Abstract ▼ AI-Helper

These experiments were designed to investigate the molecular cloning of renin cDNA synthesized from renin mRNA of mouse submaxillary gland, and thus to characterize the renin protein expressed by the renin cDNA. The results obtained were as follows: 1. The total RNA was isolated from the mouse subma...

These experiments were designed to investigate the molecular cloning of renin cDNA synthesized from renin mRNA of mouse submaxillary gland, and thus to characterize the renin protein expressed by the renin cDNA. The results obtained were as follows: 1. The total RNA was isolated from the mouse submaxillary gland by the method of GIT/CsCl, and the mRNA was separated by using oligo(dT) cellulose and poly(U) sepharose 4B matrix. 2. primer Ⅰ and primer Ⅱ was synthesized from renin cDNA, and cDNA was transcribed reversely and amplified by the method of polymerase chain reaction(PCR) 3. The recombinant plasmid(pUCR1) was constructed by ligating the cDNA into the pUC19 plasmid of E. coli vector. 4. pUCR1 was transformed into E. coli(JM103) and the recombinant plasmid was identified with Southern blot analysis. 5. The renin gene expression was identified with polyacrylamide gel electrophoresis and the molecular weight of renin was 45,000.

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