Primary antibodies to two ubiquitous components of basement membrane, against to type Ⅳ collagen and laminin, were applied to formalin-fixed and paraffin -embeded tissue sections of a variety of tumorous conditions, benign tumor, dysplastic lesions, intraepithelial neoplasms and invasive carcinomas ...
Primary antibodies to two ubiquitous components of basement membrane, against to type Ⅳ collagen and laminin, were applied to formalin-fixed and paraffin -embeded tissue sections of a variety of tumorous conditions, benign tumor, dysplastic lesions, intraepithelial neoplasms and invasive carcinomas to determine the status of basement membrane alteration by immunohistochemical and ultrastructural examinations. Included lesions of this study were breast (fibrocystic disease, fibroadenoma, intraductal carcinoma, infiltrating ductal carcinoma), colon(adenoma, adenocarcinoma, well to poorly differentiated), stomach(adencoarcinoma, well to poorly differentiated), cervix(dysplasia, mcroinvasive and invasive squamous cell carcinoma) and metastatic adenocarcinoma in lymph nodes (stomach and colon). By immunoperoxidase techniques, the lesions of tumorous conditions, benign tumors and dysplasias showed intact basement membranes with linear staining pattern for the type Ⅳ collagen and laminin The maprity of the invasive carcinomas, in contrast, lacked immunoreactivity for the basement membrane components. In case of in situ carcinoma with microinvasion, there was thinning, fragmentation and distruption of the basement membrane in the foci of microinvasion but not elsewhere. The ultrastructural findings examined showed similar patterns of immunohistochemical results. There was no differences of immunostaining properties of the basement membrane between well and poorly differentiated neoplasm. Also, there was no immunohistochemical differences between primary and metastatic adenocarcinoma.Utilizing antibodies against type Ⅳ collagen and laminin aids in both understanding the invasiveness and the recognition of its presence in various tumor tissue sections.
Primary antibodies to two ubiquitous components of basement membrane, against to type Ⅳ collagen and laminin, were applied to formalin-fixed and paraffin -embeded tissue sections of a variety of tumorous conditions, benign tumor, dysplastic lesions, intraepithelial neoplasms and invasive carcinomas to determine the status of basement membrane alteration by immunohistochemical and ultrastructural examinations. Included lesions of this study were breast (fibrocystic disease, fibroadenoma, intraductal carcinoma, infiltrating ductal carcinoma), colon(adenoma, adenocarcinoma, well to poorly differentiated), stomach(adencoarcinoma, well to poorly differentiated), cervix(dysplasia, mcroinvasive and invasive squamous cell carcinoma) and metastatic adenocarcinoma in lymph nodes (stomach and colon). By immunoperoxidase techniques, the lesions of tumorous conditions, benign tumors and dysplasias showed intact basement membranes with linear staining pattern for the type Ⅳ collagen and laminin The maprity of the invasive carcinomas, in contrast, lacked immunoreactivity for the basement membrane components. In case of in situ carcinoma with microinvasion, there was thinning, fragmentation and distruption of the basement membrane in the foci of microinvasion but not elsewhere. The ultrastructural findings examined showed similar patterns of immunohistochemical results. There was no differences of immunostaining properties of the basement membrane between well and poorly differentiated neoplasm. Also, there was no immunohistochemical differences between primary and metastatic adenocarcinoma.Utilizing antibodies against type Ⅳ collagen and laminin aids in both understanding the invasiveness and the recognition of its presence in various tumor tissue sections.
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