 ... 55
• Summary ... 55
• 제 1 장 연구 개발 과제의 개요 ... 56
• 제 2 장 연구 수행 내용 및 결과 ... 60
• 제 3 장 목표달성도 및 관련분야 기여도 ... 97
• 제 4 장 연구 결과의 활용 계획 ... 97
• 제 5 장 연구 개발 결과의 보안 등급 ... 98
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 98
• 제 7 장 연구개발과제의 대표적 연구실적 ... 99
• 제 8 장 기타사항 ... 99
• 제 9 장 참고문헌 ... 100
• 제 4 세부 여성농업인센터 역할 재정립 및 운영 매뉴얼 개발 연구 ... 101
• 국 문 요 약 문 ... 101
• Summary ... 101
• 제 1 장 연구 개발 과제의 개요 ... 102
• 제 2 장 연구 수행 내용 및 결과 ... 106
• 제 3 장 목표달성도 및 관련분야 기여도 ... 140
• 제 4 장 연구 결과의 활용 계획 ... 141
• 제 5 장 연구 개발 결과의 보안 등급 ... 141
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 141
• 제 7 장 연구개발과제의 대표적 연구실적 ... 141
• 제 8 장 기타사항 ... 141
• 제 9 장 참고문헌 ... 142
• 제 5 세부 벼 밀묘 소식재배 이앙기 식부부 개선 연구 ... 143
• 국 문 요 약 문 ... 143
• Summary ... 143
• 제 1 장 연구 개발 과제의 개요 ... 144
• 제 2 장 연구 수행 내용 및 결과 ... 145
• 제 3 장 목표달성도 및 관련분야 기여도 ... 156
• 제 4 장 연구 결과의 활용 계획 ... 157
• 제 5 장 연구 개발 결과의 보안 등급 ... 157
• 제 6 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 157
• 제 7 장 연구개발과제의 대표적 연구실적 ... 157
• 제 8 장 기타사항 ... 157
• 제 9 장 참고문헌 ... 158
• 제 6 세부 2019년 화상병 발생 과원의 발생 요인 및 해외 방제약제 적용성 검토 연구 ... 159
• 국 문 요 약 문 ... 159
• Summary ... 160
• 제 1 장 연구 개발 과제의 개요 ... 161
• 제 2 장 연구 수행 내용 및 결과 ... 162
• 제 3 장 목표달성도 및 관련분야 기여도 ... 185
• 제 4 장 연구 결과의 활용 계획 ... 185
• 제 5 장 연구 개발 결과의 보안 등급 ... 185
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 186
• 제 7 장 연구개발과제의 대표적 연구실적 ... 186
• 제 8 장 기타사항 ... 186
• 제 9 장 참고문헌 ... 186
• 제 7 세부 고온 극복형 온실 대상 1세대 스마트팜 기술 적용 및 클라우드 기반 데이터 수집 플랫폼 구축 연구 ... 187
• 국 문 요 약 문 ... 187
• Summary ... 188
• 제 1 장 연구 개발 과제의 개요 ... 189
• 제 2 장 연구 수행 내용 및 결과 ... 190
• 제 3 장 목표달성도 및 관련분야 기여도 ... 203
• 제 4 장 연구 결과의 활용 계획 ... 204
• 제 5 장 연구 개발 결과의 보안 등급 ... 204
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 204
• 제 7 장 연구개발과제의 대표적 연구실적 ... 205
• 제 8 장 기타사항 ... 205
• 제 9 장 참고문헌 ... 205
• 끝페이지 ... 206