Abstract ▼

□ Purpose&Contents
▪ Development of curcumin-containing microsphere aggregated cell cluster for treatment of diabetes
- Preparation and characterization of curcumin-microsphere
- Optimization of making cell cluster
- Preparation of curcumin-containing microsphere aggregated cell cluster<

□ Purpose&Contents
▪ Development of curcumin-containing microsphere aggregated cell cluster for treatment of diabetes
- Preparation and characterization of curcumin-microsphere
- Optimization of making cell cluster
- Preparation of curcumin-containing microsphere aggregated cell cluster
- Analysis of anti-apoptosis effect of curcumin-containing microsphere aggregated cell cluster
- Evaluation of viability and functionality of curcumin-containing microsphere aggregated cell cluster
- Application of microsphere aggregated cell cluster making system to stem cell and hepatocytes

□ Results
▪ Self-assembled heterospheroids of curcumin-loaded PLGA microspheres and pancreatic islets were formed by using hanging drop method.
▪ Fluorescence and confocal microscopy revealed a uniform distribution of microspheres and islet single cells.
▪ The method of forming heterospheroids did not affect insulin secretion in basal and stimulated glucose conditions.
▪ The long-term release of curcumin in the islet microenvironment effectively inhibited islet apoptosis as indicated by decreased red fluorescence in the heterospheroids.
▪ In vivo transplantation and imaging of the heterospheroids into the kidney subcapsule of mice confirmed the localization of islets and microspheresafter the transplantation.
▪ Quercetin microsphere effectively have improved the survivla time of transplanted spheroid in diabetic mouse model.
▪ The technology of making heterospheroids with drug carriers and cells is applicable to other cell therapies such as hepatocyte and stem cell.

□ Expected Contribution
▪ Locally co-delivery of drug and cell which help to enhance delivered cell viability
▪ Apply to other kinds of cell (stem cell and hepatocytes) delivery for the treatment of disease model
▪ Co-delivery Imaging agent with cell cluster for theragnosis
▪ Development of new methodology for effective delivery of bio-medicine to target site

(source : SUMMARY 13p)

목차 Contents

• 표지 ... 1
• 보고서요약 ... 2
• 제 출 문 ... 10
• 보고서 요약서 ... 11
• 국문 요약문 ... 12
• SUMMARY ... 13
• 목차 ... 14
• 1. 연구개발과제의 개요 ... 15
• 1-1. 연구개발 목적 ... 15
• 1-2. 연구개발의 필요성 ... 15
• 1-3. 연구개발 범위 ... 17
• 2. 국내외 기술개발 현황 ... 21
• 3. 연구수행 내용 및 결과 ... 22
• 4. 목표달성도 및 관련분야 기여도 ... 34
• 4-1. 목표달성도 ... 34
• 4-2. 관련분야 기여도 ... 35
• 5. 연구결과의 활용계획 ... 36
• 4-1. 연구개발 성과의 활용 방안 ... 36
• 4-2. 기대 효과 및 파급효과 ... 36
• 6. 연구과정에서 수집한 해외과학기술정보 ... 37
• 7. 연구개발성과의 보안등급 ... 37
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 37
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 37
• 10. 연구개발과제의 대표적 연구실적 ... 38
• 11. 기타사항 ... 38
• 12. 참고문헌 ... 38
• 끝페이지 ... 39