Abstract ▼ AI-Helper

The alkaloid constituents of Coptidis Rhizoma and Phellodendri Cortex, Coptis japonica Makino(Ranunculaceae) and Phellodendron amurense Ruprecht(Rutaceae), are tertiary and quaternary ammonium alkaloids. These alkaloids is confirmed by its reaction with tetrathiocyanatocobaltate[Ⅱ] complex ion(TCR) ...

The alkaloid constituents of Coptidis Rhizoma and Phellodendri Cortex, Coptis japonica Makino(Ranunculaceae) and Phellodendron amurense Ruprecht(Rutaceae), are tertiary and quaternary ammonium alkaloids. These alkaloids is confirmed by its reaction with tetrathiocyanatocobaltate[Ⅱ] complex ion(TCR) to produce a blue precipitate, based on the complex formation with TCR reagent. The TCR complexes of alkaloid could be identified by colour test, ultra-violet and visible(UV-Vis.) absorption wavelength, thin layer chromatography(TLC) and high performance chromatography(HPLC). The spectrophotometric method for determination of alkaloids in Coptidis Rhizoma preparations, Gam-Cho-Sa-Sim-Tang(GCSS-Tang), Hwang-Ryen-A-Kyo-Tang(HRAK-Tang) and Phellodendri Cortex preparations, Chi-Za-Baik-Pi-Tang (CZBP-Tang), Chil-Mul-Kang-Ha-Tang(CMKH-Tang), has been established with organic solvent extraction. Alkaloids were reacted with TCR ion to form a stable ion pair. The complex compounds of alkaloids were insoluble in water but freely soluble in several organic solvents. 1,2-dichloroethane(DCE) and chloroform were found to be the best extracting solvent among others. Spectrophoto-metric determination at λ_(max). 625nm was carried out. Linearity was found between absorbance(abs. 0.409~0.613) and concentration of alkaloids in the range of 0.2~0.3 mg/mL in DCE. The method has been found to be routinely for the isolation, identification or determination of tertiary amino and quaternary ammonium alkaloids, Coptidis Rhizoma and Phellodendri Cortex preparations.

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