Tetrachloroethylene(PCE) is one of the most serious soil and groundwater pollutants due to widespread use in dry cleaning of textiles and solvent of chemical industry. PCE is recalcitrant under aerobic condition but can be dechlorinated to lower-chlorinated compounds, like trichloroethylene(TCE), di...
Tetrachloroethylene(PCE) is one of the most serious soil and groundwater pollutants due to widespread use in dry cleaning of textiles and solvent of chemical industry. PCE is recalcitrant under aerobic condition but can be dechlorinated to lower-chlorinated compounds, like trichloroethylene(TCE), dichloroethylene(DCE), vinyl chloride(VC) and ethylene, by a few microbes under anaerobic condition. The purpose of this study was to establish enrichment culture from various microcosms that are able to dechlorinate PCE under anaerobic condition and to investigate the microbial community connected with PCE dechlorination in enrichment culture by using molecular biological analysis, Polymerase chain reaction-Denaturing gradient gel electrophoresis(PCR-DGGE) method. Microbial community associate with anaerobic PCE dechlorination was obtained from various soil, anaerobic digestion sludge and leachate. Enrichment culture possessed PCE dechlorination activity was performed continual subculture in batch reactor using 50-mL serum bottles. The results of this study were as follows; PCE dechlorination activity of enrichment culture derived from various microcosms was investigated. 60 μM of PCE could be dechlorinate in culture from leachate, especially from landfill soil. As sequential subculture from the cultures was performed, enrichment culture could dechlorinate 60 μM of PCE via TCE to cis-DCE within 3 days was obtained. The effects of environmental condition such as temperature(20~45℃) and pH(4~10) on PCE dechlorination activity were investigated. These results suggested that the highest PCE dechlorination rate was observed at the temperature of 30 to 35℃ and pH 7. The enrichment culture could dechlorinate TCE but did not degrade other chlorinated aliphatic compounds, such as cis-DCE, trans-DCE, 1,1-dichloroethylene, 1,1-dichloroethane, 1,2-dichloroethane and 1,1,1- trichloroethane for a month. Microbial analysis of the PCE dechlorinating enrichment culture by using PCR-DGGE method showed that at least five microorganisms were related to the anaerobic PCE dechlorination. Bacterial DGGE band profiles of enrichment culture possessed PCE dechlorination activity(PCE+) compared with no possessed(PCE-,A, B, C and D) were distinguished by one band. It is assumed that strains of differential one band were related to PCE dechlorination directly. In conclusion, results from this study indicate that the anaerobic mixed culture was able to dechlorinate PCE to cis-DCE on bioremediation of soil and groundwater contaminated with chlorinated ethylene compounds, through bioaugmentation with microorganisms -obtaining enrichment culture.
Tetrachloroethylene(PCE) is one of the most serious soil and groundwater pollutants due to widespread use in dry cleaning of textiles and solvent of chemical industry. PCE is recalcitrant under aerobic condition but can be dechlorinated to lower-chlorinated compounds, like trichloroethylene(TCE), dichloroethylene(DCE), vinyl chloride(VC) and ethylene, by a few microbes under anaerobic condition. The purpose of this study was to establish enrichment culture from various microcosms that are able to dechlorinate PCE under anaerobic condition and to investigate the microbial community connected with PCE dechlorination in enrichment culture by using molecular biological analysis, Polymerase chain reaction-Denaturing gradient gel electrophoresis(PCR-DGGE) method. Microbial community associate with anaerobic PCE dechlorination was obtained from various soil, anaerobic digestion sludge and leachate. Enrichment culture possessed PCE dechlorination activity was performed continual subculture in batch reactor using 50-mL serum bottles. The results of this study were as follows; PCE dechlorination activity of enrichment culture derived from various microcosms was investigated. 60 μM of PCE could be dechlorinate in culture from leachate, especially from landfill soil. As sequential subculture from the cultures was performed, enrichment culture could dechlorinate 60 μM of PCE via TCE to cis-DCE within 3 days was obtained. The effects of environmental condition such as temperature(20~45℃) and pH(4~10) on PCE dechlorination activity were investigated. These results suggested that the highest PCE dechlorination rate was observed at the temperature of 30 to 35℃ and pH 7. The enrichment culture could dechlorinate TCE but did not degrade other chlorinated aliphatic compounds, such as cis-DCE, trans-DCE, 1,1-dichloroethylene, 1,1-dichloroethane, 1,2-dichloroethane and 1,1,1- trichloroethane for a month. Microbial analysis of the PCE dechlorinating enrichment culture by using PCR-DGGE method showed that at least five microorganisms were related to the anaerobic PCE dechlorination. Bacterial DGGE band profiles of enrichment culture possessed PCE dechlorination activity(PCE+) compared with no possessed(PCE-,A, B, C and D) were distinguished by one band. It is assumed that strains of differential one band were related to PCE dechlorination directly. In conclusion, results from this study indicate that the anaerobic mixed culture was able to dechlorinate PCE to cis-DCE on bioremediation of soil and groundwater contaminated with chlorinated ethylene compounds, through bioaugmentation with microorganisms -obtaining enrichment culture.
주제어
#혐기성 PCE 탈염소화 미생물 농화 배양 PCR-DGGE법을 이용한 미생물 군집 해석
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