Abstract ▼ AI-Helper

The antioxidant effect, protective effect on oxidative damage, and anti-diabetic effect of Corni Fructus(Cornaceae) extracts by processing method were investigated in vitro and cell culture system. Following procedure of heating time, the Corni Fructus extracts were divided to the original Corni Fru...

The antioxidant effect, protective effect on oxidative damage, and anti-diabetic effect of Corni Fructus(Cornaceae) extracts by processing method were investigated in vitro and cell culture system. Following procedure of heating time, the Corni Fructus extracts were divided to the original Corni Fructus(CF-O), and Corni Fructus processed by the method 1(CF-M1) and method 2(CF-M2). The content of loganin compound as a standard component of Corni Fructus was analyzed by HPLC, showing highly in the CF-M1 extract. The antioxidant effect of Corni Fructus extracts by processing method on DPPH(1,1-diphenyl1-2- picrylhydrazyl) radical showed significantly and dose-dependently the radical scavenging activity in the CF-O, CF-M1, and CF-M2 extract. The protective effect of Corni Fructus extracts on AAPH-induced oxidative damage was examined using mouse renal mesangial cells(MMC) and the cell viability showed relatively the most effective recovery activity in the CF-M1 and CF-M2 extract by processing method as comparison to CF-O extract. The inhibitory effect of Corni Fructus extracts on advanced glycation end-products(AGE), results of glucose-mediated protein damage in diabetes, showed only in the CF-O extract as compared with control, not reduced in the CF-M1 and CF-M2 extract. The inhibitory effect of Corni Fructus extracts on α-glucosidase activity showed dose-dependently in the CF-O, CF-M1, and CF-M2 extract and the CF-O extract showed the most effective reduction. In conclusion, this data suggest that the original Corni Fructus extract in despite of change of loganin compound by processing method shows the most antioxidant and anti-diabetic effect in vitro condition.