[논문]Antioxidant Activity-of Curcuma Longa L., Novel Foodstuff

Choi, Hae-Yeon

Antioxidant Activity-of Curcuma Longa L., Novel Foodstuff 원문보기

Molecular & cellular toxicology, v.5 no.3, 2009년, pp.237 - 242

Choi, Hae-Yeon (Department of Food and Nutrition, Sookmyung Women's University)

Abstract ▼ AI-Helper

The potential antioxidant activities of different fractions from methanolic extract of Curcuma longa L. were assayed in vitro. All of the fractions exception of n-hexane and $H_2O$ showed a strong antioxidant activity, especially the ethylacetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}=9.86\;{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed concentration dependency, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in this studies. Additionally, when the total phenolic contents of fractions were measured, the EtOAc fraction contained the highest level. Meanwhile, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic contents, suggesting that fractions obtained from the methanolic extract of Curcuma longa L. have wide potential for use as sources of antioxidant material.

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제안 방법

In this study, antioxidative capacities, total phenolic contents, and their correlations of the various fractions from methanolic extract of Curcuma longa L. were examined with useful methods.
have been associated to the antioxidant activity1618. So, this study examined the antioxidative activity of various fractions from methanolic extract of Curcuma longa L. against DPPH, ABTS, and FRAP and to estimate total phenolic contents. Additionally, the correlation between the total phenolic contents in the tested fractions and their antioxidant activities was investigated.

대상 데이터

The Curcuma longa L. was obtained from local medicine stores in Seoul, Korea. The dried powder (500 g) was extracted with solvent of MeOH for 3 hr (1 L x 3).

데이터처리

Duncan's new multiple range test was used to determine significant differences. Correlations amon_용data obtained were calculated using Pearson's correlation coefficient (R).
Statistical significance was evaluated by one-way analysis (ANOVA) of variance using SAS version 9.1. Duncan's new multiple range test was used to determine significant differences.

이론/모형

The FRAP assay was performed according to the modified Benzie and Strain method31. The stock solutions included 300 mM acetate buffer (3.
The phenolic compounds were determined by the Folin-Ciocalteu method, which was adapted from Swain and Hillis32. The 150 _μ1.

성능/효과

It reported that these assays require relatively standard eqipmant and yield the fast and most reproducible results22. From the results, it was demonstrated that the EtOAc fraction from methanolic extract of Curcuma longa L. possessed an excellent antioxidant activity based on the DPPH, ABTS, and FRAP assay.
Phenolic compounds having one or more aromatic rings bearing one or more hydroxyl grops can potentially quench free radicals by forming resonance-stabilized phenoxyl radicals and therefore have redox properties23, 24. In our results, strong positive correlations were found between DPPH, ABTS, and FRAP assays and total phenolic contents. These results indicated a relationship between phenolic compound concentration in various fractions from methanolic extract of Curcuma longa L.
28 _μg/mL, respectively. In this study, trolox and vitamin C were measured as the positive controls, showing the IC₅₀ values of 17.19 and 2.44 |ig/mL, respectively.
001). Results obtained from DPPH, ABTS, and FRAP assays were also correlated to the total phenolic compound concentration (7?=0.979, R= 0.914, and R=0.960, P<0.001).
assays. Significant correlations were found between the various methods used to determine the antioxidant potential, especially between ABTS and FRAP assays (R=0.984, P< 0.001), DPPH and ABTS assays 0.954,0.001), and DPPH and FRAP assays (7?=0.969, 0.001). Results obtained from DPPH, ABTS, and FRAP assays were also correlated to the total phenolic compound concentration (7?=0.

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