PartⅠ Transdermal Delivery of FITC-OVA by Using Microneedle Transdermal drug delivery system is allowed to delivery drug within narrow limits. For instance, high molecular weight drug and crystallized drug were restricted to transfer into stratum corneum of skin because of limited space between corn...
PartⅠ Transdermal Delivery of FITC-OVA by Using Microneedle Transdermal drug delivery system is allowed to delivery drug within narrow limits. For instance, high molecular weight drug and crystallized drug were restricted to transfer into stratum corneum of skin because of limited space between corneous tissues. To solve problem, physical, chemical and bio-chemical methods were tried in the many studies. Physical methods are iontophoresis, electrophoresis, sonophoresis and microneedle and so on. Iontophoresis and electrophoresis use electricity, for example, iontophoresis use low electric current (0.5mA/cm2) and electrophoresis use high voltage (100~1,000 V). Microneedle system uses a microneedle arrays, sized from several tens to hundreds. Microneedle can make the visible holes from skin surface to inner skin. Chemical methods are chemical enhancer, conversion of epidermal tissue characterizationand manufacturing of pro-drug of drug, effects to skin directly. In the study, we used roller type of microneedle systems which can make many holes several times and use in semi-permanent to increase effect of delivering of drug through skin. We chose OVA as model drug and labeled it with FITC (Fluorescein isothiocyanate) for effective analysis. FITC-OVA was mixed in carbopol hydrogel and applied on skin which was located on franz-diffusion cell. As increasing from 2 hours to 12 hours, we took a sample of PBS in the franz-diffusion cell at intervals of two hoursand analyzed concentration by fluorescent spectrophotometer. To confirm the shape of FITC-OVA absorption, skin was harvested and made to paraffin block, then slice it 10mm. Fluorescein microscope was used to confirm the shape. Part Ⅱ Detection of Antibody after Application of OVA Hydrogel on Microneedle treated Skin In the many studies, there was demonstrated delivering of high molecular drug; protein, peptide, DNA through the skin was limited because of barrier effect of stratum corneum. The reason is that holes between keratinocytes were too small to absorb in the epidermal layer. For example, a kind of physical methods; iontophoresis, electrophoresis, sonophoresis, microneedle system and chemical methods; plasticization of lipid, making the prodrug with drug, application of chemical enhancer have been used to solve the problem of absorption limitation of permeation. In this study, we try to use a type of roller microneedle system for increasing permeation rate through skin. First, we rolled microneedle on the skin surface and found a lots of holes of microneedle shape. Second, we made the hydrogel with OVA which is high molecular weight and make a rat immune by injection and carbopol as the water absorbed polymer. Third, we totally prepared 27 BALB/C mice; control (n=3x3), injection (n=3x3), hydrogel (n=3x3) and treated on skin surface of mice in hydrogel group by using microneedle system. Finally, we gathered the blood of three mice of each of group after 10 days, 20 days and 30 days and analyzed Optical density by ELISA (Enzyme-Linked Immunosorbent Assay). In the result, we confirmed that Antibody against OVA could be made as treating skin surface by treating microneedle in the blood. The Optical density of hydrogel group was higher than injection group.
PartⅠ Transdermal Delivery of FITC-OVA by Using Microneedle Transdermal drug delivery system is allowed to delivery drug within narrow limits. For instance, high molecular weight drug and crystallized drug were restricted to transfer into stratum corneum of skin because of limited space between corneous tissues. To solve problem, physical, chemical and bio-chemical methods were tried in the many studies. Physical methods are iontophoresis, electrophoresis, sonophoresis and microneedle and so on. Iontophoresis and electrophoresis use electricity, for example, iontophoresis use low electric current (0.5mA/cm2) and electrophoresis use high voltage (100~1,000 V). Microneedle system uses a microneedle arrays, sized from several tens to hundreds. Microneedle can make the visible holes from skin surface to inner skin. Chemical methods are chemical enhancer, conversion of epidermal tissue characterizationand manufacturing of pro-drug of drug, effects to skin directly. In the study, we used roller type of microneedle systems which can make many holes several times and use in semi-permanent to increase effect of delivering of drug through skin. We chose OVA as model drug and labeled it with FITC (Fluorescein isothiocyanate) for effective analysis. FITC-OVA was mixed in carbopol hydrogel and applied on skin which was located on franz-diffusion cell. As increasing from 2 hours to 12 hours, we took a sample of PBS in the franz-diffusion cell at intervals of two hoursand analyzed concentration by fluorescent spectrophotometer. To confirm the shape of FITC-OVA absorption, skin was harvested and made to paraffin block, then slice it 10mm. Fluorescein microscope was used to confirm the shape. Part Ⅱ Detection of Antibody after Application of OVA Hydrogel on Microneedle treated Skin In the many studies, there was demonstrated delivering of high molecular drug; protein, peptide, DNA through the skin was limited because of barrier effect of stratum corneum. The reason is that holes between keratinocytes were too small to absorb in the epidermal layer. For example, a kind of physical methods; iontophoresis, electrophoresis, sonophoresis, microneedle system and chemical methods; plasticization of lipid, making the prodrug with drug, application of chemical enhancer have been used to solve the problem of absorption limitation of permeation. In this study, we try to use a type of roller microneedle system for increasing permeation rate through skin. First, we rolled microneedle on the skin surface and found a lots of holes of microneedle shape. Second, we made the hydrogel with OVA which is high molecular weight and make a rat immune by injection and carbopol as the water absorbed polymer. Third, we totally prepared 27 BALB/C mice; control (n=3x3), injection (n=3x3), hydrogel (n=3x3) and treated on skin surface of mice in hydrogel group by using microneedle system. Finally, we gathered the blood of three mice of each of group after 10 days, 20 days and 30 days and analyzed Optical density by ELISA (Enzyme-Linked Immunosorbent Assay). In the result, we confirmed that Antibody against OVA could be made as treating skin surface by treating microneedle in the blood. The Optical density of hydrogel group was higher than injection group.
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#transdermal OVA microneedle
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