Abstract ▼ AI-Helper

The experiments in this study were carried out to produce haploid plants to verify systematic breeding program and genetic analysis. To obtain the haploid plants, several treatments and factors were investigated, and the results are as follows : Microscopic investigation of the explants (Lilium Asia...

The experiments in this study were carried out to produce haploid plants to verify systematic breeding program and genetic analysis. To obtain the haploid plants, several treatments and factors were investigated, and the results are as follows : Microscopic investigation of the explants (Lilium Asiatic hybrid ‘Dreamland’) revealed that the length of flower bud in 23~24.9mm, 25~26.9mm, and 27~28.9 mm are coincided with tetrad, uninucleate, and binucleate, respectively. When the relationship between the efficiency of the anther culture from microgametogenetic stages were tested, uninucleate to early binucleate stage having the length of 23 to 28mm in flower bud revealed as the best. The frequencies of the callus induction and plant regeneration from just mentioned above stage were 17.8% and 6.7%, respectively. Though the incorporation of various plant growth regulators on culture media did not respond at significant level, the combination of picloram and zeatin together seem to be effective for the callus induction and the frequency of callus formation reached to 5%. Among the tested media such as 1/2MS, MS, N6, B5 and NLN. N6 medium showed the best as 33% callus induction and 15.3% plantlet regeneration, respectively. When calli were cultured on the MS medium containing picloram and zeatin at 25℃ for 40days, number of multiple shoots were obtained. Some regenerated plantlets were confirmed as haploid by analysis of Flow cytometer and microscopic observation.

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