This study was carried out to purify and analyze components of fucoidan in polysaccharides from two brown seaweed species(Laminaria japonica Ⅰ·Ⅱand Hizikia fusiformis Ⅰ·Ⅱ). For crude fucoidan preparation, Laminaria Japonica(Ⅰ·Ⅱ), Hizikia fusiformis(Ⅰ·Ⅱ) were treated preliminarily with 85% EtOH to re...
This study was carried out to purify and analyze components of fucoidan in polysaccharides from two brown seaweed species(Laminaria japonica Ⅰ·Ⅱand Hizikia fusiformis Ⅰ·Ⅱ). For crude fucoidan preparation, Laminaria Japonica(Ⅰ·Ⅱ), Hizikia fusiformis(Ⅰ·Ⅱ) were treated preliminarily with 85% EtOH to remove soluble monosaccharides and pigments. After extracted fucoidan with dilute hydrochloric acid, Crude fucoidan Laminaria japonica Ⅰ(ⅠCFL), crude fucoidan taminaria Japonica Ⅱ(ⅡCFL), crude fucoidan Hizikia fusiformis Ⅰ(ⅠCFH) and crude fucoidan Hizikia fusiformis Ⅱ(ⅡCFH) were obtained. The yields of ⅠCFL, ⅡCFL, ⅠCFH and ⅡCFH were 1.08%, 1.9%, 1.31% and 1.4 % respectively. The yields of crude fucoidans from Laminaria japonica was higher than that from Hizikia fusiformis. ⅠCFL was fucose, xylose, mannose, glucose, galactose, manuronic acid and guluronic acid and ⅡCFL was fucose, xylose, mannose, galactose, manuronic acid and guluronic acid and ⅠCFH and ⅡCFH were fucose, xylose, mannose, glucose, galactose, manuronic acid guluronic acid and galacturonic acid. ⅠCFL, ⅡCFL, ⅠCFH, ⅡCFH were fractionated by DEAE-Sephadex A-25 column ion-exchange chromatography into four aliquots ; Laminaria japonica Ⅰfraction 1(ⅠLFr-1), Laminaria japonica Ⅰfraction 2(ⅠLFr-2), Laminaria japonica Ⅰfraction 3(ⅠLFr-3), Laminaria Japonica Ⅰfraction 4(ⅠLFr-4), four aliquots ; Laminaria Japonica Ⅱfraction 1(ⅡLFr-1), Laminaria japonica Ⅱfraction 2(ⅡLFr-2), Laminaria Japonica Ⅱfraction 3(ⅡLFr-3), Laminaria Japonica Ⅱfraction 4(ⅡLFr-4), three aliquots ; Hizikia fusiformis Ⅰfraction 1(ⅠHFr-1), Hizikia fusiformis Ⅰfraction 2(ⅠHFr-2), Hizikia fusiformis Ⅰfraction 3(ⅠHFr-3) and four aliquots ; Hizikia fusiformis Ⅱfraction 1(ⅡHFr-1), Hizikia fusiformis Ⅱfraction 2(ⅡHFr-2), Hizikia fusiformis Ⅱfraction 3(ⅡHFr-3) and Hizikia fusiformis Ⅱfraction 4(ⅡHFr-4). Electrophoresis experiments were that ⅠLFr-1, ⅡLFr-1, ⅠHFr-1 and ⅡHFr-1 were single alginic acid component. ⅠLFr-2, ⅡLFr-2, ⅠHFr-3, ⅡHFr-3 and ⅡHFr-4 were single component. ⅠLFr-3, ⅠLFr-4, ⅡLFr-3 and ⅡLFr-4 were two components. The major components in ⅠLFr-1 were manuronic acid and guluronic acid. Major components of ⅠL Fr-2, ⅠLFr-3, ⅠLFr-4 were fucose, mannose, galactose, manuronic acid and guluronic acid in addition to glucose and xylose. The major components in ⅡILFr-1 were manuronic acid and guluronic acid. Major components of ⅡLFr-2, ⅡLFr-3, ⅡLFr-4 were fucose, galactose, manuronic acid and guluronic acid in addition to mannose. ⅠHFr-1 was manuronic acid and guluronic acid. The major components in ⅠHFr-2 were glucose, manuronic acid and guluronic acid, The major components in ⅠHFr-3 contained fucose, xylose, manose, galactose, manuronic acid, guluronic acid and galacturonic acid. The major components in ⅡHFr-1 were manuronic acid and guluronic acid. ⅡHFr-2 consisted of manuronic acid, guluronic acid and glucose, The major components in ⅡHFr-3 and ⅡHFr-4 were fucose, xylose, galactose, manuronic acid, guluronic acid and galacturonic acid. The percentage of the protein, uronic acid and sol fates of crude fucoidans were that ⅠCFL was 4.78, 6.82 and 50.8, and ⅠCPH was 3.70, 7.94 and 28.7, and ⅡCFL was 4.78, 6.61 and 43.7, and ⅡCFH was 9.80, 6.19 and 22.9 respectively. The percent contents of the fucose, xylose, mannose, galactose, glucose of crude fucoidans were that I CFL was 85.8, 1.5, 1.8, 9.1 and 1.7, and ⅠCFH was 62,2, 7.3, 3.3, 24.5 and 2.8, and ⅡCFL was 87.3, 0.4, 2.4, 9.2 and trace, and ⅡCFH was 35.4, 4.9, 1.8, 22.3 and 35.7 respectively. IR spectrum was suggested that S=0 stretching vibration at 1122-1259cm^(-1) is from ester sulfate and C-0-S stretching vibration at 860cm^(-1) and carbonyl stretching vibration at 1625cm-1 are indicated the presence of uronic acid.
This study was carried out to purify and analyze components of fucoidan in polysaccharides from two brown seaweed species(Laminaria japonica Ⅰ·Ⅱand Hizikia fusiformis Ⅰ·Ⅱ). For crude fucoidan preparation, Laminaria Japonica(Ⅰ·Ⅱ), Hizikia fusiformis(Ⅰ·Ⅱ) were treated preliminarily with 85% EtOH to remove soluble monosaccharides and pigments. After extracted fucoidan with dilute hydrochloric acid, Crude fucoidan Laminaria japonica Ⅰ(ⅠCFL), crude fucoidan taminaria Japonica Ⅱ(ⅡCFL), crude fucoidan Hizikia fusiformis Ⅰ(ⅠCFH) and crude fucoidan Hizikia fusiformis Ⅱ(ⅡCFH) were obtained. The yields of ⅠCFL, ⅡCFL, ⅠCFH and ⅡCFH were 1.08%, 1.9%, 1.31% and 1.4 % respectively. The yields of crude fucoidans from Laminaria japonica was higher than that from Hizikia fusiformis. ⅠCFL was fucose, xylose, mannose, glucose, galactose, manuronic acid and guluronic acid and ⅡCFL was fucose, xylose, mannose, galactose, manuronic acid and guluronic acid and ⅠCFH and ⅡCFH were fucose, xylose, mannose, glucose, galactose, manuronic acid guluronic acid and galacturonic acid. ⅠCFL, ⅡCFL, ⅠCFH, ⅡCFH were fractionated by DEAE-Sephadex A-25 column ion-exchange chromatography into four aliquots ; Laminaria japonica Ⅰfraction 1(ⅠLFr-1), Laminaria japonica Ⅰfraction 2(ⅠLFr-2), Laminaria japonica Ⅰfraction 3(ⅠLFr-3), Laminaria Japonica Ⅰfraction 4(ⅠLFr-4), four aliquots ; Laminaria Japonica Ⅱfraction 1(ⅡLFr-1), Laminaria japonica Ⅱfraction 2(ⅡLFr-2), Laminaria Japonica Ⅱfraction 3(ⅡLFr-3), Laminaria Japonica Ⅱfraction 4(ⅡLFr-4), three aliquots ; Hizikia fusiformis Ⅰfraction 1(ⅠHFr-1), Hizikia fusiformis Ⅰfraction 2(ⅠHFr-2), Hizikia fusiformis Ⅰfraction 3(ⅠHFr-3) and four aliquots ; Hizikia fusiformis Ⅱfraction 1(ⅡHFr-1), Hizikia fusiformis Ⅱfraction 2(ⅡHFr-2), Hizikia fusiformis Ⅱfraction 3(ⅡHFr-3) and Hizikia fusiformis Ⅱfraction 4(ⅡHFr-4). Electrophoresis experiments were that ⅠLFr-1, ⅡLFr-1, ⅠHFr-1 and ⅡHFr-1 were single alginic acid component. ⅠLFr-2, ⅡLFr-2, ⅠHFr-3, ⅡHFr-3 and ⅡHFr-4 were single component. ⅠLFr-3, ⅠLFr-4, ⅡLFr-3 and ⅡLFr-4 were two components. The major components in ⅠLFr-1 were manuronic acid and guluronic acid. Major components of ⅠL Fr-2, ⅠLFr-3, ⅠLFr-4 were fucose, mannose, galactose, manuronic acid and guluronic acid in addition to glucose and xylose. The major components in ⅡILFr-1 were manuronic acid and guluronic acid. Major components of ⅡLFr-2, ⅡLFr-3, ⅡLFr-4 were fucose, galactose, manuronic acid and guluronic acid in addition to mannose. ⅠHFr-1 was manuronic acid and guluronic acid. The major components in ⅠHFr-2 were glucose, manuronic acid and guluronic acid, The major components in ⅠHFr-3 contained fucose, xylose, manose, galactose, manuronic acid, guluronic acid and galacturonic acid. The major components in ⅡHFr-1 were manuronic acid and guluronic acid. ⅡHFr-2 consisted of manuronic acid, guluronic acid and glucose, The major components in ⅡHFr-3 and ⅡHFr-4 were fucose, xylose, galactose, manuronic acid, guluronic acid and galacturonic acid. The percentage of the protein, uronic acid and sol fates of crude fucoidans were that ⅠCFL was 4.78, 6.82 and 50.8, and ⅠCPH was 3.70, 7.94 and 28.7, and ⅡCFL was 4.78, 6.61 and 43.7, and ⅡCFH was 9.80, 6.19 and 22.9 respectively. The percent contents of the fucose, xylose, mannose, galactose, glucose of crude fucoidans were that I CFL was 85.8, 1.5, 1.8, 9.1 and 1.7, and ⅠCFH was 62,2, 7.3, 3.3, 24.5 and 2.8, and ⅡCFL was 87.3, 0.4, 2.4, 9.2 and trace, and ⅡCFH was 35.4, 4.9, 1.8, 22.3 and 35.7 respectively. IR spectrum was suggested that S=0 stretching vibration at 1122-1259cm^(-1) is from ester sulfate and C-0-S stretching vibration at 860cm^(-1) and carbonyl stretching vibration at 1625cm-1 are indicated the presence of uronic acid.
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