계면활성제는 이온성에 따라 양이온, 음이온, 양쪽성, 비이온으로 구분되며 산업, 세제 화장품, 의약품 등의 가정용 화학물질들에 널리 사용하고 있다. 본 실험에서는 4 군중 6종류의 계면활성제[Cetyltrimetyl ammonium chloride(CTAC), Dialkyl diester hydroxyethyl methyl ammonium methosulfate(TE90): Cationic, Linear alkybenzene sulfonate(...
계면활성제는 이온성에 따라 양이온, 음이온, 양쪽성, 비이온으로 구분되며 산업, 세제 화장품, 의약품 등의 가정용 화학물질들에 널리 사용하고 있다. 본 실험에서는 4 군중 6종류의 계면활성제[Cetyltrimetyl ammonium chloride(CTAC), Dialkyl diester hydroxyethyl methyl ammonium methosulfate(TE90): Cationic, Linear alkybenzene sulfonate(LAS), Sodium lauryl sulfate(SLS): Anionic, Cocoamphocarboxy glycinate (HiaC2M): Amphoteric, TritonX 100: Nonionic]의 세포독성을 L929, mouse fibroblast cell line과 SV80, human fibroblast cell line을 이용하여 3-(4,5-dinethythiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromie(MTT)법, neutral red(NR)법, sulforhodamine B(SRB), Lactate dehydrogenaseleakage, 형태적 관찰 등을 통해 비교 조사하였다. 그 결과 SV80, L929 두 세포 모두에서 CTAC(+)> TE(+)> LAS(-)> SLS(-)> TritonX100(non)> HiaC2M(±)순으로 in vivo Draize test와 다른 세포들을 이용한 결과들과 유사한 결과를 나타냈다. 사용된 4가지 독성 검정 방법은 모두 처리농도에 의존적인 유사성 독성도를 나타냈고, 세포소기관을 중심으로 평가된 MTT, NR 방법은 LDH leakage와 SRB 방법보다 민감하고 MTT_(50), NR_(50)을 통해 정량적인 결과를 얻을 수 있었다. 이러한 결과들을 통해 SV80, L929세포를 배양하여 MTT, NR 방법으로 독성을 평가하는 방법은 계속해서 새롭게 개발되고 있는 계면활성제들의 독성을 신속하게 평가하는데 유용하게 사용될 수 있는 방법이라고 생각된다.
계면활성제는 이온성에 따라 양이온, 음이온, 양쪽성, 비이온으로 구분되며 산업, 세제 화장품, 의약품 등의 가정용 화학물질들에 널리 사용하고 있다. 본 실험에서는 4 군중 6종류의 계면활성제[Cetyltrimetyl ammonium chloride(CTAC), Dialkyl diester hydroxyethyl methyl ammonium methosulfate(TE90): Cationic, Linear alkybenzene sulfonate(LAS), Sodium lauryl sulfate(SLS): Anionic, Cocoamphocarboxy glycinate (HiaC2M): Amphoteric, TritonX 100: Nonionic]의 세포독성을 L929, mouse fibroblast cell line과 SV80, human fibroblast cell line을 이용하여 3-(4,5-dinethythiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromie(MTT)법, neutral red(NR)법, sulforhodamine B(SRB), Lactate dehydrogenase leakage, 형태적 관찰 등을 통해 비교 조사하였다. 그 결과 SV80, L929 두 세포 모두에서 CTAC(+)> TE(+)> LAS(-)> SLS(-)> TritonX100(non)> HiaC2M(±)순으로 in vivo Draize test와 다른 세포들을 이용한 결과들과 유사한 결과를 나타냈다. 사용된 4가지 독성 검정 방법은 모두 처리농도에 의존적인 유사성 독성도를 나타냈고, 세포소기관을 중심으로 평가된 MTT, NR 방법은 LDH leakage와 SRB 방법보다 민감하고 MTT_(50), NR_(50)을 통해 정량적인 결과를 얻을 수 있었다. 이러한 결과들을 통해 SV80, L929세포를 배양하여 MTT, NR 방법으로 독성을 평가하는 방법은 계속해서 새롭게 개발되고 있는 계면활성제들의 독성을 신속하게 평가하는데 유용하게 사용될 수 있는 방법이라고 생각된다.
Surfactants are the major components of cleansing products, cosmectics and medical materials. It is well known that many surfactants cause irritation. The Draize test has been widely used to predict chemical irritation. However, this test has been criticized for lack of reproducibility, the subjecti...
Surfactants are the major components of cleansing products, cosmectics and medical materials. It is well known that many surfactants cause irritation. The Draize test has been widely used to predict chemical irritation. However, this test has been criticized for lack of reproducibility, the subjective nature of scoring and evaluating the response, long assay duration, relatively high cost and particularly the use of animals. So, a large number of in vitro systems have been investigated as possible alternatives for the Draize test. This study was carried out to investigate the cytotoxicity of surfactants using two cell lines, human fibroblast cells SV80 and mouse fibroblast cells L929. The cytotoxicity of 6 surfactants - Cetyltrimethyl ammonium chroride(CTAC), Dialkyl diester hydroxyethyl methylammonium methosulfate (TE90): Cationic, Linear alkylbenzene sulfonate(LAS), Sodium lauryl sulfate(SLS): Anionic, Cocoamphocarboxy glycinate (IIiaC2M): Amphoteric, TritonX100: Nonionic- was evaluated by Neutral Red, 3-(4,5-dimethythiazol-2-yl) -2,5-diphenyl-2H- tetrazolium bromide (MTT), sulforhodamine B(SRB) assay, LDH leakage, and cell morphology. Four endpoints used to assess toxicity gave sililar dose/response curve. However, the MTT and NR assay were more sensitive than LDH leakage and SRB assay in general. As the result, comparative surfactant cytotoxicity was CTAC(+)> TE(+)> LAS(-)> SLS(-)> TritonX100(non)> HiaC2M(±). The results were similar to those of some cytotoxicity experiments performed on differents cell types and in vivo Draize test. Therefore, in vitro cytotoxicity assay using L929 cells and SV80 cells with MTT and NR assay may be used to rank the cytotoxicity potential of surfactants.
Surfactants are the major components of cleansing products, cosmectics and medical materials. It is well known that many surfactants cause irritation. The Draize test has been widely used to predict chemical irritation. However, this test has been criticized for lack of reproducibility, the subjective nature of scoring and evaluating the response, long assay duration, relatively high cost and particularly the use of animals. So, a large number of in vitro systems have been investigated as possible alternatives for the Draize test. This study was carried out to investigate the cytotoxicity of surfactants using two cell lines, human fibroblast cells SV80 and mouse fibroblast cells L929. The cytotoxicity of 6 surfactants - Cetyltrimethyl ammonium chroride(CTAC), Dialkyl diester hydroxyethyl methylammonium methosulfate (TE90): Cationic, Linear alkylbenzene sulfonate(LAS), Sodium lauryl sulfate(SLS): Anionic, Cocoamphocarboxy glycinate (IIiaC2M): Amphoteric, TritonX100: Nonionic- was evaluated by Neutral Red, 3-(4,5-dimethythiazol-2-yl) -2,5-diphenyl-2H- tetrazolium bromide (MTT), sulforhodamine B(SRB) assay, LDH leakage, and cell morphology. Four endpoints used to assess toxicity gave sililar dose/response curve. However, the MTT and NR assay were more sensitive than LDH leakage and SRB assay in general. As the result, comparative surfactant cytotoxicity was CTAC(+)> TE(+)> LAS(-)> SLS(-)> TritonX100(non)> HiaC2M(±). The results were similar to those of some cytotoxicity experiments performed on differents cell types and in vivo Draize test. Therefore, in vitro cytotoxicity assay using L929 cells and SV80 cells with MTT and NR assay may be used to rank the cytotoxicity potential of surfactants.
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