Asthma is a chronic inflammatory disease of the airways, characterized by airway eosinophilia, goblet cell hyperplasia with mucus hypersecretion, and airway hyperresponsiveness (AHR) to both inhaled allergens and nonspecific stimulation. Eosinophils, Th2 cells, NKT cells, B cells and mast cells are ...
Asthma is a chronic inflammatory disease of the airways, characterized by airway eosinophilia, goblet cell hyperplasia with mucus hypersecretion, and airway hyperresponsiveness (AHR) to both inhaled allergens and nonspecific stimulation. Eosinophils, Th2 cells, NKT cells, B cells and mast cells are important for the pathogenesis of asthma, release of inflammatory mediators including chemokines, histamine, leukotrienes, cytokines, and IgE after exposure to allergen. The present study was performed to investigate the inhibitory effect of Radix Asteris extract (RAE) on asthma using OVA-induced murine model. C57BL/6 mice after i.p. OVA sensitization (day 0 and 7) were challenged with OVA into the trachea on day 14. RAE was administered for 8 weeks, and the blockade of the airway inflammatory response to OVA was assessed 24 hr after the last OVA treatment. Eosinophil proliferation in the control group was increased above the normal group, then RAE administration suppressed the extent of cell proliferation. AHR in response to methacoline was increased in the control group, and then decreased by RAE treatment. Histological analysis showed that the extent of fibrosis, which was developed in the lung by OVA challenge, was much reduced in RAE-treated group similar to normal tissue. Lung weight, total cell and eosinophil numbers in lung tissue and bronchoalveolar lavage fluid (BALF) were elevated in the control group in comparison to the normal group, and then determination of the same parameters in RAE-treated experimental group showed a decrease compared to the control group. Flow cytometer analysis showed that immune cell numbers (CD3e-/CCR3+, CD3e+/CCR3+, CD4+/CD8-, CD3+/CD69+, DX5+/CD3+, Gr-1+/C D11b+, IgE+/B220+) in spleen, lung, lymph node and BALF of the control group were higher than those in the normal group. However, immune cell numbers under the same criteria above were decreased in the experiment groups. The blockade of airway eosinophilia and AHR is known to be associated with reduced levels of IL-4, IL-5, IL-13 and IgE in the BALF as well as reduced serum levels of histamine. Levels of IL-4, IL-5, IL-13, IgE in the BALF and histamine in the serum, all of which were elevated in the control group, were decreased by RAE treatment in asthma-induced mice. OVA inhalation and immunization increased airway responsiveness followed by eotaxin2, CCR3 and IL-13 mRNA expression in the lung and spleen tissue as well as TNF-α, TARC and CXCR6 mRNA expression in the lung tissue. Then, the treatment of RAE during OVA inhalation significantly reduced all those mRNA expression. These data suggest that RAE alleviate allergic airway remodeling and inflammation and further can be used as a therapeutic target in asthma.
Asthma is a chronic inflammatory disease of the airways, characterized by airway eosinophilia, goblet cell hyperplasia with mucus hypersecretion, and airway hyperresponsiveness (AHR) to both inhaled allergens and nonspecific stimulation. Eosinophils, Th2 cells, NKT cells, B cells and mast cells are important for the pathogenesis of asthma, release of inflammatory mediators including chemokines, histamine, leukotrienes, cytokines, and IgE after exposure to allergen. The present study was performed to investigate the inhibitory effect of Radix Asteris extract (RAE) on asthma using OVA-induced murine model. C57BL/6 mice after i.p. OVA sensitization (day 0 and 7) were challenged with OVA into the trachea on day 14. RAE was administered for 8 weeks, and the blockade of the airway inflammatory response to OVA was assessed 24 hr after the last OVA treatment. Eosinophil proliferation in the control group was increased above the normal group, then RAE administration suppressed the extent of cell proliferation. AHR in response to methacoline was increased in the control group, and then decreased by RAE treatment. Histological analysis showed that the extent of fibrosis, which was developed in the lung by OVA challenge, was much reduced in RAE-treated group similar to normal tissue. Lung weight, total cell and eosinophil numbers in lung tissue and bronchoalveolar lavage fluid (BALF) were elevated in the control group in comparison to the normal group, and then determination of the same parameters in RAE-treated experimental group showed a decrease compared to the control group. Flow cytometer analysis showed that immune cell numbers (CD3e-/CCR3+, CD3e+/CCR3+, CD4+/CD8-, CD3+/CD69+, DX5+/CD3+, Gr-1+/C D11b+, IgE+/B220+) in spleen, lung, lymph node and BALF of the control group were higher than those in the normal group. However, immune cell numbers under the same criteria above were decreased in the experiment groups. The blockade of airway eosinophilia and AHR is known to be associated with reduced levels of IL-4, IL-5, IL-13 and IgE in the BALF as well as reduced serum levels of histamine. Levels of IL-4, IL-5, IL-13, IgE in the BALF and histamine in the serum, all of which were elevated in the control group, were decreased by RAE treatment in asthma-induced mice. OVA inhalation and immunization increased airway responsiveness followed by eotaxin2, CCR3 and IL-13 mRNA expression in the lung and spleen tissue as well as TNF-α, TARC and CXCR6 mRNA expression in the lung tissue. Then, the treatment of RAE during OVA inhalation significantly reduced all those mRNA expression. These data suggest that RAE alleviate allergic airway remodeling and inflammation and further can be used as a therapeutic target in asthma.
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#기도(신체조직)[氣道] 천식[喘息] 한의학[韓醫學]
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