消炎 抗菌작용을 가진 대표적 약재 중의 하나인 金銀花를 Lactobacillus casei PM1을 利用하여 醱酵한 醱酵 金銀花가 毒感의 原因균인 influenza virus에 대한 豫防 및 治療 효능을 細胞 次元에서 관찰 한 결과 다음과 같은 결론을 얻었다. 1. 金銀花 발효액을 농도별로 실시한 세포 독성검사에서 10, 100, 1000, 10000...
消炎 抗菌작용을 가진 대표적 약재 중의 하나인 金銀花를 Lactobacillus casei PM1을 利用하여 醱酵한 醱酵 金銀花가 毒感의 原因균인 influenza virus에 대한 豫防 및 治療 효능을 細胞 次元에서 관찰 한 결과 다음과 같은 결론을 얻었다. 1. 金銀花 발효액을 농도별로 실시한 세포 독성검사에서 10, 100, 1000, 10000 ㎍/ml 농도에서는 세포독성을 보이지 않았다. 2. Influenza virus 감염 前後 金銀花 발효액을 투여 한 결과 virus 복제를 抑制 시키는 효과적인 농도와 시간은 1000 ㎍/ml와 24시간 째이며, 前處理의 경우는 27%, 後處理의 경우는 80%의 減少率을 보였다. 3. Influenza virus와 金銀花 발효액을 직접 혼합하여 실험한 결과 virus복제 억제 효과가 있었다.
消炎 抗菌작용을 가진 대표적 약재 중의 하나인 金銀花를 Lactobacillus casei PM1을 利用하여 醱酵한 醱酵 金銀花가 毒感의 原因균인 influenza virus에 대한 豫防 및 治療 효능을 細胞 次元에서 관찰 한 결과 다음과 같은 결론을 얻었다. 1. 金銀花 발효액을 농도별로 실시한 세포 독성검사에서 10, 100, 1000, 10000 ㎍/ml 농도에서는 세포독성을 보이지 않았다. 2. Influenza virus 감염 前後 金銀花 발효액을 투여 한 결과 virus 복제를 抑制 시키는 효과적인 농도와 시간은 1000 ㎍/ml와 24시간 째이며, 前處理의 경우는 27%, 後處理의 경우는 80%의 減少率을 보였다. 3. Influenza virus와 金銀花 발효액을 직접 혼합하여 실험한 결과 virus복제 억제 효과가 있었다.
Objective : Lonicerae Flos has detoxifying property and been used as antipyretic, antibacterial and antitumor. Fermentation of herbal medicine is known to increase the absorbtion, enhance effectiveness, decrease herbal toxity and reduce side-effects. So this study was performed to the effects of fer...
Objective : Lonicerae Flos has detoxifying property and been used as antipyretic, antibacterial and antitumor. Fermentation of herbal medicine is known to increase the absorbtion, enhance effectiveness, decrease herbal toxity and reduce side-effects. So this study was performed to the effects of fermented Lonicerae Flos on influenza A/WSN (H1N1) virus replication. Material and Methods : Lonicerae Flos was fermented by Lactobacillus casei PM1. Fermented Lonicerae Flos was treated for 12 hours to MDCK (Mardin Darby Canine Kidney) cells, then cell-virulence were observed by MTT assay for 12 hours, 24 hours, and 36 hours after the treatment. Also following cases were conducted for 0, 10, 100, 1000 ㎍/ml concentration of fermented Lonicerae Flos under same time-frame; The fermented Lonicerae Flos was treated to MDCK cells before and after contaminating by A type influenza virus. The fermented Lonicerae Flos and the virus was mixed directly. The influence were observed by MTT assay and plaque assay. Results : These findings suggest that the fermented Lonicerae Flos inhibited the virulence of influenza A virus in MDCK cells and suppressed the plaque forming colonies induced by influenza A virus. Furthermore, pretreatment of fermented Lonicerae Flos was more effective than its post-treatment. The titer of influenza virus was reduced for all before and after influenza A virus inoculation.
Objective : Lonicerae Flos has detoxifying property and been used as antipyretic, antibacterial and antitumor. Fermentation of herbal medicine is known to increase the absorbtion, enhance effectiveness, decrease herbal toxity and reduce side-effects. So this study was performed to the effects of fermented Lonicerae Flos on influenza A/WSN (H1N1) virus replication. Material and Methods : Lonicerae Flos was fermented by Lactobacillus casei PM1. Fermented Lonicerae Flos was treated for 12 hours to MDCK (Mardin Darby Canine Kidney) cells, then cell-virulence were observed by MTT assay for 12 hours, 24 hours, and 36 hours after the treatment. Also following cases were conducted for 0, 10, 100, 1000 ㎍/ml concentration of fermented Lonicerae Flos under same time-frame; The fermented Lonicerae Flos was treated to MDCK cells before and after contaminating by A type influenza virus. The fermented Lonicerae Flos and the virus was mixed directly. The influence were observed by MTT assay and plaque assay. Results : These findings suggest that the fermented Lonicerae Flos inhibited the virulence of influenza A virus in MDCK cells and suppressed the plaque forming colonies induced by influenza A virus. Furthermore, pretreatment of fermented Lonicerae Flos was more effective than its post-treatment. The titer of influenza virus was reduced for all before and after influenza A virus inoculation.
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.